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Method and application for prolonging growth activity of microbial cells

A technology of microbial cells and microbial bacteria, applied in the field of microorganisms, can solve the problems of biological cell growth and decay, and achieve the effect of strengthening stability, good application prospects and slowing down the decay speed.

Inactive Publication Date: 2018-05-18
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adding an appropriate amount of biochar to the soil can improve the physical and chemical properties of the soil, optimize the composition and structure of the soil microbial flora, increase the organic matter content in the soil, and increase crop yields. Therefore, biochar is often used as a new type of soil amendment. Applied to agricultural production, however, the technical problem in the prior art is that there is a lack of a method for using biochar to increase biological activity, so that the growth and activity of biological cells in microbial fertilizers are attenuated

Method used

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  • Method and application for prolonging growth activity of microbial cells

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Experimental program
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Effect test

Embodiment 1

[0019] A method for prolonging microbial cell growth activity, specifically comprising the following steps:

[0020] S1, bacterial suspension preparation

[0021] Inoculate the preserved slant of Bacillus megaterium in 100 ml of LB medium (0.3% beef extract, 0.5% peptone, 0.5% sodium chloride, pH 7.0), and culture it with shaking at 30°C and 120 rpm until the absorbance value (OD 600 ) was 1.0, the Bacillus megaterium microbial bacterial suspension in the stable phase was obtained;

[0022] S2, biochar adsorption

[0023] Take 60 mg of biochar, sterilize it and add it to a 50 ml Erlenmeyer flask containing 10 ml of Bacillus megaterium microbial suspension, shake at room temperature at 120 rpm for 1 hour, and adsorb Bacillus megaterium cells on the biochar , filter, and separate the biochar from the bacterial suspension to obtain the biochar adsorbed with Bacillus megaterium cells.

Embodiment 2

[0025] A method for prolonging microbial cell growth activity, specifically comprising the following steps:

[0026] S1, bacterial suspension preparation

[0027] Inoculate the preserved slant of Bacillus pumilus in 100 ml of LB medium (0.3% beef extract, 0.5% peptone, 0.5% sodium chloride, pH 7.0), shake and cultivate at 30°C and 120 rpm until the absorbance value (OD600) when being 1.0, obtain the bacillus pumilus microorganism seed liquid of stable period;

[0028] Centrifuge the microbial seed solution cultivated to the stable stage under aseptic conditions at 6000 rpm and 4°C for 10 minutes, suspend and mix the bacterial sediment with an appropriate amount of sterile saline to make a suspension of Bacillus pumilus .

[0029] S2, biochar adsorption

[0030] Take 60 mg of biochar, sterilize it and add it into a 50 ml Erlenmeyer flask containing 10 ml of Bacillus pumilus suspension, and vibrate at room temperature at 120 rpm for 1 hour to adsorb the Bacillus pumilus cells...

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Abstract

The invention belongs to the technical field of microbes, and particularly relates to a method and application for prolonging the growth activity of microbial cells. By means of the method, biologicalcharcoal of a porous structure and a microbial bacteria suspension are sufficiently mixed and filtered to obtain biological charcoal with the adsorbed microbial cells. The stability of the microbialcells can be enhanced, the attenuation speed of the growth activity of the microbial cells is reduced, the growth activity of the microbial cells is remarkably prolonged, and the method has good application prospects in the preparation of soil conditioners and microbial bacterial manure.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method and application for prolonging the growth activity of microbial cells. Background technique [0002] Microbial fertilizer is often used as a new type of soil improver or fertilizer in agricultural production, which contains a variety of microorganisms. How to prolong the biological activity of various microorganisms in microbial fertilizer is an important direction of microbial fertilizer research. [0003] Biochar is a kind of extremely insoluble highly aromatic polymer formed after pyrolyzing biomass at a certain temperature under the condition of complete anoxic or partial anoxic, and its carbon content accounts for about 60%-80% %, but also contains other large elements, such as hydrogen, oxygen, nitrogen, sulfur and so on. Porosity and high specific surface area are typical characteristics of biochar, which has strong adsorption capacity and high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C09K17/40C05F11/08C05G3/04C09K101/00C05G3/80
CPCC05F11/08C05G3/80C09K17/40C09K2101/00C12N11/14
Inventor 李宪臻唐文竹
Owner DALIAN POLYTECHNIC UNIVERSITY
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