Preparation method of allogeneic biological patch
A biological patch, allogeneic technology, applied in tissue regeneration, medical science, surgery, etc., can solve problems such as no market precedent, reduce the incidence of capsular contracture, ensure accuracy, and optimize biocompatibility. sexual effect
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preparation example Construction
[0041] The first group of embodiments, the preparation method of biological patch of the present invention
[0042] The embodiment of this group provides a preparation method of allogeneic biological patch. All embodiments of this group all possess following common features: described preparation method comprises the steps:
[0043] (1) Obtain human skin tissue and obtain the dermis;
[0044] (2) placing the dermis in a fixative solution for fixation;
[0045] (3) placing the fixed dermis in a decellularized solution for decellularization to obtain decellularized dermal tissue;
[0046] The fixative is phosphate buffered saline (PBS) containing 1%-2% formaldehyde and 1.5%-3.0% o-phthalaldehyde;
[0047] The phosphate buffer, preferably sodium phosphate buffer, can also be replaced by potassium phosphate buffer; of course, those skilled in the art can also choose to use other similar phosphate buffer according to the content described herein and according to actual needs , su...
experiment example
[0062] Experimental example, the specific operation of the preparation method of the present invention and the performance detection of biological patch products
[0063] Step A: select healthy adult skin tissue, remove subcutaneous fat; soak in 1mol / L-2mol / L NaCl solution at 37°C for 24 hours to remove the epidermis to obtain the dermis;
[0064] Step B: Through mechanical processing, according to different models and specifications, make corresponding shapes, such as rectangle, sector, circle, or punch holes;
[0065] Step C: In the process of removing the dermal cell substance, the collagen constituting the frame structure in the material and the attached special factors etc. are protected, fixed and restored by biochemical and tissue engineering methods.
[0066] Step D: Utilize the patented decellularization technology, add specific enzymes and chemical reagents to the alkaline solution of 1.5mol / L-2.0mol / L NaOH or KOH in the basic preparation solution to prepare decellul...
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