A visual detection method for clenbuterol hydrochloride
A clenbuterol hydrochloride and detection method technology, which is applied in the field of visual detection of clenbuterol hydrochloride, can solve the problems of non-toxic damage or loss, and achieve the effects of easy preparation, good linearity and low cost
Active Publication Date: 2020-06-23
谭贵良 +1
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Problems solved by technology
Since the general cooking method cannot destroy or deplete the toxicity of "lean meat extract" remaining in pig organs and meat, food poisoning incidents caused by excessive residual clenbuterol hydrochloride frequently occur
Method used
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[0035] The present invention will be described in further detail below in conjunction with accompanying drawing and specific embodiment, and unmarked implementation condition is the conventional condition in this industry.
[0036] 1. Synthesis of thiolated clenbuterol hydrochloride aptamer and thiolated clenbuterol hydrochloride aptamer complementary oligonucleotide single strand (purchased from Shanghai Sangon Bioengineering Co., Ltd.).
[0037] The sequence of the thiolated clenbuterol hydrochloride aptamer is: 5'SH-AGC AGC ACA GAG GTC AGA TGT CATCTG AAG TGA ATG AAG GTAAAC ATT ATT TCA TTAACACCT ATG CGT GCT ACC GTG AA-3'.
[0038] The complementary oligonucleotide single-stranded sequence of thiolated clenbuterol hydrochloride aptamer is: 5'SH-TTC ACG GTA GCACGC ATAGGT GTT AAT GAA ATAATG TTT A-3'.
[0039] 2. Preparation of nano gold.
[0040] (1) Soak relevant containers with aqua regia prepared by concentrated hydrochloric acid and concentrated nitric acid with a volume r...
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Abstract
The invention discloses a visual detection method for clenbuterol hydrochloride. The visual detection method comprises steps as follows: gold particles with particle size being 15 nm plus or minus 3 nm are prepared with a sodium citrate reduction method, mercapto clenbuterol hydrochloride aptamers and mercapto clenbuterol hydrochloride aptamer single-stranded complementary oligonucleotides are linked with the gold nanoparticles, two probes are formed, the two probes are mixed uniformly with a to-be-detected substance in certain proportion, a mixture is heated and incubated for 50 min, then a salt solution is added, and a mixed solution reacts for 4-5 min; the protective effect of DNA on the gold nanoparticles can be damaged with addition of targets, the gold nanoparticles can be gathered in the high-salt environment with increase of target concentration, the color of the red solution is changed to be light, as a result, an absorption peak is widened and red-shifted to about 540 nm, atthe moment, UV-vis scanning is performed in the position of 540 nm, and detection for clenbuterol hydrochloride in the to-be-detected substance can be realized. Therefore, the method has the advantages of being high in sensitivity, rapid and convenient, and the limit of detection can reach 1 ng / mL.
Description
technical field [0001] The invention relates to a biological detection method, in particular to a visual detection method for clenbuterol hydrochloride. Background technique [0002] Clenbuterol hydrochloride, commonly known as clenbuterol, is a white crystalline powder that can promote animal growth, improve fat distribution in animals, and increase lean meat percentage; it is easily absorbed by animals, has a long residual time in animals, and is easy to accumulate. After eating meat containing clenbuterol hydrochloride, humans will experience symptoms of poisoning such as rapid heartbeat and muscle tremors in the limbs. Since the general cooking method cannot destroy or deplete the toxicity of the residual "clenbuterol" in pig organs and meat, food poisoning incidents caused by excessive residual clenbuterol hydrochloride frequently occur. Therefore, it is of great significance to protect human health to do a good job in the analysis and detection of clenbuterol hydrochl...
Claims
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IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/786
Inventor 谭贵良李向丽刘妍刘子雄王乾蕾
Owner 谭贵良