The invention provides a submucosal injection solution. The submucosal injection solution comprises, by weight, 0.1-3.0 parts of sodium hyaluronate, 0.001-0.02 parts of coloring agent and 90-99 parts of water. The dyeing agent is one or more of indigo carmine, toluidine blue, phenol red, Congo red and crystal violet. The submucosal injection solution has good fluidity and a long swelling effect, and the sodium hyaluronate can serve as a mechanical barrier. The lubricating effect is good, and friction caused by foreign matters in the esophagus, stomach and intestinal tract during wound healing can be reduced. Local inflammatory response and infiltration of inflammatory cells can be reduced, and bleeding and errhysis can be reduced and inhibited. A protective film is formed on the serosa and retained for a long time, so that the serosa covering the surface of an organ can be better repaired. By affecting the viability of immune cells and cytokines, the immune regulatory function is realized, and the submucosal injection solution has angiogenesis and wound healing promoting, anti-tumor, immune regulation and other physiological functions.