Method for purifying flavonoid quercetin from guava leaves

Abstract

The invention discloses a method for purifying flavonoid quercetin from guava leaves. The method comprises the following steps: (1) pulverizing the guava leaves, adding water, heating and filtering toobtain a guava extract flavone crude product; (2) dissolving into an absolute ethanol solution, filtering, taking the supernatant and performing ultrafiltation circulation to obtain an ultrafiltration film filtering solution; (3) dissolving a quercetin monomer serving as a template molecule into acetonitrile and adding a functional monomer 4-vinylpyron to prepare a molecular imprinting polymer; and (4) adding the ultrafiltration film filtering solution into the molecular imprinting polymer, performing airtight oscillation in a constant-temperature shaking table and performing centrifugation to obtain the flavonoid quercetin. The material liquid separated by the purification method provided by the invention is further refined and purified by the synthesized imprinting polymer, the purifiedguava leaf flavonoid quercetin is obtained, the process is simple and environment-friendly, the cost is low, the income is high and the purification effect is good.

Description

technical field [0001] The invention belongs to the field of purifying guava leaf compounds, in particular to a method for purifying the flavonoid quercetin in guava leaves. Background technique [0002] Guava (Psidium guajava Linn.) Myrtaceae tree, native to South America. Cultivated in various places in South China, wild species are common, and it reaches the Anning Valley in the southwestern part of Sichuan in the north. It grows in wasteland or low hills; the fruit is edible; the leaves contain volatile oil and tannin, etc., which are used medicinally to stop dysentery and stop bleeding. , invigorating the stomach and other effects; the leaves are boiled to remove tannin, dried for tea, sweet in taste, and has heat-clearing effect. Studies on the chemical composition of guava leaves show that: guava leaves contain proteins, polysaccharides, polyphenols, flavonoids and triterpenoids. [0003] Flavonoids (flavonoids) are a class of compounds that exist in nature and have...

AI Technical Summary

Problems solved by technology

[0005] The traditional separation of guava leaf extract mainly adopts solvent method, macroporous resin adsorption method, microwave extraction method, ultrasonic extraction method, supercritical extraction method, but these methods are complicated in process, poor in purification effect, and do not meet the requirements of energy saving and environmental protection. High cost and low benefit

However, capillary electrophoresis and enzymatic methods are generally used to purify trace or trace substances, which have high requirements on purification conditions and are not suitable for industrial purification.

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