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A kind of process of fermenting and preparing citric acid

A citric acid and process technology, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of high cost of medium, low yield of citric acid, etc., to promote water penetration and shorten fermentation culture time , the effect of increasing production

Active Publication Date: 2020-11-10
齐齐哈尔龙江阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention is to solve the defects of high cost of fermentation medium and low yield of citric acid in the prior art, and provide a process for preparing citric acid by fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A process for fermenting and preparing citric acid, comprising the steps of:

[0026] Preparation of fermentation medium: crush corn stalks, then mix them with corn husks at a mass ratio of 2:1, crush them to a particle size of 50 mesh, then add them to 5 times the weight of water, heat up to 80°C while stirring, and keep warm for 30 minutes. Then add potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate heptahydrate 0.2g / L, ferrous sulfate heptahydrate 0.01g / L, manganese sulfate monohydrate 0.01g / L, extinguish with damp heat at 121℃ Bacteria 30min, cooling, obtains Trichoderma nutrient solution; With Trichoderma reesei ATCC 13631 according to 10% inoculum amount (inoculation density is 5 * 10 6 cfu / mL) into the Trichoderma culture medium, cultured for 72 hours, the stirring speed was controlled at 100rpm during the culture, the culture temperature was 30-32°C, and the pH of the culture fermentation process was controlled to be 4-5 ...

Embodiment 2

[0030] A process for fermenting and preparing citric acid, comprising the steps of:

[0031] Preparation of fermentation medium: crush corn stalks, then mix them with corn husks at a mass ratio of 2:1, crush them to a particle size of 100 mesh, then add them to 6 times the weight of water, heat up to 80°C while stirring, and keep warm for 30 minutes. Then add potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate heptahydrate 0.2g / L, ferrous sulfate heptahydrate 0.01g / L, manganese sulfate monohydrate 0.01g / L, extinguish with damp heat at 121℃ Bacteria 30min, cooling, obtains Trichoderma nutrient solution; Trichoderma reesei is according to 10% inoculum size (inoculation density is 5 * 10 6 cfu / mL) into the Trichoderma culture medium, cultured for 96 hours, the stirring speed was controlled at 100rpm during the culture, the culture temperature was 30-32°C, and the pH of the culture fermentation process was controlled to be 4-5 by feeding ammon...

Embodiment 3

[0037] Taking Example 1 as an example, the mixed fermentation acid production of Trichoderma reesei and Aspergillus niger was compared, and the specific comparison results are shown in Table 1:

[0038] Table 1

[0039] Fermentation method Acid production g / L, Residual sugar % Fermentation period h mixed fermentation 129 0.21 60 Example 1 151 0.18 60

[0040] As shown in Table 1 above, when Trichoderma reesei and Aspergillus niger were co-fermented, although the two strains could co-exist well, the production of citric acid decreased by 16%, which may be due to the fact that Trichoderma reesei used more nitrogen source, making the nutrient substance of Aspergillus niger relatively deficient, it may also be that Trichoderma reesei produced extracellular secretions that inhibited the citric acid pathway of Aspergillus niger, thereby reducing the output of citric acid; Trichoderma needs to be removed after the culture is completed.

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PUM

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Abstract

The invention belongs to the technical field of microbial fermentation, and discloses a technology for preparing citric acid through fermentation. The technology comprises the following steps: step (1) preparing a fermentation culture medium; step (2) activating an aspergillus niger seed solution; step (3) producing acid through fermentation. The technology for preparing the citric acid through fermentation, disclosed by the invention, is low in cost and high in yield of the citric acid.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a process for preparing citric acid by fermentation. Background technique [0002] Citric acid, also known as citric acid, scientific name 2-hydroxy-l, 2, 3-propanetricarboxylic acid (2-hydroxy-1, 2, 3-propanetricanboxylic acid), is a white crystal powder, tasteless, often contains one molecule Water of crystallization or no water of crystallization, easily soluble in water. It is one of the main intermediates of the triple acid cycle in organisms, so it has important metabolic significance in almost all organisms. Citric acid is widely used in food, medicine, cosmetics, chemical industry and other fields. Since citric acid has a refreshing sour taste, it is widely used as an acidic flavoring agent for various beverages and snacks, or as an antioxidant for edible oils. In addition, citric acid has the effect of preventing or eliminating skin pigmenta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/48C12R1/685
CPCC12P7/48
Inventor 王铮董力青唐永强王峰李航
Owner 齐齐哈尔龙江阜丰生物科技有限公司
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