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Porous fibroin spinal marrow scaffold with directional releasing function as well as preparation method, preparation mold and application thereof

A technology of porous wire and spinal cord, applied in the field of spinal cord scaffolding, can solve the problems of complicated and time-consuming directional freezing operation, and achieve the effects of moderate water absorption and expansion rate, good pore connectivity, and high porosity

Active Publication Date: 2018-07-24
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, directional freezing has disadvantages such as complicated operation and time-consuming

Method used

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  • Porous fibroin spinal marrow scaffold with directional releasing function as well as preparation method, preparation mold and application thereof
  • Porous fibroin spinal marrow scaffold with directional releasing function as well as preparation method, preparation mold and application thereof
  • Porous fibroin spinal marrow scaffold with directional releasing function as well as preparation method, preparation mold and application thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0039] 1. Preparation of silk fibroin aqueous solution

[0040] Take the pupa cocoon and add 0.02mol / L Na with 100 times its mass 2 CO 3 Boil the aqueous solution for 30 minutes, take it out, wring it out, and rinse with distilled water; repeat the above operation twice, dry in an oven at 60°C for 24 hours, and pull loose in the middle to obtain degummed silk; place the degummed silk in a 9.3mol / L LiBr aqueous solution , The mass ratio of degummed silk to 9.3mol / L LiBr aqueous solution is 1:5, keep at 60℃ until the degummed silk is completely dissolved, put it into a dialysis bag with a cut-off molecular weight of 8000-14000 Dalton, and dialyze with tap water at 4℃ for two days. Dialysis with deionized water at 4°C for one day (change the water every 1h on the first day, and every 2 hours for the next two days, and the time interval for changing water at night should not be too long). After dialysis, the solution is filtered through 8 layers of gauze and centrifuged to obtain the ...

Embodiment 1

[0046] In step 1 of preparing the stent in Example 1, 20ml of a mixed solution with a total concentration of 5% and SF / HA=95:5 was prepared, and then 20% of EDC, 10% NHS, and 10% of the silk fibroin mass were added to the mixed solution. 0.012g MES (the content of EDC, NHS, MES is relative to the content of SF in the SF / HA mixed solution), fully stirred for 1h under ice bath. Use a syringe to inject it into the mold (the number of needles is 10), and freeze for 24 hours in a refrigerator at -80°C. The frozen sample was taken out from the mold and placed in a freeze dryer, and dried under reduced pressure for 48 hours to obtain a multi-channel SF / HA porous scaffold.

Embodiment 2

[0048] In step 1 of preparing the stent in Example 1, 20ml of the mixed solution with a total concentration of 5% and SF / HA=90:10 was prepared, and then EDC, 10% NHS, and 10% NHS, which accounted for 20% of the silk fibroin mass, were added to the mixed solution. 0.012g MES (the content of EDC, NHS, MES is relative to the content of SF in the SF / HA mixed solution), fully stirred for 1h under ice bath. Use a syringe to inject it into the mold (the number of needles is 10), and freeze for 24 hours in a refrigerator at -80°C. The frozen sample was taken out from the mold and placed in a freeze dryer, and dried under reduced pressure for 48 hours to obtain a multi-channel SF / HA porous scaffold.

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Abstract

The invention belongs to the technical field of spinal marrow scaffolds and in particular relates to a porous fibroin spinal marrow scaffold with a directional releasing function as well as a preparation method, a preparation mold and application thereof. The method comprises the following steps: 1) preparing a fibroin water solution; preparing a hyaluronic acid solution; 2) mixing the fibroin water solution and the hyaluronic acid solution and adding EDC (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide), NHS (N-hydroxysuccinimide) and MES (2-morpholinoethanesulfonic acid) in sequence and carrying out crosslinking reaction under an ice bath and sufficiently stirring; vacuumizing and de-foaming; then injecting a reaction solution into the preparation mold; putting the mold in a place at -80DEG C or below and freezing and molding; after molding, removing a silicone hose of the mold to obtain a sample; then freezing and drying the sample; after freezing and drying, obtaining the porous fibroin spinal marrow scaffold with the directional releasing function. According to the porous fibroin spinal marrow scaffold with the directional releasing function, a channel is prepared by utilizingan acupuncture needle and the channel can be uniformly and directionally distributed to obtain a fibroin / hyaluronic acid composite porous scaffold with a uniform material pore diameter, adjustable size, high porosity and good pore connectivity.

Description

Technical field [0001] The invention belongs to the technical field of spinal cord stents, and specifically relates to a porous silk fibroin spinal cord stent with directional release function, a preparation method, a preparation mold and an application thereof. Background technique [0002] An ideal scaffold should have the characteristics of suitable pore size, high porosity, good pore connectivity, high mechanical strength, and good biocompatibility. Studies at home and abroad have preliminarily proved that silk fibroin (SF) has certain advantages in repairing nerve tissue defects. Hyaluronic acid (HA) shows a variety of important physiological functions in the body with its unique molecular structure and physical and chemical properties. . Silk fibroin is a protein extracted from silkworm cocoons with the advantages of high mechanical strength, good toughness, and high elasticity. Therefore, combining silk fibroin and sodium hyaluronic acid can overcome the shortcomings of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/50A61L27/54A61L27/56
CPCA61L27/26A61L27/50A61L27/54A61L27/56A61L2430/32C08L89/00C08L5/08
Inventor 张强陈纾闫书芹王秋胜韩国聪杨青青尤仁传徐安长李秀芳
Owner WUHAN TEXTILE UNIV
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