Method for enriching effective components such as tetramethylpyrazine and fibrinolytic enzyme by fermenting coix seed

A functional ingredient, fibrinolytic enzyme technology, applied in the field of fermented barley to enrich ligustrazine and fibrinolytic enzyme functional ingredients, to achieve the effect of rich raw material sources, easy access to raw material sources, and high safety

Active Publication Date: 2018-03-16
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, no researchers have used Bacillus subtilis to ferment barley to enrich active ingredients such as ligustrazine and fibrinolytic enzymes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Strain activation:

[0030] Prepare LB slant medium: every 100ml medium contains 0.5g sodium chloride, 1g peptone, 0.3g beef extract, 1.5-2.0g agar, the rest is water, and the pH is natural.

[0031] Sterilization treatment: choose to sterilize at 121°C for 20 minutes;

[0032] Inoculation culture: under sterile conditions, inoculate Bacillus subtilis on LB medium, and cultivate and activate at 37°C for 18-22h.

[0033] (2) Seed solution culture:

[0034] Prepare LB liquid medium: every 100ml medium contains 0.5g sodium chloride, 1g peptone, 0.3g beef extract, the rest is water, and the pH is natural.

[0035] Sterilization treatment: Choose to sterilize at 121°C for 20 minutes. Under sterile conditions, inoculate the Bacillus subtilis in the LB slant medium with the LB liquid medium in the Erlenmeyer flask on a shaker at 37°C and 180rmp , constant temperature and pressure, cultivated for 20-22h, to obtain seed liquid.

[0036] (3) Fermentation:

[0037] Soak 2...

Embodiment 2

[0049]The amount of inoculation of the seed liquid in Example 1 in the wide-mouth Erlenmeyer flask is adjusted to 1ml; in the fermentation step, it is 121°C for sterilizing conditions for 30min; in the extraction step, the mass ratio of the sieved powder to the organic solvent is 1: 5; Other things remain unchanged, and the operation method of Example 1 is used for detection, and the result is: the activity of fibrinolytic enzyme is 3280.26U / g.

[0050] The measured content of Ligustrazine in the fermented product of Job's tears was 5.167 mg / g (dry basis), and the content of acetoin was 91.43 mg / g.

Embodiment 3

[0052] The amount of inoculation of the seed solution in Example 1 in the wide-mouth Erlenmeyer flask is adjusted to 1.4ml; in the extraction step, the mass ratio of the sieved powder to the organic solvent is 1:5; everything else remains unchanged, and according to Example 1 The operation method was tested, and the result was: the activity of fibrinolytic enzyme was 3866.51U / g. The measured content of Ligustrazine in the fermented product of Job's tears was 6.731 mg / g (dry basis), and the content of acetoin was 132.86 mg / g.

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PUM

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Abstract

The invention relates to the technical field of health food, particularly to a method for enriching effective components such as tetramethylpyrazine and fibrinolytic enzyme by fermenting coix seed. According to the present invention, coix seed is fermented with Bacillus subtilis, such that fibrinolytic enzyme with high fibrinolysis activity can be obtained, and tetramethylpyrazine as a calcium ionantagonist and acetoin with wide uses can be obtained; and the tetramethylpyrazine content in the fermented coix seed is more than 150 times the tetramethylpyrazine content in the soybean fermented with the same strain, the acetoin content in the fermented coix seed is more than 4 times the acetoin content in the soybean fermented with the same strain, and the fibrinolytic enzyme content in the fermented coix seed is equal to the fibrinolytic enzyme content in the soybean fermented with the same strain.

Description

technical field [0001] The invention relates to the technical field of health food, in particular to a method for fermenting coix seed to enrich ligustrazine and fibrinolytic enzyme functional components. Background technique [0002] Barley, also known as barley or barley rice, is commonly known as "Yaowang rice", "Huihui rice" and "Liugu rice". It has been a reputable nourishing food since ancient times. According to laboratory analysis, barley contains as much as 18-21% protein, 4-6% fat, and 79.2% carbohydrates. It is also rich in various vitamins (especially B vitamins), amino acids and minerals needed by the human body, coixin and Coixin and other active ingredients are very nutritious. Barley has the effects of digestion, diuresis, purulence, pain relief, swelling, moisturizing, beautifying, relieving fatigue, preventing high blood pressure and promoting digestion, especially the coixate contained in it can well inhibit the growth of certain cancer cells. Therefore,...

Claims

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Application Information

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IPC IPC(8): A23L7/104A23L33/105C12N1/20C12R1/125
CPCA23V2002/00A23L7/104A23L33/105C12N1/20A23V2250/21A23V2300/14A23V2300/50
Inventor 秦礼康文安燕
Owner GUIZHOU UNIV
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