Denitrification strain taking low-quality carbon source phenol as electron donor and application thereof
A carbon source phenol and electron donor technology, applied in the direction of bacteria, chemical instruments and methods, and microbial-based methods, can solve the problems of increasing the operating cost of power-consuming aeration process, long degradation cycle, and low degradation efficiency. The effect of reducing oxygen consumption and aeration section, saving economic cost, and efficient denitrification and denitrification ability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] Screening, isolation and identification of Enterobacter sp.NJUST15.
[0020] (1) Screening and isolation of strains
[0021] Take 5g of a sample from the existing activated sludge used for denitrification and denitrification, add it into 100mL of physiological saline, stir evenly, and let it stand for two hours. Take 1 mL of the supernatant and add it to the inorganic salt medium after high temperature sterilization at 121 °C, enrich and cultivate on a shaker at 180 rpm for three days, after three consecutive enrichments, take the culture solution and dilute it with sterile water to 10 -4 -10 -10 times. To prepare the inorganic salt agar solid medium, spread 20 μL of the diluted culture solution on the inorganic salt agar solid medium, and place it in a biochemical incubator at 30° C. for three days. Select the single colonies with obvious differences on the petri dish, and use the method of plate streak separation to carry out purification and culture. After five co...
Embodiment 2
[0032] Denitrification and denitrification and phenol degradation performance of Enterobacter sp.NJUST15 strain.
[0033] Enterobacter sp.NJUST15 was inoculated to contain 100mg L -1 In the LB medium of phenol, culture on a shaker at 180 rpm at 35°C to enrich the NJUST15 strain. After the strain enters the logarithmic growth phase (about 48 hours), centrifuge the obtained bacterial solution for 10 minutes ( 6000 rpm), to obtain the deposited thalli, resuspend with the sterilized inorganic salt liquid medium, centrifuge, repeat washing three times, the bacterium is resuspended in the sterile liquid inorganic salt medium, and obtain the seed liquid (control OD 600 about 1.5).
[0034] Formulated to contain 128.3-132.4mg L -1 Nitrate nitrogen and 98.9-102.4mg L -1 The inorganic salt liquid culture medium of phenol was used as the simulated wastewater, and the above seed liquid was added to the simulated nitrate nitrogen and phenol wastewater that had been exposed to nitrogen a...
Embodiment 3
[0036] The denitrification effect of Enterobacter sp.NJUST15 in coking wastewater containing nitrate nitrogen and phenol and the degradation effect of phenol.
[0037] Insert Enterobacter sp.NJUST15 seed solution with 5% inoculum amount into the actual coking wastewater containing nitrate nitrogen and phenol after pretreatment (containing nitrate nitrogen 73.2-84.5mg L -1 , phenol 47.3-52.7mg L -1 ) in anaerobic culture at 35°C and 180 rpm. The concentration changes of nitrate nitrogen and phenol were monitored before and after wastewater treatment.
[0038] Such as image 3 As shown, Enterobacter sp. NJUST15 strain was inoculated into coking wastewater containing nitrate nitrogen and phenol after pretreatment. After about 72 hours of treatment, the removal rate of nitrate nitrogen was 100%, and phenol was completely removed after 120 hours. .
[0039] This example shows that the isolated Enterobacter sp.NJUST15 can be successfully applied to the biochemical treatment of i...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More