Method for producing edible and medicinal fungus peptides by performing enzymolysis thrice and fermentation once

A production method and technology for medicinal bacteria, which are applied in the production field of edible medicinal microbial peptides with three enzymes and one fermentation, can solve the problems of low content of small peptides, limited protein absorption, incomplete enzymatic hydrolysis, etc., and achieve protein degradation. Thorough, simplified operation, increased productivity

Inactive Publication Date: 2018-07-06
安徽精准医疗产业研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the human body’s absorption of protein is limited after edible fungi are directly eaten. In order to improve its absorption effect, protein powder can be decomposed into small peptides to improve the nutritional value of edible fungi. However, most of the existing decomposition methods use protease enzymatic hydrolysis. However, the content of the obtained small peptides is often low due to the incomplete degree of enzymatic hydrolysis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The production method of three-enzyme-one-ferment Ganoderma lucidum mycin includes the following steps:

[0027] S1: Crush the Ganoderma lucidum, and add distilled water to prepare the raw material liquid according to the material-to-liquid ratio of 1g: 15mL, and use HAc to adjust the pH of the raw material liquid to 4.5, then add cellulase to enzymatically hydrolyze at 65℃ for 2h; Enzyme activity is 1200U / g;

[0028] S2: Repeatedly freeze and thaw the enzymatic hydrolysate at -25°C for 2 times. After the enzymatic hydrolysate is naturally warmed to room temperature, ultrasound for 30 minutes and use NH 4 Ac adjusts the pH to 7.0; then continue to add papain and neutral protease to the enzymolysis solution at 55°C for 5h; the added amount of papain is 650U / g, and the added amount of neutral protease is 800U / g;

[0029] S3: After the enzymolysis is completed, put the enzymatic hydrolysate into the fermenter, and the number of live bacteria is 4×10 according to the 0.3% inoculum...

Embodiment 2

[0032] The production method of three-enzyme-one-fermented mushroom mycin includes the following steps:

[0033] S1: Crush the mushrooms, add distilled water to prepare the raw material liquid according to the material-to-liquid ratio of 1g:20mL, and use HAc to adjust the pH of the raw material liquid to 5.5, then add cellulase to enzymatically hydrolyze at 70℃ for 1.5h; The enzyme activity in the solution is 1500U / g;

[0034] S2: Repeatedly freeze and thaw the enzymatic hydrolysate at -15°C twice, after the enzymatic hydrolysate is naturally warmed to room temperature, ultrasound for 20 minutes and use NH 4 Ac adjust the pH to 7.2; then continue to add papain and neutral protease to the enzymolysis solution at 65°C for 4 hours; the addition amount of papain is 500 U / g, and the addition amount of neutral protease is 1000 U / g;

[0035] S3: After the enzymolysis is completed, put the enzymatic hydrolysate into the fermenter, and insert the number of viable bacteria at 0.5% of the inocu...

Embodiment 3

[0039] Application of edible fungi peptide

[0040] The Ganoderma lucidum mycin prepared in Example 1 and other auxiliary materials were used to prepare mycin drink. The specific formula is: 10 parts of Ganoderma lucidum mycotin, 2 parts of honey, 0.5 parts of taurine, 1.5 parts of citric acid, 1.0 part of potassium sorbate, 2.0 parts of pectin, and 80 parts of water. The mycotin drink can significantly enhance the body's immunity and regulate body functions.

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PUM

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Abstract

The invention discloses a method for producing edible and medicinal fungus peptides by performing enzymolysis thrice and fermentation once. The method comprises the following steps: pulverizing edibleand medicinal fungi, and adding cellulase for performing enzymolysis; adding papain and neutral protease into the enzymolysis liquid for performing enzymolysis; after the enzymolysis is completed, inoculating bacillus subtilis for performing fermentation; and after the fermentation is completed, centrifuging the fermentation liquid, then taking supernatant liquid, performing filtration with an ultra-filtration membrane, and performing purification, sterilization, concentration and drying to obtain the edible and medicinal fungus peptides. The method disclosed by the invention has the advantages that fungi and enzymes are mutually matched to produce small peptides, respective advantages of enzymolysis and microorganisms are fully utilized, and synergistic effects of the microorganisms andproteases are exerted to ensure that the protein of the edible and medicinal fungi can be degraded more thoroughly and the degree of small peptides is higher; and during subsequent purification, an ultra-filtration membrane is utilized for performing filtration and ion exchange resin is utilized for performing purification so as to obtain high-content edible and medicinal fungus peptides.

Description

Technical field [0001] The invention relates to a production method of three-enzyme-one-fermented edible and medicinal mycotin. Background technique [0002] Edible fungus is a large-scale fungus that is both edible and medicinal. It is known as a "mountain treasure". The types of amino acids, vitamins and minerals contained in edible fungi are extremely rich, especially those that are necessary for the human body. A variety of amino acids. The protein content of edible fungi is close to that of pigs, cattle, mutton and poultry eggs, while the fat content is significantly lower than that of pigs, cattle, and muttons. In particular, edible fungi such as ginseng and cordyceps have immunoregulatory effects and delay cells. The aging and anti-tumor effects have very high application value and development prospects. [0003] However, after the edible fungus is eaten directly, the human body has limited absorption of protein. In order to improve its absorption effect, the protein powder...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12P21/00C07K1/36C07K1/34C07K1/18C12R1/125
CPCC12P21/00C12P21/06
Inventor 不公告发明人
Owner 安徽精准医疗产业研究院有限公司
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