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A kind of preparation method and application of graphene oxide-lipid nanocomposite material

A composite material and lipid nanotechnology, which is applied in the field of material synthesis and biomedicine, can solve the problems of poor solubility and easy agglomeration, and achieve the effects of low cost, improved dispersibility, good dispersibility and stability

Active Publication Date: 2020-06-09
江苏江科石墨烯研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, unmodified graphene oxide is sensitive to solution environments such as solute, pH, and ionic strength, and has extremely poor solubility in physiological environments (such as cell culture fluid, phosphate buffer), and is prone to agglomeration. To some extent, its application is limited

Method used

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  • A kind of preparation method and application of graphene oxide-lipid nanocomposite material
  • A kind of preparation method and application of graphene oxide-lipid nanocomposite material
  • A kind of preparation method and application of graphene oxide-lipid nanocomposite material

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Effect test

Embodiment 1

[0032] (1) Use a cell breaker to crush the graphene oxide water dispersion into nanometer-sized graphene oxide water dispersions with a power of 400w and an ultrasonic time of 0.5 h in an ice-water bath; (2) Weigh egg yolk phospholipids, and Dissolve in an appropriate amount of chloroform, then measure the solution and place it in an eggplant-shaped bottle, and use a rotary evaporator to rotate it to form a film (first rotate for half a minute and then vacuum). Then add an appropriate amount of PBS solution into the container and rotate to make the film fall off; (3) Pour the contents of the eggplant-shaped bottle into a 10mL centrifuge tube, and ultrasonically disperse it to clarify it; (4) Prepare a certain amount of concentration of 1 mg / mL of graphene oxide aqueous solution. Take several 5 mL centrifuge tubes, add graphene oxide solution, liposome solution and appropriate amount of pure water respectively, the mass ratio of graphene oxide and liposome is 15:1, place in a ...

Embodiment 2

[0035](1) Use a cell breaker to crush the graphene oxide aqueous dispersion into nano-sized graphene oxide aqueous dispersions with a power of 300w and ultrasonic time of 1 h in an ice-water bath; (2) Weigh the egg yolk phospholipids, and Dissolve in an appropriate amount of chloroform, then measure the solution and place it in an eggplant-shaped bottle, use a rotary evaporator to rotate it to form a film (rotate for half a minute before vacuuming), then add an appropriate amount of PBS solution to the container and rotate to make the film (3) Pour the contents of the eggplant-shaped bottle into a 10 mL centrifuge tube, and ultrasonically disperse to clarify it; (4) Prepare a certain amount of graphene oxide aqueous solution with a concentration of 1 mg / mL, take 5 mL and centrifuge Several tubes were added with graphene oxide solution, liposome solution and appropriate amount of pure water, the mass ratio of graphene oxide and liposome was 10:1, placed in a constant temperature...

Embodiment 3

[0038] (1) Use a cell breaker to crush the graphene oxide aqueous dispersion into nanometer-sized graphene oxide aqueous dispersions with a power of 250w and an ultrasonic time of 1.5 h in an ice-water bath; (2) Weigh egg yolk phospholipids, and Dissolve in an appropriate amount of chloroform, then measure the solution and place it in an eggplant-shaped bottle, use a rotary evaporator to rotate it to form a film (rotate for half a minute before vacuuming), then add an appropriate amount of PBS solution to the container and rotate to make the film (3) Pour the contents of the eggplant-shaped bottle into a 10mL centrifuge tube, and ultrasonically disperse to clarify it; (4) Prepare a certain amount of graphene oxide aqueous solution with a concentration of 1 mg / mL, take 5 mL and centrifuge Add graphene oxide solution, phospholipid solution and appropriate amount of pure water to several tubes, the mass ratio of graphene oxide and liposome is 20:1, place it on a constant temperatu...

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Abstract

The invention discloses a preparation method of a graphene oxide-lipid nanocomposite material, belonging to the technical fields of material synthesis and biological medicines. The preparation methodcomprises the following steps: by adopting graphene oxide of the nanometer size as a matrix, synthesizing a liposome by adopting a rotary evaporation method, and carrying out lipid modification on thegraphene oxide through the electrostatic adsorption effect, and thus the composite material is obtained. The synthesized lipid modified nano-carrier has relatively good stability and dispersibility under physiological conditions, graphene oxide of the nanometer size is adopted as the core which is wrapped by a layer of liposome, and the two materials have good biocompatibility; the antitumor medicine, namely, adriamycin is loaded to graphene oxide, the medicine loading rate is high, the stability is good, the medicine is enabled to have a targeting slow-release effect, the efficacies are effectively improved, and the toxic and side effects of the medicine are reduced.

Description

technical field [0001] The invention belongs to the technical field of material synthesis and biomedicine, and in particular relates to a preparation method of a graphene oxide-lipid nanocomposite material and its application in antitumor drug carriers. Background technique [0002] Tumor is one of the refractory diseases that seriously endanger human health, and the treatment with chemical drugs has poor treatment results due to serious side effects. The use of drug carriers for targeted therapy of cancer improves the curative effect and reduces side effects, which opens up new ideas for the treatment of tumors. There are various types of anticancer drug carriers discovered so far, including organic materials (such as proteins, lipids), inorganic materials, and polymer materials. Because organic materials and polymer materials have defects such as easy degradation, easy leakage of drugs and high cost as drug carriers, in order to overcome these defects, the present inventi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K9/127A61K31/704A61P35/00
CPCA61K9/127A61K31/704
Inventor 谢萌张雅楠徐远国杨娜杨眉张峰
Owner 江苏江科石墨烯研究院有限公司