Preparation method of allogeneic biological patch

A biological patch, allogeneic technology, applied in tissue regeneration, medical science, surgery, etc., can solve problems such as no market precedent, reduce the incidence of capsular contracture, ensure accuracy, and optimize biocompatibility. sexual effect

Active Publication Date: 2018-04-10
BEIJING QINGYUAN WEIYE BIO TISSUE ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The biological patch products that have been on the market in China are only the biological patch of Guanhao Biological Co., Ltd. (National Machinery Injection No. 20153460482) and the breast soft tissue enhancement patch of pfm medical titanium gmbh (National Machinery Injection No. 20163461539). The former is an animal source products, the latter is made of polymer materials, and biological patch products of allogeneic origin have not yet been marketed.

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  • Preparation method of allogeneic biological patch
  • Preparation method of allogeneic biological patch
  • Preparation method of allogeneic biological patch

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preparation example Construction

[0041] The first group of embodiments, the preparation method of biological patch of the present invention

[0042] The embodiment of this group provides a preparation method of allogeneic biological patch. All embodiments of this group all possess following common features: described preparation method comprises the steps:

[0043] (1) Obtain human skin tissue and obtain the dermis;

[0044] (2) placing the dermis in a fixative solution for fixation;

[0045] (3) placing the fixed dermis in a decellularized solution for decellularization to obtain decellularized dermal tissue;

[0046] The fixative is phosphate buffered saline (PBS) containing 1%-2% formaldehyde and 1.5%-3.0% o-phthalaldehyde;

[0047] The phosphate buffer, preferably sodium phosphate buffer, can also be replaced by potassium phosphate buffer; of course, those skilled in the art can also choose to use other similar phosphate buffer according to the content described herein and according to actual needs , su...

experiment example

[0062] Experimental example, the specific operation of the preparation method of the present invention and the performance detection of biological patch products

[0063] Step A: select healthy adult skin tissue, remove subcutaneous fat; soak in 1mol / L-2mol / L NaCl solution at 37°C for 24 hours to remove the epidermis to obtain the dermis;

[0064] Step B: Through mechanical processing, according to different models and specifications, make corresponding shapes, such as rectangle, sector, circle, or punch holes;

[0065] Step C: In the process of removing the dermal cell substance, the collagen constituting the frame structure in the material and the attached special factors etc. are protected, fixed and restored by biochemical and tissue engineering methods.

[0066] Step D: Utilize the patented decellularization technology, add specific enzymes and chemical reagents to the alkaline solution of 1.5mol / L-2.0mol / L NaOH or KOH in the basic preparation solution to prepare decellul...

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Abstract

The invention relates to a preparation method of an allogeneic biological patch, and belongs to the technical field of biological material preparation. The preparation method comprises the steps of 1,obtaining people's skin tissues and a corium layer; 2, placing the corium layer in a fixing solution to perform fixing treatment; 3, placing the fixed corium layer in a decellularization solution toperform decellularization treatment to obtain decellularization corium tissues. The fixing solution is a phosphate buffer solution containing 1%-2% formaldehyde and 1.5%-3.0% o-phthalaldehyde; the decellularization solution is a 1.5-2.0 mol/L NaO or KOH alkaline solution containing 10-500 U/L trypsin, 1-25 mmol/L calcium chloride and 1-25 mmol/L magnesium chloride. The allogeneic biological patchhas effective fixing and supporting effects on breast implants, and different sizes are designed to satisfy the requirement of different patients and operating methods; in addition, because extremelylow immunogenicity and extremely excellent biocompatibility of the allogeneic biological patch, it is expected that occurrence rate of diolame contracture after the breast implants are implanted can be significantly reduced.

Description

technical field [0001] The invention belongs to the technical field of biological material preparation, and in particular relates to a preparation method of an allogeneic biological patch technical background [0002] At present, the products that can be applied to breast reconstruction and plastic surgery in the domestic plastic surgery market are very mature. At the same time, there is also a huge market for biological patch products that are specially used to repair soft tissue defects, wrap breast prostheses, and suspend and stretch. need. There should be at least 300,000 cases of breast reconstruction and 100,000 cases of conventional breast augmentation surgery in China every year. Soft tissue repair patch products have taken on the needs of professional application of biological patches in clinical practice, and there is a certain degree of There is an urgent need for matching biological patch products for over-range or irregular use. [0003] At present, the most w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/00A61L31/14
CPCA61L31/005A61L31/14A61L2430/40
Inventor 李宗燕刘伟张泓李晓薇李燕青
Owner BEIJING QINGYUAN WEIYE BIO TISSUE ENG
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