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Preparation and application of denitrifier preparation applicable to low C/N ratio wastewater treatment

A technology for wastewater treatment and denitrification bacteria, applied in the field of environmental engineering, can solve problems such as no application, and achieve the effects of improving denitrification capacity, low cost and high treatment efficiency

Inactive Publication Date: 2018-05-25
ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the quorum sensing effect of signal molecules to improve the activity of denitrification enzymes in activated sludge to prepare denitrification bacteria agents has not been applied in the field of biological denitrification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Inoculate Rhodococcus sp. and Pseudomonas sp. activated on a slant into liquid culture medium, control the temperature at 32°C, aerate at 1:0.5, and culture for 24 to obtain two liquids bacterial liquid;

[0046] (2) Add 3000 mg / L of facultative activated sludge into the artificially prepared sewage, add the liquid bacteria liquid by 1 g / L and add inducer 0.2 mg / L AHLs substances at the same time, carry out interstitial aeration and stirring culture, control Dissolved oxygen concentration 0.2mg / L, temperature control 28°C;

[0047] (3) After cultivating for 3 days, let the sedimentation sludge stand, remove the supernatant, add an equal volume of sewage, and add the two kinds of liquid bacteria liquid and AHLs inducer according to step (2), under the same conditions as step (2) Re-stir and cultivate, and repeat this several times until the sludge concentration reaches 5000mg / L, and the concentration of viable bacteria in the sludge detected by the plate reaches 10 ...

Embodiment 2

[0049] (1) Rhizobium sp. and Comamonas sp. activated by slant. Inoculate them in liquid medium respectively, control the temperature at 35°C, ventilate at 1:0.8, and cultivate for 20 hours to obtain two liquid bacterial solutions;

[0050] (2) Add 4000 mg / L of facultative activated sludge into the artificially prepared sewage, add the liquid bacteria liquid at 2.5 g / L simultaneously and add inducer 0.5 mg / L AHLs substances, and culture with interstitial aeration and stirring, Control the dissolved oxygen concentration to 0.3mg / L, and control the temperature to 30°C;

[0051] (3) After cultivating for 2 days, let the sedimentation sludge stand, remove the supernatant, add an equal volume of sewage, and add the two kinds of liquid bacteria liquid and AHLs inducer according to step (2), under the same conditions as step (2) Re-stir and cultivate, and repeat this several times until the sludge concentration reaches more than 6000mg / L, and the concentration of viable bacteria in t...

Embodiment 3

[0053] (1) Inoculate the Rhodococcus sp. and Rhizobium sp. activated on the slant into the liquid culture medium respectively, control the temperature at 38°C, aerate at 1:1, and cultivate for 12 hours to obtain two liquid bacterial liquids ;

[0054] (2) Add 4000 mg / L of facultative activated sludge into the artificially prepared sewage, and at the same time add the liquid bacteria solution at 5 g / L and add inducer 1 mg / L AHLs substances, and conduct intermittent aeration and stirring culture to control dissolution Oxygen concentration 0.5mg / L, temperature control 35°C;

[0055] (3) After cultivating for 1 day, let the sedimentation sludge stand, remove the supernatant, add an equal volume of sewage, and add the two kinds of liquid bacteria liquid and AHLs inducer according to step (2), under the same conditions as step (2) Re-stir and cultivate, and repeat this several times until the sludge concentration reaches more than 7000mg / L, and the concentration of viable bacteria ...

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Abstract

The invention discloses preparation and application of a denitrifier preparation applicable to low C / N ratio wastewater treatment. A denitrifier preparation applicable to low C / N ratio wastewater treatment is prepared through induction of signal molecules, the signal molecules are N-acyl-homoserine lactone substances (AHLs), and the denitrifier includes heterotrophic nitrification aerobic denitrification bacteria and indigenous fungi. The denitrifier preparation disclosed by the invention is applied to low C / N ratio wastewater treatment, and total nitrogen emission reduction can be achieved. When the denitrifier preparation is put into sewage for treatment, the denitrification efficiency of a biological system is improved and total nitrogen emission reduction is achieved when conventionalprocess conditions are unchanged. After intensified conditioning of the denitrifier preparation disclosed by the invention, the concentration of TN (Total Nitrogen) is reduced by 15% or greater when being compared with that of an original effluent.

Description

technical field [0001] The invention relates to the technical field of environmental engineering, in particular to the preparation and application of a denitrification bacteria preparation suitable for low C / N ratio wastewater treatment. Background technique [0002] The Environmental Statistical Annual Report shows that in 2013, urban sewage treatment plants removed a total of 11.097 million tons of chemical oxygen demand, 1.003 million tons of ammonia nitrogen, and 813,000 tons of total nitrogen. Among industrial enterprises, 18.049 million tons of chemical oxygen demand and 1.096 million tons of ammonia nitrogen have been removed, and the total nitrogen data has not yet been counted. The water quality standard for sewage discharged into urban sewers (CJ 343-2010) gives a total nitrogen limit of 70mg / L, and there are differences in the wastewater discharge standards of various industries. From the overall situation, the total nitrogen emission reduction space of industrial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/01
CPCC02F3/34C02F3/348C12N1/20
Inventor 李明智梅荣武张宇王慧荣王震王晓敏
Owner ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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