Application of SL-LINC2 gene endowing plant with yellow leaf curl virus disease resistance

A technology of SL-LINC2, yellow leaf curl virus, applied in the application, plant products, genetic engineering and other directions, can solve the problems that have not been reported, and achieve the effect of improving resistance

Inactive Publication Date: 2018-07-10
JIANGSU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on lncRNA related to tomato resistance to yellow leaf curl virus disease

Method used

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  • Application of SL-LINC2 gene endowing plant with yellow leaf curl virus disease resistance
  • Application of SL-LINC2 gene endowing plant with yellow leaf curl virus disease resistance
  • Application of SL-LINC2 gene endowing plant with yellow leaf curl virus disease resistance

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Experimental program
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Embodiment Construction

[0051] The methods used in the following embodiments are conventional methods unless otherwise specified, and the primer sequences used are all synthesized by Nanjing GenScript Biotechnology Co., Ltd., and the stated percentages are all mass percentages. The gene in this experiment was derived from tomato yellow leaf curl virus disease-resistant material (CLN2777A).

[0052] 1) tomato SL-LINC2 gene cloning

[0053] The TYLCV-resistant tomato variety CLN2777A (Chen et al., 2013) was selected. After the tomato grows to the 3-4 leaf stage, the tomato leaves were quickly frozen in liquid nitrogen, ground in a mortar, and the RNA extraction kit (DP419) of Beijing Tiangen Company was used. Total RNA was extracted, and the quality of total RNA was identified by electrophoresis; according to tomato RNA-Seq data (Chen TZ. et al, 2013), 529 long-chain non-coding RNA genes that may be related to resistance were identified. Through qRT-PCR verification, it was found that multiple ESTs w...

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Abstract

Belonging to the field of plant genetic engineering, the invention relates to a tomato non-coding RNA gene SL-LINC2 and application thereof. The cDNA sequence SEQ ID NO.1 of the gene is reported for the first time in tomatoes. Expression analysis indicates that the gene has up-regulated expression after TYLCV (tomato yellow leaf curl virus) induction, PCR and RT-PCR verification are carried out onan obtained transgenic tomato plant, then TYLCV is inoculated for disease resistance evaluation, and the result shows that compared with the control group, the transgenic plant has significantly improved resistance to TYLCV.

Description

1. Technical field [0001] The present invention relates to a method for imparting resistance to yellow leaf curl virus disease in plants SL-LINC2 Gene and its application in plant resistance to yellow leaf curl virus disease. 2. Background technology [0002] Tomato yellow leaf curl virus disease is one of the important diseases that cause worldwide tomato quality and yield reduction. Virus (Tomato Yellow Leaf Curl Virus, TYLCV). At present, the most effective way to solve this problem in production is to plant disease-resistant varieties. The resistance genes of tomato yellow leaf curl virus that have been reported so far are: Ty-1 , Ty-2 , Ty-3 , Ty- 3a , Ty-4 with Ty-5 Wait. These genes were all derived from wild tomato varieties. In view of the lack of TYLCV resistance genes in tomato cultivar resources, cloning of TYLCV-resistant genes that can be applied to production has important theoretical significance and practical value. [0003] Long-chain non-c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00
CPCC12N15/1131C12N15/8218C12N15/8283C12N2310/10
Inventor 杨郁文黄俊宇张保龙王金彦张雨濛周宁
Owner JIANGSU ACAD OF AGRI SCI
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