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Exosome-compounded collagen biological scaffold with directional releasing function as well as preparation method and application thereof

A collagen biology and exosome technology, applied in the field of medical biomaterials, can solve the problems of difficulty in forming a target, and MSC-Exo cannot be administered in a concentrated area, so as to achieve effective retention of activity and improve targeted repair and therapeutic effects. , the preparation method is simple and easy to implement

Active Publication Date: 2018-01-09
龙乾发 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still many problems in the use of MSC-Exo in the treatment of diseases. The most important point is that the existing methods of drug delivery, such as intravenous injection, intranasal administration, and intracerebroventricular administration, are easy to spread, resulting in the inability of MSC-Exo to concentrate on the site. Therefore, it is urgent to construct a drug-loading system that can realize the targeted / directed therapeutic effect of MSC-Exo, so as to give full play to the potential wide range of therapeutic uses of MSC-Exo

Method used

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  • Exosome-compounded collagen biological scaffold with directional releasing function as well as preparation method and application thereof
  • Exosome-compounded collagen biological scaffold with directional releasing function as well as preparation method and application thereof
  • Exosome-compounded collagen biological scaffold with directional releasing function as well as preparation method and application thereof

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Embodiment Construction

[0032] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0033] 1. Main reagents and instruments

[0034] Pepsin, Chloroform, Glacial Acetic Acid, NaHCO 3 , NaCl, high-speed centrifuge, freeze dryer, deep-low temperature refrigerator, meat grinder, dialysis membrane, liquid nitrogen planetary ball mill.

[0035] 2. Method

[0036] 2.1 Collagen Preparation

[0037] (1) Pretreatment: take fresh bovine (pig, sheep or other animal) Achilles tendon, bovine (pig, sheep or other animal) skin, bovine (pig, sheep or other animal) articular cartilage and other biological materials (for example, take skin), wash and remove subcutaneous fat and dermis, remove fascia, remove hair, and chop finely.

[0038] (2) Degreasing: The shredded biological material is washed with double distilled water, minced in a meat grinder, soaked in a mixed solvent of chloroform-methanol (for example, volume ratio 1:1) at 2-6°C (...

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Abstract

The invention provides an exosome-compounded collagen biological scaffold with a directional releasing function as well as a preparation method and application thereof. The exosome-compounded collagenbiological scaffold comprises nano collagen and exosomes dispersed in the nano-collagen. The preparation method of the biological scaffold comprises the following steps: separating collagen from animal tissue, carrying out ball milling till the collagen is nanoscale collagen; separating exosomes from culture of bone marrow or umbilical cord mesenchymal stem cells; mixing the exosomes with the collagen, freezing and drying. The preparation method of the biological scaffold is used for dispersing the exosomes in the nanoscale collagen to form a biological scaffold material with a function of directionally releasing exosomes; the targeted repairing and treating effects of the exosomes can be obviously improved; the development tendency of accurate drug administration can be met; the dosage forms are various; the biological scaffold has extensive clinical application values.

Description

technical field [0001] The invention belongs to the field of medical biomaterials, in particular to an exosome-composite collagen biological scaffold. Background technique [0002] Domestic and foreign studies have confirmed that bone marrow or umbilical cord mesenchymal stem cell exosomes (MSC-derived exosomes, MSC-Exo) have obvious immune regulation and neuroprotective functions. Compared with simple cell therapy, MSC-Exo is easier to penetrate the skin, Mucous membrane, nerve sheath, fascia, blood-brain barrier and post-traumatic damaged tissue can avoid the risk of cell tumorigenesis, and it is convenient for freeze-thaw storage. It is comprehensively suggested that MSC-Exo can become a more ideal clinical bioremediation agent. However, there are still many problems in the use of MSC-Exo in the treatment of diseases. The most important point is that the existing methods of drug delivery, such as intravenous injection, intranasal administration, and intracerebroventricula...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K47/42A61P25/00A61P17/00A61P19/04A61P25/08A61P25/28A61P9/10
Inventor 龙乾发李征宇
Owner 龙乾发
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