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Gelatin cell scaffold with controllable porous structure and preparation method thereof

A cell scaffold and pore structure technology, applied in the field of gelatin cell scaffold and its preparation, can solve the problems of uncontrollable size and uneven pore size of materials, and achieve the effects of strong mechanical properties and excellent biological properties.

Inactive Publication Date: 2018-06-08
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of uneven material pore size and uncontrollable size of current commercial cell scaffolds, the present invention provides a gelatin cell scaffold with controllable pore structure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A gelatin aqueous solution with a mass concentration of 20% was prepared as the dispersed phase, and a liquid paraffin containing sorbitan oleate with a mass concentration of 3% was prepared as the continuous phase. Add 1 part by volume of the dispersed phase to 4 parts by volume of the continuous phase, and emulsify at 50° C. for 30 min under mechanical stirring at 300 rpm to obtain a uniform emulsion. Add 1% dopamine to the above emulsion, which accounts for 1% of the dry weight of gelatin, and react at 50°C for 80 minutes under mechanical stirring at 300 rpm. Then add dialdehyde carboxymethyl cellulose accounting for 2% of the dry weight of gelatin to the above emulsion, and react at 50°C for 5 minutes under mechanical stirring at 300 rpm. Stop stirring and centrifuge the emulsion to collect gelatin microspheres, react at 25°C for 16 hours to make the microspheres adhere to each other to form a gel, and then use petroleum ether and pure water to wash the gel alternat...

Embodiment 2

[0023] An aqueous gelatin solution with a mass concentration of 25% was prepared as the dispersed phase, and sunflower oil containing sorbitan monostearate with a mass concentration of 5% was prepared as the continuous phase. Add 1 part by volume of the dispersed phase to 6 parts by volume of the continuous phase, and emulsify at 60° C. for 30 min under mechanical stirring at 500 rpm to obtain a uniform emulsion. Add 0.5% of genipin to the above emulsion, accounting for the dry weight of gelatin, and react at 60°C for 60 minutes under mechanical stirring at 500 rpm. Then add dialdehyde xanthan gum accounting for 5% of the dry weight of gelatin to the above emulsion, and react at 60° C. with mechanical stirring at 500 rpm for 3 minutes. Stop stirring and then centrifuge the emulsion to collect gelatin microspheres, react at 15°C for 24 hours to make the microspheres adhere to each other to form a gel, and then use petroleum ether and pure water to wash the gel alternately to re...

Embodiment 3

[0025] An aqueous gelatin solution with a mass concentration of 30% was prepared as the dispersed phase, and isopropyl myristate containing polyglycerol ricinoleate with a mass concentration of 8% was prepared as the continuous phase. Add 1 part by volume of the dispersed phase to 8 parts by volume of the continuous phase, and emulsify at 70° C. for 30 min under mechanical stirring at 700 rpm to obtain a uniform emulsion. Add 1.5% tannic acid to the above emulsion, and react at 70°C and 700 rpm for 45 minutes under mechanical stirring at 700 rpm. Then add dialdehyde starch accounting for 8% of the dry weight of gelatin to the above emulsion, and react at 70°C for 1 min under mechanical stirring at 700 rpm. Stop stirring, then let the emulsion stand still to break the emulsion, collect the gelatin microspheres, react at 10°C for 36 hours to make the microspheres stick to each other to form a gel, and then use petroleum ether and pure water to wash the gel alternately to remove ...

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Abstract

The invention provides a gelatin cell scaffold with a controllable porous structure and a preparation method thereof. The preparation method comprises the following steps: firstly, preparing uniform latex emulsion through an emulsion process; secondly, adding a cross-linking agent into the latex emulsion to prepare gelatin microspheres; thirdly, bonding the gelatin microspheres in a crosslinking manner in a demulsification process to form gel; lastly, performing freeze drying on the gel to obtain the gelatin cell scaffold. The gelatin cell scaffold with the controllable porous structure is prepared by bonding the gelatin microspheres of different particle sizes, so that the problem that the conventional cell scaffold is non-uniform in particle size and is uncontrollable in size is solved effectively. The gelatin cell scaffold provided by the invention has high biocompatibility, biodegradability and higher mechanical performance. Moreover, the preparation method is green, is environmentally friendly, is easy to operate, and is feasible. Thus, the gelatin cell scaffold provided by the invention has a good market application prospect in the field of tissue engineering.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a gelatin cell scaffold with good biocompatibility, biodegradability, uniform pore size and controllable size and a preparation method thereof. Background technique [0002] Tissue engineering is the application of cell biology, biomaterials and engineering principles to develop biological substitutes to repair or reconstruct tissue or organ function. Seed cells, growth factors and cell scaffolds are the three major elements of tissue engineering. Among them, cell scaffold is a very important component in tissue engineering. Scaffolds can provide an ideal environment for cell attachment, migration, and proliferation, and guide their growth, eventually forming the desired three-dimensional tissue structure. [0003] Materials used as cell scaffolds should have high porosity, high specific surface area, and interpenetrating geometry as well as unique three-dimensional shapes. T...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/20A61L27/50A61L27/56
CPCA61L27/20A61L27/222A61L27/50A61L27/56C08L89/00
Inventor 李德富袁伦穆畅道葛黎明刘诗雨
Owner SICHUAN UNIV
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