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Method for preparing nano-selenium from nano-aggregates of camellia plants and nano-selenium prepared by the method

A technology of nano selenium and aggregates, applied in the field of nano selenium, can solve the problems of instability and limited application scope, and achieve the effect of synergistic effect of anti-cancer activity

Active Publication Date: 2018-03-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that compared with inorganic selenium and organic selenium, nano-selenium has high biological activity and low toxicity, but its instability limits its application range, so finding a preparation method for high-stability nano-selenium has become the mainstream of research

Method used

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  • Method for preparing nano-selenium from nano-aggregates of camellia plants and nano-selenium prepared by the method
  • Method for preparing nano-selenium from nano-aggregates of camellia plants and nano-selenium prepared by the method
  • Method for preparing nano-selenium from nano-aggregates of camellia plants and nano-selenium prepared by the method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: A kind of method that utilizes camellia plant nano-aggregate to prepare nano-selenium.

[0036] (1) Accurately weigh 4.5g of green tea into a 250mL Erlenmeyer flask, add 225mL of boiling water, bathe in boiling water for 30min, shake once every 10min, suction filter while hot, cool to constant volume.

[0037] (2) Pipette 4mL of tea soup into the inner tube of a 100KDa ultrafiltration tube, centrifuge at 4000g for 20min at 4°C; collect the inner / outer tube solutions to obtain different particle size ranges (D H >10nm and D H ≤10nm) nano-aggregate solution, freeze-dried to obtain freeze-dried powder, ready to use.

[0038] (3) Prepare 10 mM sodium selenite solution and 100 mM vitamin C solution respectively.

[0039] (4) Take green tea nano-aggregate freeze-dried powder (D H >10nm) 40mg (400mg / L) in a 5mL centrifuge tube, transferred to a beaker with 10mL ultrapure water, added 72mL ultrapure water, stirred evenly, and passed through a 0.8μm filter membr...

Embodiment 2

[0042] Embodiment 2: A kind of method that utilizes camellia plant nano-aggregate to prepare nano-selenium.

[0043] Compared with embodiment 1, the difference is only in:

[0044] Weigh the freeze-dried powder of green tea nano-aggregate (D H >10nm) 2.5, 5, 10, 20, 30, 40, 50, 50mg (25, 50, 100, 200, 300, 400, 500, 600mg / L) in a 5mL centrifuge tube, transfer to Add 72mL of ultrapure water to the beaker, stir well, and pass through a 0.8μm membrane filter. The concentration of sodium selenite in the fixed system is 1mM. According to the ratio of reactants, add a certain amount of 10mM sodium selenite solution to Vc:sodium selenite 8:1 (mmol:mmol), mix well and let stand for 15min, then add A certain amount of 100mM vitamin C solution was mixed evenly, and the reaction was allowed to stand for 24 hours to obtain a nano-selenium colloidal solution. The chemical properties of the colloid were measured, and the results were as follows: figure 2 As shown, comprehensively consid...

Embodiment 3

[0045] Embodiment 3: A kind of method that utilizes camellia plant nano-aggregate to prepare nano-selenium.

[0046] Compared with embodiment 1, the difference is only in:

[0047] Weigh the freeze-dried powder of green tea nano-aggregate (D H >10nm) 40mg (400mg / L) in a 5mL centrifuge tube, transferred to a beaker with 10mL ultrapure water, added 72mL ultrapure water, stirred evenly, and passed through a 0.8μm filter membrane. The concentration of sodium selenite in the fixed system is 1mM. According to the ratio of reactants, add a certain amount of 10mM sodium selenite solution to Vc:sodium selenite 8:1 (mmol:mmol), mix well and let stand for 15min, then add A certain amount of 100mM vitamin C solution was mixed, and the reaction was allowed to stand for 4, 6, 8, 12, 24, 36, 48, and 72 hours to obtain a nano-selenium colloidal solution, and its colloidal chemical properties were measured. The results are as follows: figure 1 As shown, when the reaction time is 24h, the ave...

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Abstract

The invention belongs to the technical field of nano-materials and discloses a method for preparing nano-selenium from nano-aggregates of camellia plants. The method comprises (1) separation of nano-aggregates of camellia plants: preparing water extract of camellia plants and separating nano-aggregates of camellia plants through ultrafiltration centrifugal separation to obtain two types of colloidal particles having an average diameter of greater than 10 nm and an average diameter of less than or equal to 10 nm, and 2) preparation of nano-selenium from the nano-aggregates of camellia plants: reducing sodium selenite and vitamin C in the presence of the nano-aggregates of camellia plants to obtain nano-selenium sol and removing vitamin C to obtain a nano-selenium finished product. The method utilizes nano-aggregates of camellia plants as templates, reveals the difference of nano-selenium in the nano-aggregates of different camellia plants, enhances the functional properties of nano-selenium, broadens the application field of nano-selenium and increases a nano-selenium application value.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a method for preparing nano-selenium by using camellia plant nano-aggregates and the prepared nano-selenium. Background technique [0002] Selenium (Se) is one of the essential trace elements for the human body and is closely related to human health. Anti-oxidation and anti-cancer are the main biological activities of selenium. Selenium also has the functions of improving immunity, antagonizing heavy metals, participating in iodine metabolism in the body, regulating the level of iodine and free radicals in the body, etc. Selenium is a double-edged sword. It exerts anti-oxidant and anti-cancer activities but also produces toxicity. Biological effects of selenium and toxicity of selenium The concentration range of selenium is extremely narrow, and the toxicity is shown in a little more than the nutritional dose. The study found that the toxicity of selenium and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B19/02A61P35/00A61P29/00A61P39/06B82Y30/00
CPCB82Y30/00C01B19/02
Inventor 林晓蓉叶锡光李斌陈忠正张媛媛罗维
Owner SOUTH CHINA AGRI UNIV
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