Formula of 3D-printing artificial bone composite fiber protein scaffold and preparation method of same

Abstract

The invention discloses a formula of a 3D-printing artificial bone composite fiber protein scaffold and a preparation method of same, the scaffold includes five components: medical-grade collagen powder, a 0.05 mol / L acetic acid solution, medical-grade hydroxylapatite powder, a 1 mass% genipin solution, and platelet-rich fibrin. The preparation method includes the steps of: 1) dissolving the medical-grade collagen powder in the 0.05 mol / L acetic acid solution to obtain collagen colloid; 2) uniformly stirring the medical-grade hydroxylapatite powder and collagen colloid to prepare a hydroxylapatite / collagen printing raw material, and according to bone defect CT data of a patient, printing a hollow individual tissue-engineered bone by means of a 3D bio-printing machine; 3) during an operation process of transplanting the artificial bone scaffold, centrifuging blood of the patient to prepare the platelet-rich fibrin, and injecting the platelet-rich fibrin into the tubular cavity in the tissue-engineered bone to obtain the artificial bone which contains the hydroxylapatite, collagen and platelet-rich fibrin and has capability of improving osteanagenesis of human body. The artificial bone can slowly release the autologous growth factor in the platelet-rich fibrin of the patient, thus improving recovery of the osteanagenesis of human body.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a formula of 3D printing artificial bone composite fibrin scaffold and a preparation method thereof. Background technique [0002] The repair of large bone defects has always been a difficult problem for orthopedic surgeons. The current repair strategies include autologous bone, allogeneic bone, and artificial material transplantation. Although autologous bone transplantation is the gold standard of treatment, it will cause bleeding and infection in the bone harvesting area during the transplantation process; there is a risk of immune rejection and disease transmission in allogeneic bone transplantation; the rapid development of bone tissue engineering has greatly reduced the Repair provides a more feasible method. The internal structure of the stent prepared by the traditional method is often uncontrollable, and the 3D printing technology can tailor the shape of th...

AI Technical Summary

Problems solved by technology

Although autologous bone transplantation is the gold standard of treatment, it will cause bleeding and infection in the bone harvesting area during the transplantation process; there is a risk of immune rejection and disease transmission in allogeneic bone transplantation; the rapid development of bone tissue engineering has greatly reduced the Restoration offers a more feasible approach

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