Gelatin cell scaffold with controllable porous structure and preparation method thereof
A cell scaffold and pore structure technology, applied in the field of gelatin cell scaffold and its preparation, can solve the problems of uncontrollable size and uneven pore size of materials, and achieve the effects of strong mechanical properties and excellent biological properties.
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Embodiment 1
[0021] A gelatin aqueous solution with a mass concentration of 20% was prepared as the dispersed phase, and a liquid paraffin containing sorbitan oleate with a mass concentration of 3% was prepared as the continuous phase. Add 1 part by volume of the dispersed phase to 4 parts by volume of the continuous phase, and emulsify at 50° C. for 30 min under mechanical stirring at 300 rpm to obtain a uniform emulsion. Add 1% dopamine to the above emulsion, which accounts for 1% of the dry weight of gelatin, and react at 50°C for 80 minutes under mechanical stirring at 300 rpm. Then add dialdehyde carboxymethyl cellulose accounting for 2% of the dry weight of gelatin to the above emulsion, and react at 50°C for 5 minutes under mechanical stirring at 300 rpm. Stop stirring and centrifuge the emulsion to collect gelatin microspheres, react at 25°C for 16 hours to make the microspheres adhere to each other to form a gel, and then use petroleum ether and pure water to wash the gel alternat...
Embodiment 2
[0023] An aqueous gelatin solution with a mass concentration of 25% was prepared as the dispersed phase, and sunflower oil containing sorbitan monostearate with a mass concentration of 5% was prepared as the continuous phase. Add 1 part by volume of the dispersed phase to 6 parts by volume of the continuous phase, and emulsify at 60° C. for 30 min under mechanical stirring at 500 rpm to obtain a uniform emulsion. Add 0.5% of genipin to the above emulsion, accounting for the dry weight of gelatin, and react at 60°C for 60 minutes under mechanical stirring at 500 rpm. Then add dialdehyde xanthan gum accounting for 5% of the dry weight of gelatin to the above emulsion, and react at 60° C. with mechanical stirring at 500 rpm for 3 minutes. Stop stirring and then centrifuge the emulsion to collect gelatin microspheres, react at 15°C for 24 hours to make the microspheres adhere to each other to form a gel, and then use petroleum ether and pure water to wash the gel alternately to re...
Embodiment 3
[0025] An aqueous gelatin solution with a mass concentration of 30% was prepared as the dispersed phase, and isopropyl myristate containing polyglycerol ricinoleate with a mass concentration of 8% was prepared as the continuous phase. Add 1 part by volume of the dispersed phase to 8 parts by volume of the continuous phase, and emulsify at 70° C. for 30 min under mechanical stirring at 700 rpm to obtain a uniform emulsion. Add 1.5% tannic acid to the above emulsion, and react at 70°C and 700 rpm for 45 minutes under mechanical stirring at 700 rpm. Then add dialdehyde starch accounting for 8% of the dry weight of gelatin to the above emulsion, and react at 70°C for 1 min under mechanical stirring at 700 rpm. Stop stirring, then let the emulsion stand still to break the emulsion, collect the gelatin microspheres, react at 10°C for 36 hours to make the microspheres stick to each other to form a gel, and then use petroleum ether and pure water to wash the gel alternately to remove ...
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