Method for detecting chlorpheniramine maleate and other chemical drugs in herbal beverages
A technology for chlorpheniramine acid and beverages, which is applied in the field of detecting chlorpheniramine maleate and other chemical drugs in herbal beverages, can solve the problems of rapid determination of chemical drugs, inability to supervise the quality of herbal beverages, etc., and achieves fast detection speed. , the effect of good precision and high sensitivity
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[0019] 1) Preparation of herbal beverage sample solution: dissolve the solid herbal beverage with a methanol-acetonitrile mixed solution, and dilute the liquid herbal beverage with a methanol-acetonitrile mixed solution to obtain a herbal beverage sample solution;
[0020] 2) Preparation of series contrast sample solutions: first use methanol-acetonitrile mixed solution to prepare standard solution of chlorpheniramine maleate, then get multiple parts of standard solutions, dilute to different multiples with formic acid solution, obtain series contrast sample solutions;
[0021] 3) Making of the standard curve: inject the series of control sample solutions into the high performance liquid chromatography-mass spectrometer respectively to obtain chromatograms and mass spectrograms. According to the chromatograms, the concentration of the series of control sample solutions is the abscissa, and the peak area is On the ordinate, draw the standard curve to obtain the regression equati...
Embodiment 1
[0032] A method for measuring chlorpheniramine maleate and other chemical drug concentrations in solid herbal beverages, comprising the following steps:
[0033] 1) Preparation of herbal beverage sample solution: Grind and mix the solid herbal beverage, weigh 0.2g, add it to a 25mL volumetric flask, add methanol-acetonitrile mixed solution, ultrasonically extract for 15 minutes, let stand, add methanol-acetonitrile mixed solution Constant volume, shake well, accurately measure 5mL of the above-mentioned test solution in a 10mL volumetric flask, dilute to the mark with aqueous formic acid, shake well, filter, and the filtrate is the herbal beverage sample solution; (methanol, acetonitrile in methanol-acetonitrile mixed solution The volume ratio is 1:1; the formic acid solution is 0.5% formic acid aqueous solution by mass fraction)
[0034] 2) The preparation of series control sample solution: first with methanol-acetonitrile mixed solution, chlorpheniramine maleate is mixed wit...
Embodiment 2
[0050] A method for measuring chlorpheniramine maleate and other chemical drug concentrations in liquid herbal beverages, comprising the following steps:
[0051] 1) Shake the liquid herbal drink well, let it stand, weigh 10mL supernatant, add it to a 50mL volumetric flask, add 30mL methanol-acetonitrile mixed solution, ultrasonicate for 30 minutes, let cool to room temperature, add methanol-acetonitrile mixed solution to dilute to scale, shake well, accurately measure 5mL of the above-mentioned test solution in a 10mL volumetric flask, dilute to scale with formic acid aqueous solution, shake well, and filter to obtain the herbal beverage sample solution; (the volume ratio of methanol and acetonitrile in the methanol-acetonitrile mixed solution is 1:1; formic acid solution is 0.5% formic acid aqueous solution)
[0052] 2) Preparation of series reference sample solutions: first use methanol-acetonitrile mixed solution to make chlorpheniramine maleate into a standard solution wi...
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