A kind of method utilizing crystal violet to measure melamine
A technology based on melamine and crystal violet, which is applied in measuring devices, material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of high cost and time-consuming analysis, and achieve high sensitivity, simple and fast operation
Active Publication Date: 2020-07-31
GUILIN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology
Chromatography is mostly used for the determination of melamine. Although the detection accuracy is high, the analysis cost is high and time-consuming; in addition, there are chemiluminescence, electrochemistry, spectrophotometry, fluorescence and resonance scattering spectroscopy, etc.
Crystal Violet (Crystal Violet, CV) is a triphenylmethane series basic dye, commonly used in the determination of metal ions, but the use of crystal violet and melamine reaction, using resonance scattering spectroscopy to detect the content of melamine has not been reported in the literature
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[0013] (1) Add 0.1, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 mL of melamine solution with a concentration of 1000 μg / L to eight 10 mL colorimetric tubes, and then add 1.0 mL to each colorimetric tube Britton-Robinson (Britton-Robinson, B-R) buffer solution at pH 2.4, 1.0 mL concentration is 4.0×10 -5 mol / L crystal violet solution, use the solution without melamine as the reagent blank, then use ultrapure water to dilute the volume of 8 colorimetric tubes to the scale, react in a water bath at 25°C for 10 minutes, and then put it on the fluorescence photometer , use a 1cm cuvette, set λem=λex=394nm, slit 5nm, voltage 600V, respectively measure the scattered light intensity I of the solution containing melamine and the scattered light intensity I of the reagent blank 0 , calculate the scattered light intensity difference ΔI=I-I 0 , the scattered light intensity difference ΔI is linearly related to the melamine mass concentration ρ in the range of 10-600 μg / L, the linear regression equ...
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The invention discloses a method of determining melamine with crystal violet. The method comprises the steps of sequentially adding 0.1mL, 0.5mL, 1.0mL, 2.0mL, 3.0mL, 4.0mL, 5.0mL and 6.0mL 1000mcg / Lmelamine solution into eight 10mL colorimetric tubes, then adding a Britton-Robinson buffer solution and a crystal violet solution separately, taking a solution without melamine as a reagent blank, increasing a volume to a scale, determining scattering intensity I of the solutions containing melamine and scattering intensity I0 of the reagent blank on a fluorophotometer with a 1cm cuvette under the conditions of lambda em equal to lambda ex equal to 394nm, a 5nm slit and 600V voltage after a reaction, and calculating a scattering intensity difference delta I by subtracting I0 from I. A trichloroacetic acid and lead acetate solution is added into pure milk; then, ultrasound treatment and centrifugation are performed; a scattering intensity value is determined by the method; and a melamine content in the milk is calculated. The method is high in sensitivity and easy, simple and quick to operate.
Description
technical field [0001] The invention relates to a method for measuring melamine, in particular to a method for measuring melamine by utilizing crystal violet and adopting a resonance scattering technique. Background technique [0002] Melamine (Melamine, MEL) is an important nitrogen-heterocyclic organic chemical raw material, mainly used in the production of melamine-formaldehyde resin, and is also widely used in the production of textile auxiliaries, leather emollients, glazing agents, water repellents, adhesives, etc. middle. Adding melamine to fresh milk and dairy products can significantly increase the nitrogen content, which can be used to pretend to be high-protein foods, thereby greatly reducing costs. Since the outbreak of tainted milk powder incident in 2008, the rapid detection of melamine in food has become an urgent problem to be solved. Chromatography is mostly used for the determination of melamine. Although the detection accuracy is high, the analysis cost ...
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IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/643G01N2021/6417
Inventor 唐宁莉范晶晶马小龙王文平黄文琪张容珲
Owner GUILIN UNIVERSITY OF TECHNOLOGY