Antithrombin III determination kit and detection method thereof

An antithrombin and kit technology, applied in the field of biomedical testing, can solve the problems of large application limitations, complicated operation, inaccurate measurement, etc., and achieve the effects of strong anti-interference ability, wide application prospect and high sensitivity

Active Publication Date: 2018-02-23
NINGBO RUI BIO TECH
View PDF9 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the chromogenic substrate method also has certain defects. This method is to analyze the AT-III activity in the sample by adding exogenous thrombin, because there is also another heparin-catalyzed thrombin inhibitor—heparin cofactor II in the sample. (HCII), so the activity of determining the remaining thrombin is the remaining activity of thrombin after reacting with AT-III and HCII, therefore, this method is susceptible to the interference of HCII in the sample, resulting in inaccurate determination of AT-III

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antithrombin III determination kit and detection method thereof
  • Antithrombin III determination kit and detection method thereof
  • Antithrombin III determination kit and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1

[0039] The antithrombin Ⅲ determination kit of this embodiment is composed of the following components:

[0040] Reagent R1

[0041] Tris buffer 100mmol / L, BSA 5g / L, gelatin 50g / L, mannitol 50g / L, ammonium sulfate 1mol / L, Triton-100 1ml / L, sodium azide 1g / L, trehalose 50g / L , Heparin 10mg / L, thrombin 10ku / L.

[0042] Reagent R2

[0043] Tris buffer 100mmol / L, BSA 5g / L, PVP 10g / L, trehalose 50g / L, Tween-20 1ml / L, sodium azide 1g / L, HD-Phe-Pip-Arg-pNA·2HCl 2g / L.

Example Embodiment

[0044] Example 2

[0045] The antithrombin Ⅲ determination kit of this embodiment is composed of the following components:

[0046] Reagent R1

[0047] Tris buffer 100mmol / L, BSA 5g / L, mannitol 50g / L, ammonium sulfate 2mol / L, Triton-100 1ml / L, sodium azide 1g / L, trehalose 50g / L, heparin 20mg / L , Thrombin 10ku / L.

[0048] Reagent R2

[0049] Tris buffer 100mmol / L, BSA 5g / L, PVP 30g / L, Tween-20 1ml / L, sodium azide 1g / L, HD-Phe-Pip-Arg-pNA·2HCl 2g / L.

Example Embodiment

[0050] Example 3

[0051] The antithrombin Ⅲ determination kit of this embodiment is composed of the following components:

[0052] Reagent R1

[0053] Tris buffer 100mmol / L, BSA 1g / L, gelatin 30g / L, mannitol 30g / L, ammonium sulfate 1mol / L, Triton-100 1ml / L, sodium azide 1g / L, trehalose 50g / L , Heparin 15mg / L, Thrombin 10ku / L.

[0054] Reagent R2

[0055] Tris buffer 100mmol / L, BSA 5g / L, casein 5g / L, Tween-20 1ml / L,

[0056] Sodium azide 1g / L, HD-Phe-Pip-Arg-pNA·2HCl 2g / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biomedical examination, and relates to an antithrombin III determination kit and a detection method thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, a stabilizer, a salt, a surfactant, a preservative, trehalose, heparin and thrombin; and the reagent R2 comprises the buffer solution, the stabilizer, the surfactant, the preservative and a chromogenic substrate. A sample and deionized water are mixed according to a volume ratio of 1:(3-5) to obtain a diluted sample; and the diluted sampleis incubated for 30-60 s, the reagent R1 is added, uniform mixing and incubation are carried out for 60-100 s, the reagent R2 is added, uniform mixing is carried out, and then the obtained mixture isdetected at a wavelength of 405 nm.

Description

technical field [0001] The invention belongs to the technical field of biomedical testing, and relates to an antithrombin III assay kit and a detection method thereof. Background technique [0002] Antithrombin Ⅲ (AT-Ⅲ) is a single-chain glycoprotein, which belongs to α2 globulin. AT-Ⅲ has the function of decomposing lipoproteins and inhibits almost all active serine proteases in the coagulation system. AT-III, as the most important inhibitor of active coagulation factors in blood, controls blood coagulation and fibrin dissolution. The level of antithrombin III in the blood varies depending on various diseases and symptoms. Reduced in disseminated intravascular coagulation (DIC), liver disease, nephrotic syndrome, etc. Decreased levels of antithrombin III in the blood may prevent the effect of heparin therapy. Therefore, it is of great significance to know the activity of antithrombin III as an index for the monitoring, pathological analysis, prognosis judgment and hepari...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 王卫华谢蒙张闻陈媛周海滨
Owner NINGBO RUI BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products