Device and method for label-free monitoring of cell activity based on lens-free diffraction imaging
A lens-free diffraction and cell viability technology, which is applied in tissue cell/virus culture devices, measurement devices, biochemical cleaning devices, etc., can solve the problems of non-reusable, expensive high-frequency impedance analysis equipment, and low detection accuracy , to improve the accuracy of
Active Publication Date: 2019-12-03
南京九川科学技术有限公司
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Chinese Patent Application No. 2004800402305, titled "Real-time Electronic Cell Sensing System and Its Application in Cell-Based Assays", discloses the use of cell-substrate impedance monitoring to measure cells. The device requires a special cell culture well plate, and the electrode array is processed on the insulating substrate. The processing technology is complicated and cannot be reused. The high-frequency impedance analysis equipment is also very expensive; The impedance value evaluates cell viability, which reflects the overall impedance of the cell-medium, the impedance components are complex, there are many influencing factors, it is greatly affected by the culture environment, and the detection accuracy is not high
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[0029] see figure 1 , the cell activity label-free monitoring device based on lens-free diffraction imaging of the present invention has a CO 2 Incubator 2, CO 2 There is a diffraction information collection device 1 inside the incubator 2, CO 2 The host computer 3 is located outside the incubator 2, and the diffraction information collection device 1 performs command and data transmission with the external host computer 3 through wireless communication.
[0030] see figure 2 The structure of the diffraction information collection device 1, the diffraction information collection device 1 includes a lensless diffraction imaging mechanism 5, a collection hole positioning mechanism 6, a 96-well cell culture well plate 18, a lower computer 4 and a wifi communication module 7, etc. The bottom of the diffraction information collection device 1 is a support 10, which is fixed on the CO2 On the inner wall of the incubator 2. A 96-well cell culture well plate 18 , a lensless diffr...
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The invention discloses a device and a method for the unmarked monitoring of the activity of cells based on lensless diffraction imaging. A host computer controls a collecting hole positioning mechanism to work through a slave computer, drives a lensless diffraction imaging mechanism to move to make the lensless diffraction imaging mechanism align to different culture wells in a 96-well cell culture well plate, and controls a CMOS sensing module to photograph and acquire diffraction images corresponding to all the culture wells, every image is processed to obtain a single-cell diffraction pattern in every image, the pixel intensity contrast and the pattern strip dispersity are calculated, and are compared with preset thresholds to determine whether the cell is a living cell or not, and thecell activity index is obtained through comparing the living cell quantity of a sample group with the living cell quantity of a control group. The device and the method do not need markers, and do not need biochemical reaction operation every once in a while, so the cell activity monitoring accuracy is improved.
Description
technical field [0001] The invention relates to cell activity detection technology, in particular to a device and method for label-free monitoring of cell activity using a lensless diffraction information collection device. Background technique [0002] Cell viability is an index to evaluate the effect of different drug doses on cell viability. In drug screening, toxicology, nutrition and other research fields, it is necessary to evaluate the degree to which drugs or nutrients inhibit or promote cell activity by detecting cell activity. Therefore, cell activity detection is an important means in related research fields. A routine and indispensable link in the experiment. [0003] At present, the commonly used cell viability detection methods are biochemical methods such as MTT method, trypan blue method and CCK-8 method. These methods are labeled end-point detection methods, which need to manually add markers multiple times for biochemical reaction processing within a peri...
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IPC IPC(8): C12M3/00C12M1/34C12Q1/02
CPCC12M23/12C12M41/46C12N2503/00G01N33/5008G01N2500/10
Inventor 张荣标李国晓
Owner 南京九川科学技术有限公司