Method for determining monosaccharide constitution of astragalus polysaccharide

Abstract

The invention discloses a method for determining the monosaccharide constitution of astragalus polysaccharide. The method comprises the following steps: (1) preparing an astragalus polysaccharide hydrolysis sample; (2) preparing a mixed monosaccharide standard product; (3) carrying out pre-column derivatization: respectively mixing the astragalus polysaccharide hydrolysis sample obtained in the step (1) and the mixed monosaccharide standard product obtained in the step (2) with a 0.5mol/L PMP methanol solution and a 0.3mol/L NaOH solution, adequately shaking, reacting for 35 minutes under a 75DEG C water bath condition, cooling to the room temperature, adding 0.3mol/L hydrochloric acid for neutralizing, adding chloroform, carrying out extraction and centrifugation, abandoning an organic layer, repeatedly carrying out extraction for 2-3 times to obtain an upper-layer liquid, and filtering by virtue of a 0.22-micron micro-hole filtration film, so as to respectively obtain an astragaluspolysaccharide derivatization treatment solution and a mixed monosaccharide derivatization treatment solution for later use; and (4) carrying out high performance liquid chromatography analysis: determining the monosaccharide constitution and content of astragalus polysaccharide. The method is high in sensitivity, small in sample dosage and good in stability, and the monosaccharide constitution ofastragalus polysaccharide can be determined through simple operation and relatively low cost.

Description

technical field [0001] The invention relates to the field of determination methods, in particular to the determination of monosaccharide composition of polysaccharides. Background technique [0002] Traditional Chinese medicine polysaccharides have a wide range of biological activities such as enhancing immunity, anti-stress, and anti-virus. They have been widely used in the field of animal husbandry and veterinary medicine, and are also a hot spot in the research of new veterinary drugs. However, the current status quo is that the quality control standards for veterinary traditional Chinese medicine polysaccharides are not perfect (only the total sugar content is detected), resulting in some fake and inferior polysaccharide products appearing frequently, which endangers the development of the breeding industry. Therefore, it is an important prerequisite for the creation of new polysaccharide drugs or feed additives to carry out research on the quality control of veterinary ...

AI Technical Summary

Problems solved by technology

Since polysaccharides and monosaccharides do not have UV absorption, they cannot be directly measured

When using high performance liquid chromatography to analyze the composition and content of polysaccharides, instruments such as gel chromatographic columns and differential refraction dete

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