A kind of compound culture medium for cultivating Spirulina platensis

A technology of spirulina platensis and culture medium, applied in the direction of microorganism-based methods, microorganisms, bacteria, etc., can solve the problems of slow growth of algae cells and high cost of culture medium of spirulina platensis, so as to improve corporate profits and increase industrial added value Value, comprehensive effect of nutrients

Active Publication Date: 2020-12-18
鄂托克旗哈马太微藻养殖技术咨询有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve defects such as high cost of Spirulina platensis culture medium and slow growth of algae cells in the prior art, and provide a composite medium for cultivating Spirulina platensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of compound medium for cultivating Spirulina platensis, it comprises following raw material, according to 1L formula:

[0023] Sodium nitrate 0.3g, sodium bicarbonate 0.5g, sodium chloride 0.1g, ferric ammonium citrate 0.07g, sodium molybdate 0.02g, zinc sulfate 0.01g, propionamide 0.001g, wheat bran extract 10ml, rice straw extract 30ml, the balance is water, adjust the pH to 9, and dilute to 1L;

[0024] The wheat bran extract is prepared according to the following process:

[0025] Grind the wheat bran and place it in a reaction tank, add 2 times the weight of 6M hydrochloric acid, stir and hydrolyze at 60°C for 8 hours at a stirring speed of 100rpm, collect the filtrate by filtration, and then use 2M sodium hydroxide solution and residual hydrochloric acid, adjust the pH of the solution to be 9, to obtain final product;

[0026] The rice straw extract is prepared according to the following process:

[0027] Put the rice straw in a pulverizer to crush it, t...

Embodiment 2

[0029] A kind of compound medium for cultivating Spirulina platensis, it comprises following raw material, according to 1L formula:

[0030] Sodium nitrate 0.5g, sodium bicarbonate 0.3g, sodium chloride 0.2g, ferric ammonium citrate 0.05g, sodium molybdate 0.03g, zinc sulfate 0.02g, propionamide 0.002g, wheat bran extract 20ml, rice straw extract 20ml, the balance is water, adjust the pH to 8, and dilute to 1L;

[0031] The wheat bran extract is prepared according to the following process:

[0032] Grind the wheat bran and place it in a reaction tank, add 2 times the weight of 6M hydrochloric acid, stir and hydrolyze at 60°C for 8 hours at a stirring speed of 100rpm, collect the filtrate by filtration, and then use 2M sodium hydroxide solution and residual hydrochloric acid, adjust the pH of the solution to be 8, to obtain final product;

[0033] The rice straw extract is prepared according to the following process:

[0034] Put the rice straw in a pulverizer to crush it, t...

Embodiment 3

[0036] Composite medium of the present invention cultivates Spirulina platensis effect test:

[0037]Spirulina platensis in the logarithmic growth phase was inoculated into the culture medium for cultivation, the light intensity was controlled at 4000 lux, the temperature was controlled at 32° C., and the air flow rate was 0.4 vvm.

[0038] The experimental group is Example 1; the control group 1 uses Zaruk culture medium; the control group 2 does not add wheat bran extract, and the rest is the same as in Example 1; the control group 3 does not add rice straw extract, and the rest is the same as in Example 1 ; The remaining culture conditions are the same as above, and the experimental group and the control group 1-3 are the same. The cell density (OD560) of Spirulina platensis in the medium was detected by a spectrophotometer every other day, and the density on day 0 was the density at the time of inoculation; see Table 1 for details:

[0039] Table 1

[0040] numb...

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PUM

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Abstract

The invention belongs to the technical field of alga cultivation, and discloses a composite medium for cultivating Spirulina platensis. The composite medium comprises sodium nitrate, sodium bicarbonate, sodium chloride, ammonium ferric citrate, sodium molybdate, zinc sulfate, propionamide, wheat bran extract and rice straw extract. The medium uses a large amount of agricultural wastes, and the quantity of raw material components is small, so the cost is saved.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a compound culture medium for cultivating spirulina platensis. Background technique [0002] Spirulina, also known as "arthrospira". Cyanobacteria, Oscillatoriaceae. The algal body is an unbranched filament composed of a single row of cells. The colloidal sheath has no or only a very thin sheath, and has a regular spiral shape to form algal growth stages. No heterocyst and posterior wall spores. About 38 species, most of which grow in alkaline salt lakes. At present, there are large-scale artificial cultivation at home and abroad, mainly three kinds of Spirulina platensis, Spirulina maxima and Spirulina indica. Edible, nutritious, protein content as high as 60%-70%. In natural waters, its massive reproduction will form algal blooms. The optimal growth pH range of Spirulina is 3-11.0, and when the pH is higher than 11.0, it will be unfavorable for growt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 吕兰婷陈金华吕伟德黄土根陈国汀陈国华
Owner 鄂托克旗哈马太微藻养殖技术咨询有限公司
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