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Steroid saponin compounds and preparation method and application thereof

A technology for steroidal saponins and compounds is applied in the field of steroidal saponins of Polygonatum japonica and their preparation fields, which can solve the problems of no reports on the content determination method, and achieve the effects of being convenient for industrialization and easy to operate.

Active Publication Date: 2018-05-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Though there is the hypoglycemic research report of polygonatum polysaccharide at present, for the research that described steroidal saponins compound is used for treating and improving diabetes or liver cancer, the content assay method of described steroidal saponins compound and take them as evaluation index The assay methods of steroidal saponins are not reported

Method used

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  • Steroid saponin compounds and preparation method and application thereof
  • Steroid saponin compounds and preparation method and application thereof
  • Steroid saponin compounds and preparation method and application thereof

Examples

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preparation example Construction

[0043] The preparation method of the compound is as follows: Polygonatum polygonatum is dried and pulverized at a temperature of -40°C to 60°C, and an aqueous solution of ethanol with a volume concentration of 50% to 70% is used as a mixed solvent, and the mass volume of Polygonatum polygonatum and the mixed solvent is mixed. The ratio is 1:2~20(g / mL) and mixed for ultrasonic extraction, 15℃~60℃ low temperature stirring extraction or reflux extraction; the extract is filtered and concentrated by ultrafiltration membrane and nanofiltration membrane equipment: in membrane concentration equipment The molecular weight cut-off of the membrane is 100-5000, concentrated to 1 / 10-1 / 2 of the volume of the extract; the concentrated solution is adsorbed by a macroporous resin column: macroporous resin column selection: AB-8, D101, D201, D301, HDP100, HDP400, XDA-1, XDA-5, XDA-6, XDA-8, LSA-20, LSA-30, LSA-40, HP-10, HP-20, HP-30, HP-40 or HP-50, Sequentially use water to remove impurities...

Embodiment 1

[0071] Structural formula (1) - purification and preparation of structural formula (8) compound

[0072] Polygonatum odoratum was dried and pulverized at a temperature of 30°C, weighed 150kg, and the aqueous solution of ethanol with a volume concentration of 70% was used as a mixed solvent, and extracted with low-temperature stirring at 30°C (3 times×1000L×2h), and the extract was passed through a membrane Concentration equipment for concentration: first pass ultrafiltration through a membrane system with a molecular weight cut-off of 3000, and then pass through the liquid through a membrane system with a molecular weight cut-off of 500 to obtain a concentrated solution of 200L; the concentrated solution is absorbed by a macroporous resin column HP-20, Sequentially use water, 30% ethanol aqueous solution elution to remove impurities, 70% ethanol aqueous solution elution, collect and combine the eluate; eluent adopts concentration equipment to recover ethanol, and spray-dries to...

Embodiment 2

[0074] Structural formula (1) - purification and preparation of structural formula (8) compound

[0075] Polygonatum odoratum was freeze-dried and pulverized at a temperature of -20°C, weighed 150kg, and used an aqueous solution of ethanol with a volume concentration of 70% as a mixed solvent for reflux extraction (3 times × 1500L × 2h), and the extract was passed through a membrane concentration equipment Concentration: First pass ultrafiltration through a membrane system with a molecular weight cut-off of 2500, and then pass through the liquid through a membrane system with a molecular weight cut-off of 600 to obtain a concentrated solution of 180L; the concentrated solution is absorbed by a macroporous resin column D101, followed by water, 35 After elution with an aqueous solution of ethanol to remove impurities, elution with an aqueous solution of 70% ethanol was carried out, and the combined eluent was collected; the eluent was reclaimed by a concentration device, and spra...

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Abstract

The invention discloses steroid saponin compounds and a preparation method and application thereof. The preparation method comprises the steps of: mixing polygonatum odoratum with an aqueous solutionof ethanol for extraction, performing filtration and concentration by using an ultra filtration membrane and nanofiltration membrane device, then performing adsorption by using a macroporous resin column, performing elution with an aqueous solution of ethanol, performing concentration and recycling of ethanol, and performing freeze drying or spray drying to obtain steroid saponin extract; separating the steroid saponin extract by using low-and-medium pressure liquid chromatography with an OSD column, performing gradient elution by using a methanol aqueous solution with a volume concentration of 10-100%, and performing purification by using a preparative RP-HPLC to obtain the eight steroid saponin compounds. The steroid saponin compounds can be applied to prepare drugs for treating, improving and / or preventing metabolic syndromes, metabolic diseases, metabolic disorder and liver cancers of sugar or fat, food functional additives or healthy food, and the preparation method has simple operation and is easy to industrialize and popularize.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedicine, health products or food additives, and in particular relates to a polygonatum steroidal saponin compound and a preparation method and application thereof. Background technique [0002] Modern medicine believes that diabetes is an endocrine and metabolic disease characterized by glucose metabolism disorder caused by relative or absolute insufficiency of insulin secretion in the body. It is characterized by hyperglycemia and glycosuria, as well as disorders of fat, protein, water and electrolytes, often leading to serious complications, such as nephropathy, eye and neuropathy, coronary heart disease, and cerebrovascular disease. According to statistics, in 2013, there were about 386 million diabetic patients in the world, and about 113 million diabetic patients in my country, and more people were in pre-diabetes. Therefore, the prevention and treatment of diabetes has become a r...

Claims

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Application Information

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IPC IPC(8): C07J71/00C07J9/00A61P3/00A61P3/10A61P13/12A61P3/06A61P35/00A23L33/105G01N30/02
CPCA23L33/105A23V2002/00C07J9/00C07J71/0005G01N30/02A23V2250/21A23V2200/30A23V2200/328A23V2200/3262A23V2200/308A23V2300/14A23V2300/50
Inventor 付红伟张琳
Owner ZHEJIANG UNIV
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