Construction of nanoreactor encapsulated with arginine deiminase
A technology of arginine deiminase and nano-reactor, which is applied in the field of construction of ADI-loaded nano-reactor, can solve the problems of affecting urease activity, activity reduction, short half-life, etc.
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Embodiment 1
[0040] According to the molar ratio of the structural unit of CMCNa (DD=95.2%, DS=1.02) and azide benzaldehyde (Az) is 1:1, the Az dissolved in ethanol is added dropwise in the CMCNa aqueous solution, stirred at room temperature for 8 Hour, obtain Az-CMCNa solution. The crude product was precipitated by adding 95% ethanol to the solution, washed three times with 95% ethanol (v / v), and finally dried under vacuum at 45° C. to obtain Az-CMCNa.
[0041] Use a probe-type sonicator to ultrasonically treat 0.4% (w / w) Az-CMCNa aqueous solution for 3 minutes to obtain a micellar dispersion, then add ADI to the micellar dispersion to a concentration of 30ug / ml, and then drop while stirring Dilute hydrochloric acid solution was added until the molar ratio of added hydrochloric acid to ionized carboxyl groups in Az-CMCNa was 1:1, at which point the micelles transformed into ADI-containing nanocapsules. The capsule solution was exposed to 250nm UV light for 20 seconds to induce in-situ cr...
Embodiment 2
[0043] According to the molar ratio of the structural unit of CMCNa (DD=90.0%, DS=0.62) and azidobenzaldehyde (Az) is 1:2, the Az dissolved in ethanol is added dropwise in the CMCNa aqueous solution, stirred at room temperature for 8 Hour, obtain Az-CMCNa solution. The crude product was precipitated by adding 95% ethanol to the solution, washed three times with 95% ethanol (v / v), and finally dried under vacuum at 45° C. to obtain Az-CMCNa. Use a probe-type sonicator to sonicate 0.2% (w / w) Az-CMCNa aqueous solution for 3 minutes to obtain a micellar dispersion, then add ADI to the solution to a concentration of 45ug / ml, and then add dilute hydrochloric acid dropwise while stirring solution until the molar ratio of added hydrochloric acid to ionized carboxyl groups in Az-CMCNa was 1:1, at which point the micelles transformed into ADI-containing nanocapsules. The capsule solution was exposed to 254 nm UV light for 30 seconds to induce in situ cross-linking of the AZ groups in th...
Embodiment 3
[0045] According to the molar ratio of the structural unit of CMCNa (DD=93.0%, DS=0.87) and azide benzaldehyde (Az) is 1:0.5, Az dissolved in 95% ethanol is added dropwise in CMCNa aqueous solution, at room temperature After stirring for 8 hours, Az-CMCNa solution was obtained. The crude product was precipitated by adding 95% ethanol to the solution, washed three times with 95% ethanol (v / v), and finally dried under vacuum at 45° C. to obtain Az-CMCNa. Use a probe-type sonicator to sonicate 0.1% (w / w) Az-CMCNa aqueous solution for 3 minutes to obtain a micellar dispersion, then add ADI to the solution to a concentration of 65ug / ml, and then add dilute hydrochloric acid dropwise while stirring solution until the molar ratio of added hydrochloric acid to ionized carboxyl groups in Az-CMCNa was 1:1, at which point the micelles transformed into ADI-containing nanocapsules. The capsule solution was exposed to 260 nm UV light for 20 seconds to induce in situ cross-linking of the AZ...
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