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Method for controlling sustained release of cyclodextrin inclusion compound in two-enzyme method

A technology of cyclodextrin inclusion compound and cyclodextrin, which is applied in the fields of food, medicine and textile and clothing, and can solve the problem that the guest molecules cannot be stably and completely utilized.

Inactive Publication Date: 2018-05-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem that the guest molecule in the current cyclodextrin inclusion compound cannot be stabilized, controllable, and fully utilized, and provides a dual-enzyme method to control the sustained release of the cyclodextrin inclusion compound, so that the guest molecule Molecules are released uniformly, at a controlled rate, thoroughly and stably

Method used

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  • Method for controlling sustained release of cyclodextrin inclusion compound in two-enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 The sodium benzoate-β-cyclodextrin inclusion complex is released uniformly, slowly and completely under the action of dual enzymes

[0024] (1) Dissolve 1.14 g (0.001 mol) of β-cyclodextrin in 100 mL of water, add 0.15 g (0.001 mol) of sodium benzoate to the aqueous solution of β-cyclodextrin, and stir at room temperature for 12 hours;

[0025] (2) Freeze-dry the solution obtained in step (1) at -50°C and 10Pa vacuum for 48 hours to obtain a mixture of sodium benzoate-β-cyclodextrin inclusion compound, and wash the powder with -4°C ultrapure water 3 times, until there is no sodium benzoate in the cold water;

[0026] (3) drying the solution in step (2) at 45°C to obtain sodium benzoate-β-cyclodextrin inclusion compound;

[0027] (4) dissolving the sodium benzoate-β-cyclodextrin inclusion compound in step (3) in a phosphate buffer solution with a pH of 5.5 to prepare a 1% reaction substrate solution;

[0028] (5) Take 900 μL of the solution from step (4) in a...

Embodiment 2

[0029] Example 2: Uniform, slow and complete release of vanillin-β-cyclodextrin inclusion compound under the action of dual enzymes

[0030] (1) Dissolve 1.14 g of β-cyclodextrin in 100 mL of water, add 0.15 g of vanillin to the aqueous solution of β-cyclodextrin, and stir for 12 hours at room temperature;

[0031] (2) Freeze-dry the solution obtained in step (1) at -50°C and 30Pa vacuum for 24 hours to obtain a mixture of vanillin-β-cyclodextrin inclusion compound, wash the powder 3 times with ethanol, and put it in ethanol No vanillin present;

[0032] (3) drying the step (2) at 25°C to obtain the inclusion compound of vanillin-β-cyclodextrin;

[0033] (4) Dissolve the powder of step (3) in a phosphate buffer solution with a pH of 5.5 to make a 1% reaction substrate solution;

[0034] (5) Take 900 μL of the solution from step (4) in a centrifuge tube, add 200 μL CGTase and 200 μL glucoamylase for reaction, and the guest molecule vanillin will be released uniformly and comp...

Embodiment 3

[0035] Example 3: Uniform, slow and complete release of β-carotene-γ-cyclodextrin inclusion compound under the action of dual enzymes

[0036] (1) Dissolve 2.27 g of γ-cyclodextrin in 100 mL of water, add 0.53 g of β-carotene n-hexane solution to the aqueous solution of γ-cyclodextrin, and stir for 12 hours at room temperature in the dark;

[0037] (2) Freeze-dry the liquid obtained in step (1) at -50°C and 20Pa vacuum for 36 hours to obtain a mixture of β-carotene-γ-cyclodextrin inclusion compound, wash the powder 3 times with ethanol, and put it in ethanol No β-carotene present;

[0038] (3) drying the solution in step (2) in the dark at 30°C to prepare β-carotene-γ-cyclodextrin inclusion compound;

[0039] (4) Dissolve the powder of step (3) in a phosphate buffer solution with a pH of 5.5 to make a 1% reaction substrate solution;

[0040] (5) Take 900 μL of the solution from step (4) in a centrifuge tube, add 10 μL CGTase and 10 μL glucoamylase to react, and the guest mol...

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Abstract

The invention relates to a method for controlling the sustained release of a cyclodextrin inclusion compound in a two-enzyme method, which belongs to the technical field of food, drugs, textile and clothing. Under the joint effect of cyclodextrin glycosyltransferase and glucoamylase, and under the condition that the object molecular release speed is controllable, the object molecules in the inclusion compound can be gradually stably released from the cavity of the cyclodextrin, so that the object molecules of the inclusion compound can be maximally utilized. The method provided by the invention can constantly and steadily release the object molecules (essence, spice and pigment) in the inclusion compound in a uniform and thorough manner and with a controllable speed, rather than release inone time, so that the object molecules can be maximally utilized. The release speed can be adjusted according to the release requirement of the object molecules of the inclusion compound and the drugs can be uniformly and thoroughly released with a controllable speed.

Description

technical field [0001] The invention relates to a method for controlling the sustained release of cyclodextrin inclusion complexes by a dual-enzyme method, in particular to a uniform, speed-controllable and thorough release of cyclodextrin inclusion complexes under the joint action of cyclodextrin glycosyltransferase and glucoamylase , to improve the maximum utilization rate of guest molecules, belonging to the technical fields of food, medicine and textile and clothing. Background technique [0002] Cyclodextrins are a type of cyclic maltoligosaccharides formed by linking D-glucopyranose units through α-(1→4)-glycosidic bonds. According to the number of glucose units (6-13), cyclodextrin can be divided into α-, β-, γ-, δ-,······θ-cyclodextrin and so on. In addition to the above parent cyclodextrins, functional group modified cyclodextrins, cyclodextrin dimers and cyclodextrin polymers have appeared. Cyclodextrin is a non-reducing cyclic oligosaccharide, which is obtained ...

Claims

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Application Information

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IPC IPC(8): C12P19/18C12P19/04C08B37/16A61K47/69
CPCC08B37/0015C12P19/04C12P19/18
Inventor 柏玉香金征宇夏柳溪吴云周星王金鹏田耀旗焦爱权赵建伟谢正军徐学明
Owner JIANGNAN UNIV
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