Preparation of a liquid collagen filler

A filler and liquid glue technology, applied in the field of biomaterials, can solve problems such as tissue damage, reduce bleeding and perforation, have appropriate duration and good use effect.

Active Publication Date: 2020-09-29
BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other injections such as hypertonic saline will cause different degrees of tissue damage;

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Prepare 1L of 0.01mol / l hydrochloric acid solution, and dissolve 5g of type I collagen (hydroxyproline content > 9%) with a natural triple helix structure in the hydrochloric acid solution according to the mass volume ratio of 0.5%, at 1000r / min Stir for 3h.

[0045] Filter the stirred solution through a positive pressure filter to obtain a filtrate, adjust the pH value with 1N sodium hydroxide solution to 6.5, and then stir at 1200r / min for 2h to obtain a highly uniform collagen slurry.

[0046] Freeze-dry the slurry. The freezing process is to pre-freeze at -40°C for 60 minutes, then at -20°C for 120-160 minutes, and keep the temperature of the freezer at -60--50°C; the drying process is Dry at -20°C for 5 hours, at -10°C for 5 hours, at 0°C for 5 hours, at 10°C for 5 hours, and at 20°C for 12 hours. The ambient vacuum during the pre-freezing and drying stages was 200 mTorr.

[0047] Weigh 0.25g EDC and 0.072g NHS and dissolve them in the MES buffer solution with pH...

Embodiment 2

[0052] Prepare 1L of 0.001mol / l hydrochloric acid solution, and dissolve 10g of type I collagen (hydroxyproline content > 9%) with a natural triple helix structure in the hydrochloric acid solution according to the mass volume ratio of 1%, at 1300r / min Stir for 3.5h.

[0053] Filter the stirred solution through a positive pressure filter to obtain a filtrate, adjust the pH value to 6.5 with 1N sodium hydroxide solution, and then stir at 1200r / min for 2.5h to obtain a highly uniform collagen slurry.

[0054] Freeze-dry the slurry. The freezing process is to pre-freeze at -40°C for 60 minutes, then at -20°C for 120-160 minutes, and keep the temperature of the freezer at -60--50°C; the drying process is Dry at -20°C for 5 hours, at -10°C for 5 hours, at 0°C for 5 hours, at 10°C for 5 hours, and at 20°C for 12 hours. The ambient vacuum during the pre-freezing and drying stages was 200 mTorr.

[0055] Weigh 2g of EDC and 0.4g of NHS and dissolve them in MES buffer solution with a...

Embodiment 3

[0060] Prepare 1 L of 0.1 mol / L hydrochloric acid solution, and dissolve 50 g of type I collagen (hydroxyproline content > 9%) with a natural triple helix structure in the hydrochloric acid solution according to the mass volume ratio of 5%, at 1300r / min Stir for 4h.

[0061] Filter the stirred solution through a positive pressure filter to obtain the filtrate, adjust the pH value with 1N sodium hydroxide solution to 6.5, and then stir at 1200r / min for 4h to obtain a highly uniform collagen slurry.

[0062] Freeze-dry the slurry. The freezing process is to pre-freeze at -40°C for 60 minutes, then at -20°C for 120-160 minutes, and keep the temperature of the freezer at -60--50°C; the drying process is Dry at -20°C for 5 hours, at -10°C for 5 hours, at 0°C for 5 hours, at 10°C for 5 hours, and at 20°C for 12 hours. The ambient vacuum during the pre-freezing and drying stages was 200 mTorr.

[0063] Weigh 5g of EDC and 0.745g of NHS and dissolve them in MES buffer solution with ...

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Abstract

The invention discloses a preparation method of a liquid collagen filling agent. A principle component of the filling agent is an I type collagen, wherein the collagen has an integral natural three-screw structure, and the hydroxyproline content is more than 9%. I type nontoxic crosslinked collagen powder prepared from the I type collagen is particularly applicable to preparation of the liquid collagen filling agent. The prepared liquid collagen filling agent can serve as a swelling agent of endoscopic mucosal resection (EMR) or endoscopic submucosal dissection (ESD), is reasonable in swellingheight and supporting time duration, has a certain hemostasis effect during the operation and has an excellent application effect; and moreover, the liquid collagen filling agent can serve as a softtissue filling dilation agent, is finally degraded in vivo and can be completely absorbed by tissues.

Description

technical field [0001] The invention relates to the field of biomaterials, and relates to type I collagen and a liquid collagen filler prepared therefrom. Background technique [0002] At present, with the popularity of endoscopy, the detection rate of precancerous lesions and early cancers is gradually increasing. Endoscopic submucosal dissection (ESD) or endoscopic submucosal resection (EMR) has gradually become a standard minimally invasive treatment for resection of precancerous lesions and early cancers of the digestive tract, which can completely resect the lesions en bloc , thereby greatly reducing the risk of tumor recurrence. [0003] ESD surgery also has greater risks, and some complications may occur, mainly including gastrointestinal perforation and bleeding. In view of the phenomenon of perforation in patients, in order to make it safer and more convenient, most scholars at home and abroad have confirmed that submucosal injection before surgical resection can ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04A61L31/14A61L26/00
CPCA61L26/0033A61L26/0061A61L26/009A61L31/044A61L31/14A61L31/148A61L2400/04
Inventor 张慧敏富勇
Owner BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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