Method for producing edible and medicinal fungus peptides by performing enzymolysis thrice and fermentation once
A production method and technology for medicinal bacteria, which are applied in the production field of edible medicinal microbial peptides with three enzymes and one fermentation, can solve the problems of low content of small peptides, limited protein absorption, incomplete enzymatic hydrolysis, etc., and achieve protein degradation. Thorough, simplified operation, increased productivity
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Embodiment 1
[0026] The production method of three-enzyme-one-ferment Ganoderma lucidum mycin includes the following steps:
[0027] S1: Crush the Ganoderma lucidum, and add distilled water to prepare the raw material liquid according to the material-to-liquid ratio of 1g: 15mL, and use HAc to adjust the pH of the raw material liquid to 4.5, then add cellulase to enzymatically hydrolyze at 65℃ for 2h; Enzyme activity is 1200U / g;
[0028] S2: Repeatedly freeze and thaw the enzymatic hydrolysate at -25°C for 2 times. After the enzymatic hydrolysate is naturally warmed to room temperature, ultrasound for 30 minutes and use NH 4 Ac adjusts the pH to 7.0; then continue to add papain and neutral protease to the enzymolysis solution at 55°C for 5h; the added amount of papain is 650U / g, and the added amount of neutral protease is 800U / g;
[0029] S3: After the enzymolysis is completed, put the enzymatic hydrolysate into the fermenter, and the number of live bacteria is 4×10 according to the 0.3% inoculum...
Embodiment 2
[0032] The production method of three-enzyme-one-fermented mushroom mycin includes the following steps:
[0033] S1: Crush the mushrooms, add distilled water to prepare the raw material liquid according to the material-to-liquid ratio of 1g:20mL, and use HAc to adjust the pH of the raw material liquid to 5.5, then add cellulase to enzymatically hydrolyze at 70℃ for 1.5h; The enzyme activity in the solution is 1500U / g;
[0034] S2: Repeatedly freeze and thaw the enzymatic hydrolysate at -15°C twice, after the enzymatic hydrolysate is naturally warmed to room temperature, ultrasound for 20 minutes and use NH 4 Ac adjust the pH to 7.2; then continue to add papain and neutral protease to the enzymolysis solution at 65°C for 4 hours; the addition amount of papain is 500 U / g, and the addition amount of neutral protease is 1000 U / g;
[0035] S3: After the enzymolysis is completed, put the enzymatic hydrolysate into the fermenter, and insert the number of viable bacteria at 0.5% of the inocu...
Embodiment 3
[0039] Application of edible fungi peptide
[0040] The Ganoderma lucidum mycin prepared in Example 1 and other auxiliary materials were used to prepare mycin drink. The specific formula is: 10 parts of Ganoderma lucidum mycotin, 2 parts of honey, 0.5 parts of taurine, 1.5 parts of citric acid, 1.0 part of potassium sorbate, 2.0 parts of pectin, and 80 parts of water. The mycotin drink can significantly enhance the body's immunity and regulate body functions.
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