A molecularly imprinted magnetic nanoparticle that specifically adsorbs bovine hemoglobin

A technology of magnetic nanoparticles and bovine hemoglobin, which is applied in other chemical processes, chemical instruments and methods, alkali metal compounds, etc., can solve the problems of low separation efficiency and complicated sedimentation process, and achieve simple and fast reaction and mild adsorption and separation conditions Effect

Active Publication Date: 2019-10-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These surface molecular imprinting materials all have the characteristics of high imprinting efficiency, but most of them have low separation efficiency due to the presence of hydrophilic groups and complicated sedimentation process

Method used

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  • A molecularly imprinted magnetic nanoparticle that specifically adsorbs bovine hemoglobin
  • A molecularly imprinted magnetic nanoparticle that specifically adsorbs bovine hemoglobin
  • A molecularly imprinted magnetic nanoparticle that specifically adsorbs bovine hemoglobin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 No Fe 3 o 4 Silanized Acrylic Functionalization of Magnetic Nanoparticles

[0035] Weigh FeSO 4 ·7H 2 O (0.8008g) and FeCl 3 ·6H 2 O (1.1677g) was placed in a four-neck flask, 200mL of deionized water and 40mL of absolute ethanol were added, and nitrogen gas was introduced. The reaction system was placed in a water bath environment at 60° C., and stirred with a mechanical stirrer at a speed of 900 r / min for 0.5 h to remove oxygen in the reaction system. Subsequently, 5 mL of 25% ammonia water was added to make the pH of the system 10-11, and the solution instantly changed from brownish red to black, and the solution was aged for 10 minutes and then vacuum-dried.

[0036] Take 60mg of Fe produced 3 o 4 Particles, sequentially 70 μL functional monomer methacrylic acid (MAA) and 25 mg itaconic acid (IA), 16 mg crosslinker methylenebisacrylamide (N'N-MBA), and 20 mL PBS buffer solution (0.02M, pH7 .00), ultrasonically oscillating and mixing for 10 minutes...

Embodiment 2

[0040] Example 2 No Fe 3 o 4 Acrylic functionalization modification of magnetic nanoparticles

[0041] Weigh FeSO 4 ·7H 2 O (0.8008g) and FeCl 3 ·6H 2 O (1.1677g) was placed in a four-neck flask, 200mL of deionized water and 40mL of absolute ethanol were added, and nitrogen gas was introduced. The reaction system was placed in a water bath environment at 60° C., and stirred with a mechanical stirrer at a speed of 900 r / min for 0.5 h to remove oxygen in the reaction system. Then 5 mL of 25% ammonia water was added to make the pH of the system 10-11, the solution changed from brownish red to black instantly, and the reaction was matured for 10 minutes.

[0042] Lower the temperature of the mature reaction system to 30°C, add 20 mL of 5% TEOS ethanol solution dropwise for silanization modification, keep mechanical stirring at 900r / min, and react for 2 hours to obtain silanization-modified magnetic particles Fe 3 o 4 @SiO 2 . Place the system in an external magnetic fiel...

Embodiment 3

[0048] Weigh FeSO 4 ·7H 2 O (0.8008g) and FeCl 3 ·6H 2 O (1.1677g) was placed in a four-neck flask, 200mL of deionized water and 40mL of absolute ethanol were added, and nitrogen gas was introduced. The reaction system was placed in a water bath environment at 60° C., and stirred with a mechanical stirrer at a speed of 900 r / min for 0.5 h to remove oxygen in the reaction system. Then 5 mL of 25% ammonia water was added to make the pH of the system 10-11, the solution changed from brownish red to black instantly, and the reaction was matured for 10 minutes.

[0049] The temperature of the matured reaction system was lowered to 30° C., 20 mL of 5% TEOS ethanol solution was added dropwise for silanization modification, and mechanical stirring was maintained at 900 r / min for 2 hours of reaction.

[0050] Place the silanized modified reaction solution in an external magnetic field, remove the supernatant reaction solution, wash the magnetic particles several times with deionize...

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Abstract

The invention discloses molecular imprinted magnetic nanoparticle capable of specifically adsorbing bovine hemoglobin, which belong to the technical field of biochemical separation. The molecular imprinted functionalized magnetic nanoparticles having the capability of specifically adsorbing a target protein can be prepared, the adsorption and separation conditions for bovine hemoglobin are mild bythe nanoparticles, the reaction is simple and rapid, and the specific adsorption of the target protein-bovine hemoglobin can be achieved in a protein mixed solution, which lays a good foundation forthe separation and purification of proteins.

Description

technical field [0001] The invention relates to a molecularly imprinted magnetic nanoparticle that specifically adsorbs bovine hemoglobin, and belongs to the technical field of biochemical separation. Background technique [0002] Molecular imprinting is a phenomenon in which a substrate and a target molecule are complementary in terms of molecular size, conformation, three-dimensional structure, and active groups. Molecularly Imprinted Technology (MIT) uses target molecules as templates, combines functionalized polymer monomers with a complementary structure to the target molecules and template molecules, and adds a cross-linking agent for polymerization reaction. After the reaction is completed, the template molecules are washed. Take off to form a molecularly imprinted polymer with a fixed hole size and shape and a defined arrangement of functional groups. Molecular imprinting technology is most widely used in the identification and separation of small molecules, such as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F292/00C08F220/06C08F222/02C08F222/38C08J9/26B01J20/26B01J20/28
CPCB01J20/261B01J20/28009B01J20/28016C08F292/00C08J9/26C08J2351/10C08F220/06C08F222/02C08F222/38
Inventor 苏宇杰邱碧霞杨严俊
Owner JIANGNAN UNIV
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