A paraffin section method for gradient dehydration of plant material

Abstract

The invention discloses a paraffin slicing method applicable to gradient dehydrating of a plant material. The method comprises the steps of collecting and fixing a sample, dehydrating the sample, transparentizing the sample, immersing the paraffin, embedding, trimming, fixing, slicing, expanding the slices, drying, deparaffinating, and sealing. According to the method, chlorophyll in a plant bodyis completely degraded after the sample material is transparentized through dimethylbenzene, but the cell wall and cell nucleus of the plant cell are dyed red through sarranine, so that the plant sample is red, and the color of the sample is extremely easily distinguished from the color of the embedded paraffin after the sample material is transparentized through dimethylbenzene; the sample is easily embedded as requirement, moreover, the position of the material in an embedded block is clearly viewed during trimming, so that the trimming, slicing, slice expanding and microscopic examining aresimple and convenient, and the material can be avoided after being damaged by mistake; the material expanding degree can be conveniently detected during the microscopic examining, and the slice quality and the test efficiency can be improved.

Description

technical field [0001] The invention relates to the technical field of botany, in particular to a paraffin section method for gradient dehydration of plant materials. Background technique [0002] Paraffin section is the main experimental method of histology and developmental biology research, and also an important means of observing pathological changes in pathology, which has made outstanding contributions to scientific research and clinical diagnosis. This technology uses the basic principle that paraffin wax can be well combined with animal and plant tissues. After specimen collection, fixation, dehydration, transparency, wax dipping, embedding, sectioning, spreading, patching, drying, dewaxing, and dyeing , dehydration, transparency and a series of special methods to make transparent thin slices, observed under a microscope or electron microscope, can faithfully reflect the structure and morphological changes of the body; quantitatively determine the size, quantity and ...

AI Technical Summary

Problems solved by technology

However, after adding an appropriate amount of safranin to the 70% alcohol in the dehydration process to dye the material, although the efficiency of slicing in the later stages of embedding, slicing and displaying has been improved, on the one hand, the plant samples are treated with 70% alcohol. After the safranin solution is dehydrated, the alcohol concentration will be greatly reduced, and it cannot be used again in dehydration dyeing. Therefore, the alcohol dissolved in safranin will still be thrown away eventually, resulting in waste of dyestuffs and environmental pollution.

On the other hand, the dehydration process in 70% alcohol (containing red dye) is often too long, resulting in too deep staining of plant samples, which may affect the effect of some immunolocalization experiments in the later stage

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