Method for preparing ginkgo biloba leaf extract and application thereof in cigarettes

A technology for Ginkgo biloba leaves and extracts, which is applied in the field of preparation of Ginkgo biloba extracts, can solve the problems of inconspicuous product characteristic aroma, inability to meet usage requirements, unsatisfactory natural feeling, etc., and achieves improved comprehensive utilization rate and high extraction efficiency. , to avoid the effect of aroma loss

Active Publication Date: 2018-03-16
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor found in the course of the research that: the ginkgo leaf extract obtained simply by the solvent extraction process, because the extraction temperature is higher, the volatile components of some ketones and esters are prone to oxidative decomposition, resulting in loss of aroma, The characteristic aroma of the product is not obvious, and the natural feeling is not ideal. Adding it to cigarettes is likely to cause negative effects such as strong irritation and more oral residues, and cannot meet the requirements for use as an additive in tobacco products.

Method used

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  • Method for preparing ginkgo biloba leaf extract and application thereof in cigarettes
  • Method for preparing ginkgo biloba leaf extract and application thereof in cigarettes
  • Method for preparing ginkgo biloba leaf extract and application thereof in cigarettes

Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) After cleaning and draining the fresh ginkgo leaves, slice them for later use, then mix the flaked ginkgo leaves with 60% ethanol solution according to the ratio of 1:10, put them into microwave extraction equipment for extraction, and set the microwave power It is 550W, and the system temperature is set to rise from room temperature to 50-65°C within 10-30 minutes. After 0.5 hours of treatment, the filtrate is collected after filtration to obtain Ginkgo biloba extract A.

[0026] (2) Collect the ginkgo leaf residue in step (1), add 5 times the water of quality, add the biological agent of 0.5% mass ratio 1:1:0.5), after stirring evenly, incubate and ferment for 6 hours at pH 4 and temperature 30°C to obtain a hydrolysis fermentation broth. Add food-grade 95% ethanol (alcohol concentration in the system is 50%) to the hydrolysis fermentation broth, reflux extraction in a constant temperature water bath at 60°C for 3 times, filter and combine the filtrates to obtain ...

Embodiment 2

[0029] (1) After fresh ginkgo leaves are cleaned and drained, slice them for later use, then mix the flake ginkgo leaves with 30% ethanol solution according to the ratio of 1:8, put them into microwave extraction equipment for extraction, and set the microwave power It is 350W, and the system temperature is set to rise from room temperature to 50-65°C within 10-30 minutes. After treatment for 1 hour, the filtrate is collected after filtration to obtain Ginkgo biloba extract A.

[0030] (2) Collect the ginkgo leaf residue in step (1), add 4 times the water of quality, add the biological agent of 0.1% mass ratio 1:1:1), after stirring evenly, incubate and ferment for 12 hours at pH 6 and temperature 50°C to obtain a hydrolysis fermentation broth. Add food-grade 95% ethanol (the alcohol concentration in the system is 40%) to the hydrolysis fermentation broth, extract twice in a constant temperature water bath at 65°C under reflux, filter and combine the filtrates to obtain Ginkgo...

Embodiment 3

[0033] (1) After fresh ginkgo leaves are washed and drained, slice them for later use, then mix the flake ginkgo leaves with 45% ethanol solution according to the ratio of 1:6, and put them into microwave extraction equipment for extraction. It is 450W, and the system temperature is set to rise from room temperature to 50-65°C within 10-30 minutes. After 0.7 hours of treatment, the filtrate is collected after filtration to obtain Ginkgo biloba extract A.

[0034](2) Collect the ginkgo leaf residue in step (1), add 3 times the water of quality, add the biological agent of 0.3% mass ratio 1:2:0.5), after stirring evenly, incubate and ferment for 9 hours at pH 5.5 and temperature 35°C to obtain a hydrolysis fermentation broth. Add food-grade 95% ethanol (alcohol concentration in the system is 35%) to the hydrolysis fermentation broth, reflux extraction in a constant temperature water bath at 70°C for 3 times, filter and combine the filtrates to obtain Ginkgo biloba extract B.

...

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Abstract

The invention provides a method for preparing ginkgo biloba leaf extract and application thereof in cigarettes. The method includes a step (1) of cleaning draining and slicing fresh ginkgo biloba leaffor use, adopting ethanol solution for microwave extraction and extraction, collecting filtrate after filtration, and obtaining ginkgo biloba leaf extracting solution A; a step (2) of collecting ginkgo biloba leaf residue in the step (1), adding water and biological agent, performing hydrolysis fermentation, adding ethanol in hydrolysis fermented liquid for constant temperature reflux extraction,and obtaining ginkgo biloba leaf extracting solution B after filtration; a step (3) of combining the ginkgo biloba leaf extracting solution A with the ginkgo biloba leaf extracting solution B, performing vacuum concentration and then obtaining the ginkgo biloba leaf extract. The prepared ginkgo biloba leaf extract for cigarettes has specific herb fragrance features of ginkgo biloba leaves. The ginkgo biloba leaf extract serves as an additive to be applied to cigarettes. The ginkgo biloba leaf extract coordinates with cigarette fragrance, the fragrance amount of the cigarettes is enriched, thenegative problem that a conventional extracting process easily causes high irritation and more oral residues is effectively solved, and the ginkgo biloba leaf extract has an effect of removing the content of free radicals in main stream smoke in the cigarettes.

Description

technical field [0001] The invention relates to the field of cigarette essence and fragrance, in particular to a preparation method of ginkgo leaf extract and its application in cigarettes. Background technique [0002] Ginkgo biloba (also known as Gongsun tree, ginkgo) is a plant of the gymnosperm phylum Ginkgoaceae, and is one of the oldest tree species on the earth today. Ginkgo biloba has the reputation of "the giant panda in the plant kingdom", and it is a "living fossil" for studying the deep geological changes of the ancient earth. It has been listed as a second-class rare protected tree species by the state. my country's ginkgo resources account for about 75% of the world's total, ranking first in the world. Ginkgo biloba is artificially cultivated in most parts of the country, mainly distributed in Hubei, Zhejiang, Anhui, Fujian, Henan, Sichuan and other provinces and cities. Thousands of ancient ginkgo trees grow in the deep mountains of Suizhou, Hubei, and it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B3/12A24B15/26C11B9/00C12P1/00C12R1/125
CPCA24B3/12A24B15/241A24B15/26C11B9/0003C12P1/00C12N1/205C12R2001/125
Inventor 孙炜炜苗丽坤陈胜蹇顺华
Owner HUBEI CHINA TOBACCO IND
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