Kaolinite-loaded biosynthetic nano ferro-sulfide material as well as preparation method and application thereof
A biosynthesis and kaolinite technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of sulfate-reducing bacteria, weak adsorption, and nano-sulfur oxidation, etc. Good dispersion effect, easy to obtain, easy to recycle
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Embodiment 1
[0053] A preparation method of kaolinite-loaded biosynthetic nano-iron sulfur material, comprising the steps of:
[0054] (1) 0.3500g sodium lactate, 0.6500g KH 2 PO 4 , 1.0000g Na 2 SO 4 , 1.0000g NH 4 Cl, 2.0000gMgSO 4 ·7H 2 0 and 1.0000g of yeast extract were dissolved in 725ml of water, mixed uniformly, then adding 200ml of HEPES buffer solution with a concentration of 0.1M to obtain a mixed solution, and adjusting the pH of the mixed solution with 0.1M NaOH solution to be 6.5;
[0055] (2) Add 74.0ml of the mixed solution in step (1) into the anaerobic bottle, then add 240mg of kaolinite to obtain a suspension, blow off with nitrogen for 30min to remove the dissolved oxygen in the suspension, and then seal the anaerobic bottle. Oxygen bottle, the anaerobic bottle is sterilized (sterilized at 121° C. for 30 min), to obtain the sterilized mixed solution in the bottle;
[0056] (3) 1.0000g CaCl 2 Dissolve in 25.0ml sterilized water, mix well to get CaCl 2 solution; ...
Embodiment 2
[0062] Get 3 parts of Cr (VI) solutions that initial concentration is 0.80mM respectively in 3 reaction vessels, the background ion concentration of described Cr (VI) solutions is the NaCl of 0.10M, the pH value of described Cr (VI) solutions 6.5 (using 50.0mM HEPES as buffer), blow off with nitrogen respectively for 30min, keep the anaerobic environment; then add the pure nano iron sulfur material (FeS) that 70mg comparative example 1 makes respectively in these 3 reaction vessels, The kaolinite-loaded biosynthetic nano-iron sulfur material (kaolinite / FeS) that 600mg embodiment 1 makes, 600mg kaolinite, the FeS content in the kaolinite / FeS of 600mg embodiment 1 is 30mg, and 70mg FeS is the content of 30mg FeS 2.3 times, add the rotor, plug the butyl rubber stopper and aluminum cover to ensure its anaerobic environment, then put these 3 reaction vessels on a magnetic stirrer with a rotating speed of 200rpm to carry out the Cr(VI) removal reaction, the reaction The temperature ...
Embodiment 3
[0065] Example 3 is basically the same as Example 1, the only difference being that the amount of kaolinite described in step (2) is 24 mg, that is, the concentration of kaolinite in the culture solution described in step (3) is 0.3 mg / mL , to obtain kaolinite-loaded biosynthetic nano-iron sulfur material (kaolinite / FeS).
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