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Inoculation mud inoculation method for rapidly enriching nitrous acid type denitrification anaerobic methane oxidation microorganisms

An anaerobic oxidation and microbial technology is applied in the field of seed sludge inoculation for rapid enrichment of denitrifying anaerobic methane oxidizing microorganisms, which can solve the problems of long enrichment time, slow growth and low activity, avoid secondary pollution, shorten the The effect of enrichment time, easy operation and maintenance

Inactive Publication Date: 2018-07-13
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since DAMO microorganisms are autotrophic microorganisms, they grow slowly and their doubling time is several weeks or even months, so the problems of long enrichment time and low activity have always been the main factors that limit the research of DAMO microorganisms and the development and application of related technologies.
[0006] At present, there are few studies on the inoculum slime, but the abundance of DAMO microorganisms in the inoculum slime directly affects the enrichment rate and the stability of the system during the enrichment process.
The best enrichment environment is achieved through the improved reactor, the configuration of the improved reactor is generally more complicated, and the operation and maintenance are difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Example 1 Using a variety of mixed activated sludge as inoculum, enriching nitrite-type anaerobic methane oxidizing bacteria (n-DAMO) in the airlift sequencing batch bioreactor system, using airlift sequencing batch bioreactor The reactor is used as a reactor to start the rapid enrichment of n-DAMO, and inoculate the activated sludge after sewage mixing treatment. The temperature of the sequencing batch bioreactor is maintained between 30 °C and 38 °C, and the pH is controlled at 7-8. The reactor adopts the method of artificial water inflow, and the ratio of the water inflow volume to the total volume is controlled at 40 %-50 % between. Start the reactor for rapid enrichment of n-DAMO in the following 4 stages.

[0023] The first stage: Mix 50 mL of seawater sediment, 50 mL of reservoir sediment, 50 mL of freshwater lake sediment, 100 mL of anaerobic digester sludge, 50 mL of paddy field sediment and 100 mL of industrial methane fermentation sediment, Flush with norma...

example 2

[0027] Example 2 The nitrate-type anaerobic methane oxidizing archaea (DAMO) were enriched in an air-lift sequencing batch bioreactor system using various mixed activated sludge as inoculum. According to the experimental procedure of Example 1, the nitrogen source in the medium was replaced with NaNO 3 , NO 3 - -N concentration is controlled between 30-100 mg / L, and the operation of the reactor is the same as in Example 1.

example 3

[0028] Example 3 Synchronous enrichment of nitrite-type anaerobic methane-oxidizing bacteria (n-DAMO) and nitrate-type anaerobic methane oxidation bacteria in an airlift sequencing batch bioreactor system using various mixed activated sludge as inoculum sludge Archaea (DAMO). According to the experimental procedure of Example 1, the nitrogen source in the medium was replaced with NaNO 2 and NaNO 3 , the nitrogen load concentration is controlled between 30-100 mg / L, and the operation of the reactor is the same as that of Example 1.

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Abstract

The invention relates to an inoculation mud inoculation method for rapidly enriching nitrous acid type denitrification anaerobic methane oxidation microorganisms. The inoculation mud inoculation method comprises: (1) mixing anaerobic sludge having different conditions according to a certain ratio, and pre-treating to obtain the inoculation sludge of an enrichment system; and (2) inoculating the mixed sludge obtained in the step (1) into an airlift type continuous batch reactor, adding a nitrogen source, methane, trace elements and other liquid culture mediums during culture, and enriching denitrification anaerobic methane oxidation microorganisms within a short time. According to the present invention, the inoculation mud obtained by mixing according to a certain ratio is used to enrich, such that the microbial ecological stability during the enriching can be improved and the denitrification anaerobic methane oxidation microorganism enriching time can be substantially shortened compared to the single inoculation mud.

Description

technical field [0001] The invention relates to the technical field of autotrophic anaerobic microorganism enrichment, in particular to a seed sludge inoculation method for rapidly enriching denitrifying anaerobic methane oxidizing microorganisms. Background technique [0002] Methane (CH 4 ) is not only an energy substance, but also a strong greenhouse gas, CH per unit mass 4 [0003] The heat absorption is about CO 2 25 times. The microbial denitrification process refers to the process in which nitrate nitrogen and nitrite nitrogen are reduced to gaseous nitrogen under the action of denitrifying bacteria. It is one of the main processes for biological denitrification in the field of sewage treatment. In the classic denitrification process, denitrifying bacteria use nitrogen oxides as electron acceptors and organic matter, namely carbon sources, as electron donors. Denitrifying anaerobic methane oxidation (DAMO) is a newly discovered nitrate biological reduction proces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36
CPCC12N1/36
Inventor 张召基王劲松陈少华王晓君叶欣
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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