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Method for fermenting hirsutella sinensis

A Chinese technology of Trichospores and fermentation medium, which is applied in the biological field, can solve problems such as restricting the production efficiency of Trichosporum, low production intensity, and mycelium destruction, so as to improve the utilization rate of substrates and improve the growth environment of bacteria , Slow down the effect of bacterial aging

Active Publication Date: 2021-06-11
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high shear force of the mechanically stirred and ventilated fermenter during use will damage the mycelium and cause the lysis of the bacteria
In addition, batch fermentation requires a lot of labor, high energy consumption, and low production intensity, which seriously restricts the improvement of the production efficiency of China's hairy sp.

Method used

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  • Method for fermenting hirsutella sinensis
  • Method for fermenting hirsutella sinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The Chinese Mortierella strain used in the following examples is Cordyceps sinensis, which has been preserved on October 29, 2020 at the General Microbiology Center of China Microbiological Culture Collection Management Committee, No. 1, Beichen West Road, Chaoyang District, Beijing. Its deposit number is CGMCC No.20759;

[0028] 1. Seed cultivation. Sow the cultured Mortierella chinensis strain seed liquid into the seed medium, the inoculation amount is 10%, and the seed medium is composed of 1% glucose, 0.8% peptone, 0.5% milk powder, 1.5% bran, and 0.3 potassium dihydrogen phosphate. %, 0.3% magnesium sulfate heptahydrate, 0.02% defoamer, and adjust the pH value to 6.0; the culture conditions are: the ventilation ratio is 1:0.5vvm, the tank pressure is 0.03Mpa, the culture temperature is 18-20°C, and it can be obtained by culturing for about 7 hours. seed liquid.

[0029] 2. Fermentation culture. Inoculate the cultivated seed liquid into 60L fermentation medium af...

Embodiment 2

[0031] Basically the same as Example 1. The difference is that the fermenter uses an airlift fermenter. The air-lift fermenter is described in the utility model patent No.: ZL201922236548.7, which is named "an air-lift amino acid fermenter".

[0032] Embodiment 1 is compared with embodiment 2 composition content, and the results are shown in Table 1:

[0033] Table 1

[0034]

[0035] It can be seen from the above table that the average fermentation period of the air-lift fermenter is 23% shorter than that of the mechanical stirring tank; the bacterial cell yield is increased by 9% on average; other indicators are also significantly improved.

Embodiment 3

[0037] 1. Seed cultivation. Sow the cultured Mortierella chinensis strain seed liquid into the seed medium, the inoculation amount is 10%, and the seed medium is composed of 1% glucose, 0.8% peptone, 0.5% milk powder, 1.5% bran, and 0.3 potassium dihydrogen phosphate. %, 0.3% magnesium sulfate heptahydrate, 0.02% defoamer, and adjust the pH value to 6.0; the culture conditions are: the ventilation ratio is 1:0.5vvm, the tank pressure is 0.03Mpa, the culture temperature is 18-20°C, and it can be obtained by culturing for about 7 hours. seed liquid.

[0038] 2. Fermentation culture. The cultured seed solution was inoculated into 50 L of fermentation medium after sterilization, the inoculum amount was 10%, and the fermenter was a 100 L air-lift fermenter. The fermentation medium is composed of 2% glucose, 1.5% corn flour, 1% peptone, 0.5% yeast powder, 2% bran, 0.3% potassium dihydrogen phosphate, 0.3% magnesium sulfate heptahydrate, and 0.03% defoamer; adjust the pH The value...

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Abstract

The invention discloses a method for fermenting hirsutella sinensis, which comprises the following steps: inoculating a seed solution of the hirsutella sinensis into an airlift fermentation tank filled with a fermentation culture medium according to the weight ratio of 10-20%, fermenting and culturing at the temperature of 15-20 DEG C under the conditions that the ventilation ratio is 1: (0.5-1.5) vvm and the tank pressure is 0.02-0.05 Mpa, and feeding and supplementing the culture medium, glucose and hydrogen in the middle and later periods of fermentation. The airlift fermentation tank is adopted to reduce damage of shearing force to mycelia, the thallus growth activity is good, and the reducing sugar metabolism rate is high. On one hand, substrate feedback inhibition is relieved and the delay phase is shortened by adopting a full-nutrition fed-batch mode; on the other hand, nutrient substances are timely supplemented in the middle and later periods of fermentation, and the thallus growth time is prolonged. The utilization rate of the substrate is improved by utilizing glucose online control. Compared with a traditional method, the yield is increased, the mycelium quality is improved, and the content of effective active ingredients in the mycelium is remarkably increased.

Description

technical field [0001] The invention relates to a biological technology, in particular to a method for total nutrient fed-batch and pulse submerged fermentation of Trichosporum chinensis. Background technique [0002] Mortierella chinensis is the asexual generation of Cordyceps sinensis isolated from the stroma and sclerotium of Cordyceps sinensis. There are many physiologically active substances beneficial to the body, such as amino acids, nucleosides, inorganic salts, alkaloids and polysaccharides, etc. in Trichosporum sinensis. It has the functions of regulating the body's immunity, anti-tumor, antibacterial, and anti-fatigue. The mycelium of Mortierella sinensis is cultured by liquid fermentation and then developed into medicines and health care products. [0003] Mortierella chinensis is usually cultured by batch fermentation in a mechanically agitated ventilated fermenter. However, the high shear force of the mechanically stirred and ventilated fermenter during use ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12M1/00C12R1/645
CPCC12N1/14C12M29/06Y02E50/10
Inventor 徐庆阳袁峰王鹏耿燕周帅朱正乔
Owner JIANGSU SHENHUA PHARMA
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