A kind of process of fermenting and preparing citric acid
A citric acid and process technology, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of high cost of medium, low yield of citric acid, etc., to promote water penetration and shorten fermentation culture time , the effect of increasing production
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Embodiment 1
[0025] A process for fermenting and preparing citric acid, comprising the steps of:
[0026] Preparation of fermentation medium: crush corn stalks, then mix them with corn husks at a mass ratio of 2:1, crush them to a particle size of 50 mesh, then add them to 5 times the weight of water, heat up to 80°C while stirring, and keep warm for 30 minutes. Then add potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate heptahydrate 0.2g / L, ferrous sulfate heptahydrate 0.01g / L, manganese sulfate monohydrate 0.01g / L, extinguish with damp heat at 121℃ Bacteria 30min, cooling, obtains Trichoderma nutrient solution; With Trichoderma reesei ATCC 13631 according to 10% inoculum amount (inoculation density is 5 * 10 6 cfu / mL) into the Trichoderma culture medium, cultured for 72 hours, the stirring speed was controlled at 100rpm during the culture, the culture temperature was 30-32°C, and the pH of the culture fermentation process was controlled to be 4-5 ...
Embodiment 2
[0030] A process for fermenting and preparing citric acid, comprising the steps of:
[0031] Preparation of fermentation medium: crush corn stalks, then mix them with corn husks at a mass ratio of 2:1, crush them to a particle size of 100 mesh, then add them to 6 times the weight of water, heat up to 80°C while stirring, and keep warm for 30 minutes. Then add potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate heptahydrate 0.2g / L, ferrous sulfate heptahydrate 0.01g / L, manganese sulfate monohydrate 0.01g / L, extinguish with damp heat at 121℃ Bacteria 30min, cooling, obtains Trichoderma nutrient solution; Trichoderma reesei is according to 10% inoculum size (inoculation density is 5 * 10 6 cfu / mL) into the Trichoderma culture medium, cultured for 96 hours, the stirring speed was controlled at 100rpm during the culture, the culture temperature was 30-32°C, and the pH of the culture fermentation process was controlled to be 4-5 by feeding ammon...
Embodiment 3
[0037] Taking Example 1 as an example, the mixed fermentation acid production of Trichoderma reesei and Aspergillus niger was compared, and the specific comparison results are shown in Table 1:
[0038] Table 1
[0039] Fermentation method Acid production g / L, Residual sugar % Fermentation period h mixed fermentation 129 0.21 60 Example 1 151 0.18 60
[0040] As shown in Table 1 above, when Trichoderma reesei and Aspergillus niger were co-fermented, although the two strains could co-exist well, the production of citric acid decreased by 16%, which may be due to the fact that Trichoderma reesei used more nitrogen source, making the nutrient substance of Aspergillus niger relatively deficient, it may also be that Trichoderma reesei produced extracellular secretions that inhibited the citric acid pathway of Aspergillus niger, thereby reducing the output of citric acid; Trichoderma needs to be removed after the culture is completed.
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