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African swine fever virus multi-epitope fusion diagnostic antigen and its preparation method and use

An African swine fever virus and multi-epitope technology, applied in the biological field, can solve the problems of low specificity and low sensitivity, and achieve the effect of high sensitivity and strong specificity

Inactive Publication Date: 2020-05-05
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have shown that cell-cultured whole virus antigens have high sensitivity but low specificity; single recombinant protein antigens have high specificity but low sensitivity

Method used

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  • African swine fever virus multi-epitope fusion diagnostic antigen and its preparation method and use
  • African swine fever virus multi-epitope fusion diagnostic antigen and its preparation method and use
  • African swine fever virus multi-epitope fusion diagnostic antigen and its preparation method and use

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0066] The present invention will be further described in detail below through specific examples.

[0067] 1. Prediction and analysis of dominant antigenic epitopes of important antigenic genes of ASFV.

[0068] According to the whole genome sequence of ASFV (GenBank accession number: U18466.2), using biological online software ABCPred (http: / / www.imtech.res.in / raghava / abcpred / ), BepiPred 1.0 Server (http: / / www.cbs .dtu.dk / services / BepiPred / ), Immunomedicine Group (http: / / imed.med.ucm.es / Tools / antigenic.pl) and DNAStar-Protean software on ASFV important antigen genes (P72, P54, P30 and P12 ) to predict and analyze the epitope of the amino acid sequence encoded by ), and find the sequence with common dominant linear epitope by combining four different prediction methods (Table 1).

[0069] 2. Construction of ASFV multi-epitope fusion antigen gene.

[0070] Through the nucleotide sequence (GGCCCGGGC) encoding flexible amino acid (GPG), the genes encoding dominant antigenic epi...

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Abstract

The invention discloses a multi-epitope fusion diagnosis antigen for African swine fever virus as well as a preparation method and application thereof. An ASFV (African swine fever virus) important structural protein gene encoding amino acid sequence is analyzed, screened and recombined through bioinformatics software, a multi-epitope fusion antigen gene is built and synthesized and is expressed in bacillus coli; through screening, the recombinant multi-epitope fusion antigen ASFV-meAg6 is obtained, so that diagnosis antigen protein with strong specificity and high sensitivity is provided foran ASFV serological diagnosis method.

Description

technical field [0001] The invention relates to an African swine fever virus (ASFV) multi-epitope fusion diagnostic antigen ASFV-meAg6, a preparation method and application thereof, and belongs to the field of biotechnology. Background technique [0002] African swine fever (ASF) is an acute, febrile, highly contagious infectious disease of pigs caused by African swine fever virus (ASFV). The virus can infect pigs of all breeds and ages. Clinically, sick pigs are characterized by high fever, loss of appetite, skin and internal organ bleeding, and the mortality rate is as high as 100%. [0003] The disease is listed as a first-class animal disease in my country, and belongs to the legally notifiable animal disease of the World Organization for Animal Health (OIE). ASF was initially prevalent mainly in Africa and eastern Europe, and was later found outside Africa in countries such as Sardinia, Spain, Portugal, the Caribbean, Brazil, and Eastern Europe. In 2007, the disease c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/70G01N33/569
CPCC07K14/005C07K2319/40C12N15/62C12N15/70C12N2710/12022C12N2800/22G01N33/56983G01N2469/20
Inventor 乔军孟庆玲郭晶李杰乔梦凡张星星李重阳孟丹伍晔晖王熙凤贡莎莎蔡扩军
Owner SHIHEZI UNIVERSITY