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Micro fluid control equipment used for biochemical detection

A biochemical detection and microfluidic technology, applied in the field of microfluidics, can solve the problems of affecting the detection effect and insufficient contact of the reaction fluid, and achieve the effects of good reproducibility, thorough reaction, and full contact and mixing.

Active Publication Date: 2018-02-27
TAIZHOU BOYING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the existing microfluidic chips are immobilized biomolecules on the carrier (such as substrate, membrane, etc.) as the reaction area. After the fluid flows through the reaction area, it reacts in the detection area. May affect the detection effect

Method used

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  • Micro fluid control equipment used for biochemical detection
  • Micro fluid control equipment used for biochemical detection
  • Micro fluid control equipment used for biochemical detection

Examples

Experimental program
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Effect test

Embodiment 1

[0031] This example specifically illustrates the structure of the microfluidic device of the present invention.

[0032] Such as Figure 1-3 As shown, the microfluidic device of the present invention includes a fluid channel chip and a detection area 1; the fluid channel chip is used for fluid sampling and mixing, and the first micropore 31 is provided on the fluid channel chip; The detection liquid enters the detection area 1 through the first micropore 31 for detection.

[0033] Such as Figure 1-3 As shown, the detection zone structure in this embodiment includes two detection units.

[0034] image 3 It is a schematic diagram of the disassembly of the detection area structure including two detection units, and the detection area structure includes a cover sheet 11, a first substrate 12, a second substrate 13 and a third substrate 14 in order from top to bottom; A substrate 12 is provided with one side of microfluidic channel and the one side bonding of second substrate...

Embodiment 2

[0042] The only difference between this embodiment and Embodiment 1 is that the detection area includes at least 3 detection units, and multiple detection units are connected in series or in parallel.

[0043] Such as Figure 4 as shown, Figure 4 (a) is the detection area structure in series, the mixed fluid to be detected enters the first detection unit through the first micropore, then passes through the second...n-1th detection unit in turn, and finally passes through The micropore of the nth detection unit returns to the fluid channel chip and enters the waste liquid pool to complete the detection;

[0044] Figure 4 (b) is the detection zone structure of the parallel connection of the plurality of detection units, and a micro flow channel is also provided on the lower surface of the third substrate, and after the mixed fluid to be detected passes through the first micropore, it flows on the third substrate. The flow is split on the micro-channel and enters the first n...

Embodiment 3

[0046] This embodiment illustrates the preparation method of the detection zone 1 described in embodiment 1, and the steps are as follows:

[0047] (1) Adopt conventional technology to arrange micro-channels on one side of the first substrate; set micropores on the second substrate and the third substrate;

[0048] (2) Immobilize biomolecules on a large-area membrane, and then cut the large-area membrane into membrane units with biomolecules fixed as biomolecule coatings;

[0049] (3) bonding the cover sheet, the first substrate, the second substrate and the third substrate sequentially from bottom to top, and the biomolecule coating film is fixed between the second substrate and the third substrate in the form of embedding, The assembly is then fixed to the control layer.

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Abstract

The invention relates to micro fluid control equipment used for biochemical detection. The equipment comprises a fluid flow passage chip and a detection area, wherein the fluid flow passage chip is used for fluid sample introduction and mixing, and first micropores are formed in the fluid flow passage chip; mixed liquid to be detected enters into the detection area through the first micropores fordetection; the detection area structurally comprises a cover plate, a first substrate, a second substrate and a third substrate in sequence from top to bottom; the cover plate, the first substrate, the second substrate and the third substrate are bonded in sequence, micropores for a fluid to come in and out are formed in the cover plate, the first substrate, the second substrate and the third substrate, and biomolecular films are fixed among the micropores in the second substrate and the third substrate; and the mixed fluid to be detected passes through the first micropores, third micropores,the biomolecular films, second micropores and a first substrate micro-channel fluid inlet in sequence to enter into a first substrate micro-channel for reaction. According to the micro fluid controlequipment disclosed by the invention, the detection area is independent of micro-channels, and the fluid is smooth after running through the biomolecular films, so that sufficient contact and reactionof the fluid and the biomolecular films are realized.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic device for biochemical detection. Background technique [0002] Microfluidic chip is an important science and technology of this century. Microfluidic chip is a system that manipulates the flow of microscale fluids in tiny channels or structures. It can be integrated on a sheet as small as a few square centimeters. A biology or chemistry laboratory. Microfluidic chips can greatly reduce sample consumption, that is, greatly reduce costs, reduce sample processing time, and improve detection resolution / sensitivity, which has a profound impact and significance on the development of modern technology. Microfluidic chips can be applied in a wide range of fields, and are currently involved in disease diagnosis, drug screening, environmental testing, food safety and other fields. [0003] Most of the existing microfluidic chips are immobilized biomolecules on the c...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N33/53
CPCB01L3/5027B01L3/50273B01L3/502738B01L2200/10B01L2300/0861B01L2300/0887B01L2300/12B01L2300/16B01L2400/0487G01N33/5302
Inventor 葛瑞金杨昕阎玉川张继超
Owner TAIZHOU BOYING BIOTECHNOLOGY CO LTD
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