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Prebiotics composition and application thereof

A prebiotic and composition technology, applied in the field of oligosaccharide nutritional compositions, can solve the problems of reducing the fermentation speed and fermentation capacity of short-chain oligosaccharides, and achieve the enhancement of immunity and comprehensive functions of the body, good health and growth, The effect of increasing the content

Inactive Publication Date: 2018-05-04
HEBEI SANYUAN FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, mixing short-chain oligosaccharides and long-chain oligosaccharides reduces the fermentation speed and fermentability of short-chain oligosaccharides

Method used

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  • Prebiotics composition and application thereof
  • Prebiotics composition and application thereof
  • Prebiotics composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1. Experimental purpose and materials.

[0031] The purpose of the experiment: To compare the prebiotic effects of the prebiotic combination of galacto-oligosaccharide, fructo-oligosaccharide and isomerized lactose fed to mice at different dosages. The infant formula milk powder without prebiotics was used as the control, and compared with breast milk Compare.

[0032] group

[0033] Among them, the infant formula milk powder without prebiotics was produced in Hebei Sanyuan Food Co., Ltd. on a pilot basis.

Embodiment 2

[0034] Embodiment 2, intestinal microorganism detection.

[0035] Before the end of each experimental period, fresh fecal samples were taken through the anus in the morning to detect the intestinal flora. The detection principle of this method is as follows:

[0036] 16S / 18S / ITS rDNA, the sequence includes conserved regions and hypervariable regions, among which the conserved regions have little difference among microbial species, and the hypervariable regions have genus or species specificity, and have certain differences with different genetic relationships. Therefore, 16S / 18S / ITSrDNA can be used to reveal the characteristic nucleic acid sequences of biological species, and is considered to be an indicator suitable for microbial phylogeny and taxonomic identification. Nowadays, 16S / 18S / ITS rDNA amplicon sequencing technology has become an important means to study the composition and structure of microbial communities in environmental samples.

[0037] 16S rDNA amplicon seq...

Embodiment 3

[0041] Example 3, detection of short-chain fatty acids.

[0042] Preparation of fecal samples: For the determination of SCFAs, 1 g of samples were thawed in ice water, diluted 10X in MilliQ, and homogenized for 10 minutes using a stomacher (IUL Instruments, Barcelona, ​​Spain). 350 μL homogenized feces were mixed with 200 μl 5% (v / v) formic acid, 100 μL 1.25 g / L 2-ethylbutyric acid (Sigma-Aldrich, Zwijindrecht, Netherlands) and 350 μl MilliQ. Samples were centrifuged at 14,000 rpm for 5 minutes to remove large particles and supernatants were stored at -20°C. For FISH analysis and lactate measurement, samples were thawed in ice water, diluted 10X (w / v) in phosphate buffered saline, pH 7.4 (PBS) and homogenized for 10 minutes using a stomacher. Homogenized feces were stored at -20°C.

[0043] Short-chain fatty acid analysis: Quantitative determination of short-chain fatty acids (SCFA), formic acid, acetic acid, propionic acid, n-butyric acid, by a Marian 3800 gas chromatograph...

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PUM

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Abstract

The invention discloses a prebiotics composition and application thereof. The composition contains galacto-oligosaccharides (GOS), fructo-oligosaccharide (FOS) and isomeric lactose (LOS) in the weightpart ratio of (5 to 10) to (0.5 to 1.5) to (0.5 to 1.5), wherein the polymerization degree of the FOS is between 2 and 9. The prebiotics composition disclosed by the invention can be applied to nutritional foods and medicaments in order to enhance microorganisms being beneficial for the health in the intestinal flora of human body; meanwhile, the growth of harmful bacteria is restrained, so thatthe immunity and overall performance of the organism are enhanced.

Description

technical field [0001] The invention relates to the technical fields related to nutrition and health, in particular to an oligosaccharide nutritional composition in food for infants and young children and related technologies. Background technique [0002] Breast milk is the most ideal food for newborn babies. It contains nutrients such as protein, fat, carbohydrates, vitamins and minerals in an appropriate proportion. It is easy to be digested and absorbed by babies and can meet their growth and development needs. In addition, breast milk is rich in Contains immune factors such as lactoferrin and bifidus factor, which can prevent intestinal infectious diseases in infants. Among them, human milk oligosaccharides (HMOs) are the third largest nutrient in breast milk after lactose and fat. It is not destroyed by gastric acid or digested by digestive enzymes. It can directly reach the large intestine and stimulate intestinal It promotes the growth of beneficial bacteria (Bifido...

Claims

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Application Information

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IPC IPC(8): A23L33/125A23C9/156A61K31/702A61K31/733A61K31/715A61P1/00A61P37/04
CPCA23C9/156A23V2002/00A61K31/702A61K31/715A61K31/733A23L33/125A23V2200/3202A23V2200/324A23V2250/284A23V2250/612A61K2300/00
Inventor 李朝旭张天博杨凯贾云虹薛江超
Owner HEBEI SANYUAN FOOD