Method for extracting l-4-hydroxyisoleucine from fermentation catalytic liquid

Abstract

The invention relates to the technical field of separation and purification, in particular to a method for extracting L-4-hydroxyisoleucine from fermentation catalyst liquid. The steps of micro-filtration membrane filtering, super-filtration membrane filtering, ion exchange and the like are used for purifying and separating the L-4-hydroxyisoleucine in the L-4-hydroxyisoleucine fermentation catalyst liquid; the extraction system is simple and efficient; the flow process is simple; the implementation is easy; 732 cation resin adsorption is performed on filtration membrane clear liquid; ammoniawater with the ammonia content being 1.2 to 1.5 weight percent is used for elution; the ammonia salt content in the finished product can be reduced; the purity is improved; the alcohol sedimentation is used in primary crystallization; the yield of the primary coarse product can be improved by 50 percent or higher; in secondary crystallization, the alcohol sedimentation is used, the yield of a finished product can be improved by 50 percent or higher; in addition, the product liquid phase purity can be obviously improved; the final product total yield can reach 50 percent; the product purity isnot less than 95 percent. The method has the advantages that the efficiency is high; the cost is low; the large-scale industrial production is easy.

Description

Technical field [0001] The present invention relates to the field of separation and purification, and more particularly to a method of extracting L-4-hydroxyobine extracting a fermentation catalyst. Background technique [0002] L-4-hydroxyisoleucine (4-hydroxyisyisoleucine) is a non-protein amino acid present in nature. The study found that L-4-hydroxybiobiline has many aspects of promoting insulin secretion, improving insulin tolerance and regulation of blood lipids. The L-4-hydroxyisineluminine can not only promote the intake of glucose, but also reduce liver glucose output to improve insulin tolerance of the outer peripheral tissue. In contrast, insulin synthesis of L-4-hydroxybiobiline regulatory insulin-controlled insulin synthesis is strictly dependent on glucose concentration compared to current drugs that have been used to treat T2DM. Therefore, L-4-hydroxybiobine does not cause side effects such as hypoglycemia. At the same time, the L-4-hydroxybiobine can also effectiv...

AI Technical Summary

Problems solved by technology

[0003] Limited by technology and cost, the output of L-4-hydroxyisoleucine in my country cannot meet the needs of domestic and foreign trade exports

Currently reported L-4-hydroxyisoleucine production methods include extraction, chemical synthesis, and enzymatic methods, but only the extraction method is used for industrial production and has disadvantages such as low extraction rate, difficult separation and purification, large demand for raw materials, and high cost. , the traditional chemical method, whether it is organic synthesis or chemical resolution, loses its competitiveness due to high production costs

In the biological method, the efficiency of the enzyme-catalyzed method is relatively high, but there are still problems such as cumbersome operation, expensive raw materials, and high production cost. At present, there is a lack of relevant technology that can be used in industrialized large-scale production. This method has now been able to formally Carry out large-scale industrial production, and the cost is greatly reduced, and the product meets the detection standard of pharmaceutical grade raw material L-4-hydroxyisoleucine

Images