Yellow fever antigen near infrared fluorescence detection kit and application thereof

A fluorescence detection, near-infrared technology, applied in measurement devices, immunoassays, resistance to vector-borne diseases, etc., can solve the problems of panic and low immunity, and achieve the effects of good specificity, good specificity and high sensitivity

Inactive Publication Date: 2018-06-29
JIANGSU WISE SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there is no large-scale epidemic of yellow fever in my country at present, the geographical conditions in some areas in southern China are similar to areas with high incidence of yellow fever, and there are host animals sensitive to yellow fever virus and vectors such as Aedes aegypti. There is a possibility of disease. At the same time, because there has never been a large-scale epidemic of yellow fever in my country before, Chinese residents have low immunity to it. Once an outbreak occurs, it will definitely cause panic.

Method used

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  • Yellow fever antigen near infrared fluorescence detection kit and application thereof

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Effect test

Embodiment 1

[0056] 1. Sputum collection and storage

[0057] Under the guidance of medical staff, the patient coughs up the sputum in the deep part of the respiratory tract forcefully, spits it into a sterile jar, and immediately closes the lid tightly and saves it for later use. The samples to be processed should be stored in a refrigerator at 4°C.

[0058] 2. Urine collection and storage

[0059] Under the guidance of medical staff, the patient washes and collects the first urine in the morning, and the collected urine is stored in a clean and sterile container for future use. Urine samples can be stored at 2-8°C for 72 hours. If they need to be stored for a longer period of time, the samples must be stored at -20°C. Repeated freezing and thawing of samples should be avoided, otherwise erroneous experimental results will be produced. Samples should not be stored in an automatic defrost refrigerator.

[0060] 3. Antigen preparation:

[0061] The yellow fever strain purchased from ATC...

Embodiment 2

[0070] Comparative experiment of yellow fever antigen near-infrared fluorescence detection method and sputum bacterial culture:

[0071] Bacterial culture of sputum: the sputum was pretreated (the method of combining 1ug / ml trypsin solution with glass fragment oscillation was used to pretreat the sputum before inoculation and culture), and the pretreated samples were immediately inoculated in GVPC selective On the culture medium, carry out Gram staining on the suspected yellow fever colonies, and inoculate the suspected Gram-staining negative colonies on BCYE-a and BCYE-Cys agar plates, and cultivate them in a 37°C incubator for 2 days. Any colony that grows on BCYE-a medium but does not grow on BCYE-Cys medium can be considered as yellow fever.

[0072] Kit detection:

[0073] The sample to be tested is sputum diluent, which is diluted with 0.01M PBS buffer solution with pH7.2, and the dilution ratio is 1:1. The sample to be tested and the kit are equilibrated to room tempe...

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Abstract

The invention relates to the technical field of biologics and in particular relates to a yellow fever antigen near infrared fluorescence detection kit and application thereof. The invention provides the yellow fever antigen near infrared fluorescence detection kit which comprises a reagent strip, wherein the reagent strip is positioned on a back plate; the reagent strip comprises a sample pad, a glass cellulose membrane marked by using an immunofluorescence probe, a nitrocellulose membrane coated by an LP antibody, and water absorption paper in sequence from a sample feeding end. By adopting the detection kit provided by the invention, urea or sputum of a patient can be adopted as a detection sample, and with the reference to the detection kit and a near-infrared fluorescence detection technique, specific and dissoluble LP antigen in a patient specimen can be rapidly and accurately detected.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a near-infrared fluorescence detection kit for yellow fever antigen and its application. Background technique [0002] Yellow fever is an acute viral infectious disease characterized by fever, jaundice, and hemorrhage. In modern times, due to environmental damage, increased international travel, and reduced vaccine coverage in yellow fever-affected countries, yellow fever has re-emerged in parts of Africa and South America since 1979. It is estimated that there are actually 200,000 patients worldwide each year , the death toll reached 30 000, while only a small number were diagnosed. Although there is no large-scale epidemic of yellow fever in my country at present, the geographical conditions in some areas in southern China are similar to areas with high incidence of yellow fever, and there are host animals sensitive to yellow fever virus and vectors such as Aedes aegypti. There is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/543G01N33/533
CPCG01N33/533G01N33/54313G01N33/56983G01N33/577G01N2469/10Y02A50/30
Inventor 洪霞刘静立雯馨
Owner JIANGSU WISE SCI & TECH DEV
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