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Stent material used for cell storage proliferation

A scaffold material and cell technology, applied in medical science, non-woven fabrics, textiles and papermaking, etc., can solve the problem of inconvenient cutting shape, achieve convenient control of size and shape, improve degradation or dissolution speed, and use convenient effect

Inactive Publication Date: 2018-03-20
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The PEG supramolecules modified by end groups can be effectively used as spine protection hydrogels, but if the hydrogels are used directly, it is not convenient to cut the shape

Method used

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  • Stent material used for cell storage proliferation
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A scaffold material for cell storage and proliferation, characterized in that: the preparation method of the scaffold material follows the steps below:

[0030] Step 1, adding ureidopyrimidone and carbodiimide to the dihydroxy-terminated PEG molecule, and using a conventional ring-forming click reaction to obtain a PEG supramolecule;

[0031] Step 2, dissolving PEG supramolecules in hexafluoroisopropanol, then adding gelatin, stirring at room temperature overnight to obtain a mixed solution;

[0032] Step 3, adding the mixed solution into an electrospinning machine for electrospinning to obtain a fiber film;

[0033] In step 4, the fiber film is vacuum-dried overnight to obtain a scaffold material.

[0034] The molecular weight of the dihydroxy-terminated PEG molecule in the step 1 is 3000.

[0035] The molar ratio of the dihydroxy-terminated PEG molecules, ureidopyrimidinone and carbodiimide in the step 1 is 1:2:2.

[0036] The conventional ring-forming click reacti...

Embodiment 2

[0044] A scaffold material for cell storage and proliferation, characterized in that: the preparation method of the scaffold material follows the steps below:

[0045] Step 1, adding ureidopyrimidone and carbodiimide to the dihydroxy-terminated PEG molecule, and using a conventional ring-forming click reaction to obtain a PEG supramolecule;

[0046] Step 2, dissolving PEG supramolecules in hexafluoroisopropanol, then adding gelatin, stirring at room temperature overnight to obtain a mixed solution;

[0047] Step 3, adding the mixed solution into an electrospinning machine for electrospinning to obtain a fiber film;

[0048] In step 4, the fiber film is vacuum-dried overnight to obtain a scaffold material.

[0049] The molecular weight of the dihydroxyl-terminated PEG molecule in the step 1 is 15,000.

[0050] The molar ratio of the dihydroxy-terminated PEG molecules, ureidopyrimidinone and carbodiimide in the step 1 is 1:2:2.

[0051] The conventional ring-forming click rea...

Embodiment 3

[0059] A scaffold material for cell storage and proliferation, characterized in that: the preparation method of the scaffold material follows the steps below:

[0060] Step 1, adding ureidopyrimidone and carbodiimide to the dihydroxy-terminated PEG molecule, and using a conventional ring-forming click reaction to obtain a PEG supramolecule;

[0061] Step 2, dissolving PEG supramolecules in hexafluoroisopropanol, then adding gelatin, stirring at room temperature overnight to obtain a mixed solution;

[0062] Step 3, adding the mixed solution into an electrospinning machine for electrospinning to obtain a fiber film;

[0063] In step 4, the fiber film is vacuum-dried overnight to obtain a scaffold material.

[0064] The molecular weight of the dihydroxyl-terminated PEG molecule in the step 1 is 8000.

[0065] The molar ratio of the dihydroxy-terminated PEG molecules, ureidopyrimidinone and carbodiimide in the step 1 is 1:2:2.

[0066] The conventional ring-forming click react...

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Abstract

The invention belongs to the field of a biological material, and particularly relates to a stent material used for cell storage proliferation and a preparation method thereof. PEG is used for being synthesized into PEG-uramido pyrimidone-imine supermolecules by a click method; glutin is added; hexafluoroisopropanol is used for preparing a clear solution; high-voltage electrostatic spinning is usedfor preparing a fiber thin film; finally, the fiber thin film is soaked in a culture solution; cells are transplanted; the material can be used as a stent. A stable physical crosslinking gel system is used; the problem of too high material degradation or dissolution speed at present is solved.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a scaffold material for cell storage and proliferation and a preparation method thereof, more specifically, a PEG hydrogel material prepared by physical crosslinking for cell scaffold. Background technique [0002] Biomaterial hydrogels for regenerative medicine and tissue engineering are diverse and complex, including natural materials and synthetic materials, each with their own unique advantages and disadvantages. Hydrogels of natural origin, such as collagen, hyaluronic acid, gelatin, artificial basement membrane, etc., are widely used in commercial extracellular matrices to provide the physiological environment required for cell growth and differentiation. However, due to the complex diversity of its components, the interaction with cells is not clear, which greatly limits the controllable application of this type of material. In addition, the diversity of co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/18A61L27/50A61L27/52D04H1/4382D04H1/728
CPCA61L27/18A61L27/222A61L27/50A61L27/52D04H1/4382D04H1/728C08L71/02
Inventor 孟庆怡许杉杉赵亮亮黄健翔
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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