Composition containing miracle fruits and probiotics, and pastilles and preparation method of composition containing miracle fruits and probiotics

A technology of miracle fruit and probiotics, applied in the field of symbiosis, can solve problems such as destructive activity, achieve the effect of improving superoxide removal and protecting cells from oxidative damage

Inactive Publication Date: 2018-05-08
SENYUH FARM SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the application of the above-mentioned miracle fruit, although it has the miraculous effect of converting food acidity into sweetness, its mysterious protein will be destroyed in an environment with a pH value below 2.5 or above 12.0 or in an environment with a high concentration of organic solvents. active, so few people have been able to make it into a convenient lozenge for users to take with them

Method used

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  • Composition containing miracle fruits and probiotics, and pastilles and preparation method of composition containing miracle fruits and probiotics
  • Composition containing miracle fruits and probiotics, and pastilles and preparation method of composition containing miracle fruits and probiotics
  • Composition containing miracle fruits and probiotics, and pastilles and preparation method of composition containing miracle fruits and probiotics

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0039] Preparation example 1 culture strain

[0040]According to domestic and foreign literature data, lactic acid bacteria have many health benefits to the human body. The proven effects include improving the absorption of lactose, reducing the occurrence of diarrhea, antibacterial, lowering cholesterol, anti-cancer and enhancing immunity. Referring to the list of raw materials available for food use by the Department of Health, a total of 10 strains of lactic acid bacteria commonly used in screening were obtained from the Bioresource Collection and Research Center (BCRC) of the Taiwan Food Industry Development Institute, as shown in Table 1 below. Show.

[0041] Table 1. Ten Lactic Acid Bacteria Strains

[0042]

[0043] Take 10 per milliliter of the above 10 strains of lactic acid bacteria 4 to 10 9 Colony-forming units (colony-forming units, CFU / mL) 10 ml (ie 10 5 CFU to 10 10 CFU) were cultivated in 100mL of MRS medium, and the culture conditions were 37°C and 48 ...

preparation example 2

[0044] Preparation example 2 Miracle fruit matrix deployment

[0045] First, the pulp of the miracle fruit after the seeds are removed is freeze-dried and ground into miracle fruit powder. Preparation per unit volume is the miracle fruit aqueous solution of 0.5%, 1.0%, 1.5% and 2.0% weight (w / v), and detects that the miracle fruit aqueous solution acid-base value (pH) is respectively 3.18 (0.5%), 3.21 (1.0 %), 3.26 (1.5%), 3.33 (2.0%), its pH value is all below 3.5. Therefore, acid-base neutralization is carried out with a small amount of sodium hydroxide, so that the final pH value is 5 to 8; the preferred pH value is 6 to 7; the more preferred pH value is 6.5. Add 6.5% (w / v) milk powder (fat content is that every 100 grams of milk powder contains 28.8 grams of fat, purchased from Fenglifu TM High-quality extra-concentrated milk powder) is sterilized at 121°C for 15 minutes to obtain the miracle fruit matrix, which is uniform and free of suspension. If the pH value is not ...

Embodiment 1

[0046] Embodiment 1 Miracle fruit and lactic acid bacteria joint fermentation culture

[0047] From preparation example 1, select Lactobacillus casei 1 strain (LC1), Lactobacillus paracasei 3 strains (PC2, PC3 and PC4), Lactobacillus acidophilus 1 strain (LA1) and Streptococcus thermophilus 1 strain (SS1), take 10mL concentration is 10 8 CFU / mL to 10 9 CFU / mL (ie 10 9 CFU to 10 10 CFU) were respectively placed in 100 mL of the 0.5% (w / v) miracle fruit matrix of Preparation Example 2 and co-cultivated at 37° C. for 48 hours.

[0048] The result is as figure 2 As shown, the total number of lactic acid bacteria in each group can reach 10 8 CFU / mL or more, of which the three with higher bacterial counts are Lactobacillus paracasei PC2 with 3.1×10 9 CFU / mL, Lactobacillus paracasei PC3 is 1.9×10 9 CFU / mL and Lactobacillus casei LC1 is 2.0×10 9 CFU / mL; the two with the lower bacterial counts were Lactobacillus acidophilus LA1, which was 2.3×10 8 CFU / mL and Streptococcus the...

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Abstract

The invention provides a preparation method of a composition containing miracle fruits and probiotics. The preparation method comprises the following steps: (1) preparing miracle fruits, performing homogenization and mixing on the prepared miracle fruits and water to form a miracle fruit aqueous solution; (2) regulating the pH value of the miracle fruit aqueous solution to be 5-8; (3) adding milkpowder to the miracle fruit aqueous solution of which the pH value is regulated to form a miracle fruit substrate; and (4) performing fermentation and culturing on the miracle fruit substrate and theprobiotics for some time to obtain the composition containing the miracle fruits and the probiotics. The invention further provides the composition containing the miracle fruits and the probiotics, prepared by the preparation method. The invention further provides pastilles containing composition powder obtained after drying the composition, fruit powder and excipients. The composition containingthe miracle fruits and the probiotics notably improves superoxide dismutase-like activity and achieves the efficacy of resisting oxidation.

Description

technical field [0001] The invention relates to a symbiotic substance, in particular to a symbiotic substance containing miracle fruit and probiotics. The present invention also relates to a preparation method, in particular to a preparation method of a symbiotic substance containing miracle fruit and probiotics. The present invention also relates to a lozenge, in particular to a lozenge containing a symbiotic substance of miracle fruit and probiotics. Background technique [0002] According to statistics from the Ministry of Health and Welfare of Taiwan, China, colorectal cancer has ranked third among the top ten cancers among Chinese people for the sixth consecutive year. This is inseparable from modern people's living and eating habits. Red meat, lack of exercise, and obesity are all factors that increase the incidence of colorectal cancer. Therefore, in addition to regular inspections and increased activity levels, creating a healthy "bowel" and long-term eating habits ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/13A23C9/133
CPCA23C9/13A23C9/1315A23C9/133
Inventor 林美玲
Owner SENYUH FARM SCI
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