Method for screening algal toxin degrading bacterium from viscera of hemibarbus maculatus in Lake Taihu

A technology for degrading bacteria and algal toxins, which is applied in the field of microbiology for the degradation of algal toxins, can solve the problems of large energy consumption, achieve low extraction costs, significant degradation effects, and simple and feasible methods

Inactive Publication Date: 2018-02-27
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical methods to treat algae toxins consumes a lot of energy and is prone to secondary pollution

Method used

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  • Method for screening algal toxin degrading bacterium from viscera of hemibarbus maculatus in Lake Taihu
  • Method for screening algal toxin degrading bacterium from viscera of hemibarbus maculatus in Lake Taihu

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Experimental program
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Effect test

Embodiment 1

[0033] Inoculate the bacterial solution into an Erlenmeyer flask containing 20mL of M9 liquid medium supplemented with MC-LR as a substrate, the pH is 7-7.4, the inoculation amount is 3%, 30°C, 150r min -1 Shake the culture at constant temperature, take samples every 24 hours, use enzyme-linked immunosorbent assay to measure the content of MC-LR, and investigate the degradation effect of the strain for 7 days. The degradation results showed that within 7 days the strain could convert the initial concentration of 13.98 μg L -1 MC-LR was degraded to 1.43 μg L-1 , the degradation rate is 89.72%, and the degradation effect is remarkable.

Embodiment 2

[0035] Inoculate the bacterial solution in a conical flask containing 20 mL of M9 liquid medium supplemented with MC-LR as a substrate, the inoculum amount is 3%, the pH is 5.5, 7.0, 8.5, 30°C, 150r min -1 Shake culture at constant temperature, and after 5 days, use enzyme-linked immunosorbent assay to measure MC-LR content. The degradation results showed that when the pH was 5.5, 7.0 and 8.5, the initial concentration of 13.98 μg L -1 MC-LR was degraded to 9.25 μg L -1 , 6.24 μg L -1 , 7.34 μg L -1 , the degradation rates were 33.8%, 55.36%, and 47.50%, respectively, and the degradation effect was the best when the pH was 7.0.

Embodiment 3

[0037] The bacterial solution was inoculated in a conical flask equipped with 20 mL of M9 liquid medium supplemented with MC-LR as a substrate, the pH was 7-7.4, the inoculum size was 3%, and the amount of MC-LR added was 5 μg L -1 , 10 μg L -1 , 20 μg L -1 , 30 μg L -1 , 30℃, 150rmin -1 Shake culture at constant temperature, and after 5 days, use enzyme-linked immunosorbent assay to measure the content of MC-LR. The degradation results showed that after 5 days, the remaining MC-LR content in the mixture was 0.21 μg L -1 , 1.56 μg L -1 , 5.67 μg L -1 , 13.21 μg L -1 , the degradation rates were 95.8%, 84.40%, 71.65%, and 55.97%, respectively. It can be seen that the amount of MC-LR added was 20 μg L -1 The degradation efficiency is higher when it is below.

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Abstract

A method for screening an algal toxin degrading bacterium from viscera of hemibarbus maculatus in Lake Taihu belongs to the field of MC-LR degrading microbiology. Aiming at problems of harmful algae outbreak and serious pollution of algal toxin in a water body, MC-LR extracted from dry algae powder of blue-green algae in the water body of Lake Taihu is taken as the only carbon source and nitrogensource, a bacterial strain JZ-4, namely Aeromonas salmonicida subsp.salmonicida, having relatively high MC-LR degrading capacity is screened out from the viscera of hemibarbus maculatus in Lake Taihu,and the 7d degradation rate is 89.72%. The influence of different pH conditions and different MC-LR adding amounts on the MC-LR degrading capacity of the bacterial strain is observed and studied. Theinvention provides the novel algal toxin degrading bacterial strain, and the optimum MC-LR degrading conditions are observed and studied, thereby providing theory and application references for waterbody microcystis algal toxin treatment by bioanalysis.

Description

technical field [0001] The invention belongs to the microbiological field of algae toxin degradation, and relates to a method for screening algae toxin-degrading bacteria from the viscera of surf fish in Taihu Lake. Background technique [0002] With the development of the economy and the progress of society, a large amount of N and P substances enter the water body, resulting in more and more eutrophication of the water body, and frequent outbreaks of harmful algal blooms (HAB), which pose a great threat to the water environment. On the one hand, it will lead to the decline of dissolved oxygen in the water body, which seriously restricts the growth of aquatic organisms; on the other hand, cyanobacteria cells will release algal toxins into the water body, among which microcystins (MCs for short) are the most harmful algal toxins. It has been reported that there are more than 80 variants of MCs, among which MC-LR is the most widely distributed and most toxic, which is the foc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/01
CPCC02F3/34C12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 张文艺董小娜毛林强陈泽慧郭惠娟
Owner CHANGZHOU UNIV
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