A preparation method of molecularly imprinted polymer microspheres for the detection of 2,4,6-trinitrophenol
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A technology of trinitrophenol and molecular imprinting, applied in the field of detection 2, to achieve the effect of large specific surface area, small surface resistance and many recognition sites
Active Publication Date: 2021-05-25
霍尔姆斯(北京)生物科技有限公司
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[0010] Although the above methods have their own characteristics, there is no report in the literature that the target molecule itself has fluorescent properties, and the target molecule with yellow fluorescence enters the recognition site through its own optical signal sensitive output.
At the same time, there is no report in the literature that the polynitrophenol derivatives with bright yellow fluorescence produced by the reaction of 2,4,6-trinitrophenol and N,N'-diisopropylcarbodiimide are imprinted into polymer microparticles. Spheres, imprinted derivatives that enter the recognition site, enable reporting of recognition and detection by switching on and off of autofluorescence
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[0052] A method for preparing molecularly imprinted polymer microspheres for the detection of 2,4,6-trinitrophenol, characterized in that: 2,4,6-trinitrophenol and N,N'-diisopropyl The polynitrophenol derivatives generated by the carbodiimide reaction have bright yellow fluorescence, and their fluorescence has opening and closing characteristics under acid-base conditions. Molecules, the inner part of the imprinting layer has a cavity structure complementary to the structure, size and functional groups of the imprinted molecules, and the polymer microspheres that elute the imprinted molecules have specific recognition sites for the target analyte molecules, realizing the target analyte Molecular selective recognition and detection, the above preparation process includes the following four steps:
[0053] The first step is the synthesis of 2,4,6-trinitrophenol derivatives: Weigh 0.4-0.6 g of the treated 2,4,6-trinitrophenol into a 100 mL three-necked flask, and then add 7-8 Af...
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[0059] 2,4,6-Trinitrophenol and N,N'-diisopropylcarbodiimide are produced by nucleophilic addition reaction of polynitrophenol derivatives, which can be separated and purified to obtain 2,4,6- Molecularly imprinted polymer microspheres were prepared by copolymerization of trinitrophenol derivative PAD1 with functional monomers, cross-linking agents and initiators, and polymer microspheres with selective recognition of PAD1 and fluorescent signal output were obtained after the template molecule PAD1 was eluted .
[0060] The first step is the synthesis of 2,4,6-trinitrophenol derivatives: weigh 0.5 g of the treated 2,4,6-trinitrophenol into a 100 mL three-necked flask, and then add 7.5 mL of dichloromethane Ultrasonic dispersion, then add 0.3 g of N,N'-diisopropylcarbodiimide, the solution immediately turns reddish brown, stir for 2 h under nitrogen atmosphere, the solution becomes viscous due to the volatilization of solvent dichloromethane and dried in a vacuum oven at 40 °C...
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Abstract
A method for preparing molecularly imprinted polymer microspheres for 2,4,6-trinitrophenol detection, characterized in that: 2,4,6-trinitrophenol and N,N'-diisopropyl The imprinted molecules of polynitrophenol derivatives produced by the carbodiimide reaction have bright yellow fluorescence, and their fluorescence has opening and closing characteristics under acid-base conditions, and the elution in the molecularly imprinted polymer microspheres is located in the imprinted layer The imprinted molecules of the imprinted layer have a cavity structure complementary to the structure, size and functional groups of the imprinted molecules, and the polymer microspheres that elute the imprinted molecules have specific recognition sites for target analyte molecules, and the target molecules enter The specific recognition site realizes the selective recognition of the target analyte molecule, and realizes the sensitive detection through the opening and closing of the fluorescence of the target molecule. The specific surface area of the molecularly imprinted microsphere is large, and the spatial structure of the recognition site is stable and effective. Multiple spots, highly selective recognition and highly sensitive detection of target molecules.
Description
technical field [0001] The invention relates to the field of material science, in particular to a preparation method for detecting 2,4,6-trinitrophenol molecular imprint. Background technique [0002] International relations in today's world are complex and changeable, and terrorist and violent incidents such as bombings occur frequently, which seriously endanger the lives and property safety of people all over the world and social stability, and pose a threat to the security and stability of countries and regions. Therefore, the detection and analysis of explosives has attracted great attention from research institutions in various countries, trying to predict the existence of explosives and reduce the harm of explosives to humans through analysis and detection technology. 2,4,6-trinitrophenol (2,4,6-trinitrophenol, TNP) belongs to phenolic substance, also known as picric acid (picric acid, PA). Its raw materials are easy to obtain, its preparation is simple, and its explo...
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