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Liquid chromatography method for detecting content of olanzapine drug in blood

A liquid chromatographic analysis and olanzapine technology, applied in the field of blood drug concentration monitoring of psychotropic drugs, can solve the problems of high personnel and site requirements, large amount of reagents, high sensitivity, and achieve environmental protection of experimental costs and reduction of experimental costs. , reducing the effect of toxic and side effects

Inactive Publication Date: 2018-07-10
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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Problems solved by technology

LC-MS / MS method has high sensitivity, but poor stability, high cost, and high requirements for personnel and site
2D-LC / UV has good separation effect, high sensitivity, high robustness and automation capability, but the current detection method requires a large amount of blood, and the amount of reagents consumed in the pretreatment process is also large

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  • Liquid chromatography method for detecting content of olanzapine drug in blood
  • Liquid chromatography method for detecting content of olanzapine drug in blood
  • Liquid chromatography method for detecting content of olanzapine drug in blood

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Embodiment Construction

[0034] The liquid chromatographic analysis method of detection blood olanzapine drug content of the present invention, it comprises the following steps:

[0035] (1) Calibration of standard solution

[0036] First, 10 μL and 190 μL of standard working solutions of seven different concentrations were placed in seven centrifuge tubes of 1.5 ml and mixed to make seven standard solutions. Blank serum or plasma refers to serum or plasma that does not contain olanzapine. Plasma, the above standard solutions were vortexed at 1000rpm for 1min, then 1000μL of extractant consisting of 9:1 methyl tert-butyl ether and ethyl acetate was added, and vortexed at 1000rpm After 5 minutes, add 200 μL of 10 mol / L NaOH solution, vortex and mix at 2000 rpm for 2 minutes, then centrifuge at 12000 rpm for 10 minutes to obtain clear liquid, take 720 ul of the above clear liquid and place in another 1.5 ml centrifuge tube at room temperature with N 2 Dry slowly; add 100 μL of a complex solution compo...

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Abstract

A liquid chromatography method for detecting the content of an olanzapine drug in blood comprises the following steps: calibrating standard solutions by using a liquid chromatograph and an ultravioletdetector, performing fitting to obtain a standard curve equation y = a*x + b, taking a blood sample to be detected, processing the blood to be detected, detecting the blood to be detected by using the liquid chromatograph and the ultraviolet detector to obtain the y value of the blood to be detected, substituting the y value of the blood to be detected into the standard curve equation, and calculating the concentration of the olanzapine drug in the blood to be detected. The method combines an external standard technology with high performance liquid chromatography, and is suitable for high-throughput sample detection; and a small particle size column and a low flow rate are adopted to reduce the consumption of an organic solvent, so the experimental cost is reduced, environmental protection is achieved, monitoring the plasma concentration of olanzapine in a patient during clinical treatment is facilitated, and an experimental basis is provided for the personalized administration of the antipsychotic drug olanzapine and the reduction of toxic and side effects.

Description

technical field [0001] The invention relates to the technical field of blood drug concentration monitoring of psychotropic drugs, in particular to a method for detecting the drug concentration of olanzapine in blood. Background technique [0002] Olanzapine (OLZ), molecular formula C 17 h 20 N 4 S, the specific structure is as follows figure 1 shown. Olanzapine is a yellow crystalline powder with a melting point of 191-196°C. Schizophrenia is a common mental disorder, and the lifetime incidence rate of adults in my country is about 1%-2%. Olanzapine is a commonly used traditional atypical antipsychotic drug, which is rapidly absorbed in the body and has strong sedative and hypnotic effects. It is used for the treatment of schizophrenia, psychotic disorders and affective disorders. The recommended daily dose of olanzapine is 5-20 mg, and the effective serum or plasma threshold concentration for the treatment of schizophrenia is only 9 μg / L as reported in the literature....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 魏斌贾永娟倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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