Kit and method for measuring content of squamous cell carcinoma antigen

A technology of squamous epithelium and antigen content, applied in the field of biochemistry, can solve the problems of cumbersome determination steps and expensive reagents, and achieve the effects of shortened reaction time, expanded detection range and high accuracy

Inactive Publication Date: 2018-06-15
JIANGSU ZECEN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects of cumbersome determination steps and expensive reagents in the existing squamous cell carcinoma antigen determination method, and provide a highly accurate method for the determination of squamous epithelial cells in human serum by magnetic particle chemiluminescence. Kit for Cell Carcinoma Antigen (SCC) Assay

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  • Kit and method for measuring content of squamous cell carcinoma antigen

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Embodiment Construction

[0042] Preferred embodiments of the present invention are described below, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0043] The operation procedure of adopting the test kit test sample of the present invention is as follows:

[0044] 1. Sample collection

[0045] Use correct medical techniques to collect serum (severe hemolysis or lipemia samples cannot be used for determination), and the collected samples should not be kept at room temperature for more than 8 hours; if not tested within 8 hours, the samples should be placed in a refrigerator at 2-8°C ; If it needs to be stored or transported for more than 72 hours, it should be frozen at below -20°C and avoid repeated freezing and thawing. Return to room temperature before use, shake gently to mix.

[0046] 2. Preparation before experiment

[0047] ① Take a bottle of lotion a...

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Abstract

The invention discloses a kit for measuring the content of a squamous cell carcinoma antigen (SCC) in human serum by a magnetic particle chemiluminescence method. The kit comprises a calibrator, a quality control, a resistant reagent, a magnetic particle reagent and a luminescent substrate. The resistant reagent comprises fluorescein isothiocyanate-labeled squamous cell carcinoma antigen-coated antibodies and alkaline phosphatase-labeled squamous cell carcinoma antigen-coated antibodies. The magnetic particle reagent is a connector of magnetic particles and goat anti-FITC. The magnetic particle is used as an immune reaction solid phase. Through combination of a chemiluminescence enzyme-linked immunoassay method and a chemiluminescence measuring instrument, the method is used for detectingthe SCC content of human serum, simultaneously measures multiple samples based on a fully automated chemiluminometer, realizes high-throughput rapid measurement of SCC, and greatly improves accuracy,specificity, precision and detection efficiency.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring the content of squamous cell carcinoma antigen (SCC) in human body by using magnetic particle chemiluminescence method. Background technique [0002] Squamous cell carcinoma antigens are a group of glycoproteins belonging to the serine / cysteine ​​protease inhibitor family with a molecular weight of 45,000 Daltons. SCC was originally isolated from squamous cell carcinoma tissues by Kato et al., and consists of at least 10 subunits with different isoelectric points. [0003] As a tumor marker of squamous cell carcinoma, squamous cell carcinoma antigen can be used to detect cervical cancer, vulvar cancer, lung cancer, esophageal cancer and other diseases. The level of SCC in the serum of cervical cancer before treatment can be used as an early prognosis indicator, and adjuvant treatment should be given to the screened high-risk patients. More importantly, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/543G01N21/76
CPCG01N33/57484G01N21/76G01N33/54326
Inventor 汪丹刘振世薛黎金鑫徐茜茜
Owner JIANGSU ZECEN BIOTECH CO LTD
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