Cysteamine-modified copper nanocluster solution fluorescent probe as well as preparation and application

A fluorescent probe and copper nanotechnology, applied in the field of analysis and detection, can solve problems such as high cost, complex material synthesis, and ecological environmental hazards, and achieve the effects of fast detection, simple operation, and avoidance of interference

Active Publication Date: 2018-07-27
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These materials have good sensitivity and selectivity for the detection of nitroaromatic compounds, but there are some shortcomings, such as complex synthesis of materials and poor reproducibility; materials are not friendly to the environment and cause harm to the ecological environment; synthesis Raw materials are expensive and costly, etc.

Method used

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  • Cysteamine-modified copper nanocluster solution fluorescent probe as well as preparation and application
  • Cysteamine-modified copper nanocluster solution fluorescent probe as well as preparation and application
  • Cysteamine-modified copper nanocluster solution fluorescent probe as well as preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Dissolve 56 mg of ascorbic acid in 20 mL of ultrapure water, add 800 μL of 100 mM copper nitrate solution under stirring, and stir vigorously for 2.5 hours. Then, 18 mg of cysteamine hydrochloride was dissolved in 10 mL of ultrapure water, and the above solution was added under stirring, and after vigorous stirring for 1 hour, the fluorescent probe of cysteamine-modified copper nanocluster solution was prepared for use.

[0026] The cysteamine-modified copper nanocluster solution fluorescent probe synthesized in this example can be viewed under a 365 nm ultraviolet light to show green-blue fluorescence. The emission spectra recorded under the fluorescence spectrometer see figure 1 .

[0027] The application effect test of the cysteamine-modified copper nanocluster solution fluorescent probe in the detection of picric acid obtained in this example:

[0028] Take 100 μL of the probe solution and add it into 1900 μL of ultrapure water, and perform fluorescence detection ...

Embodiment 2

[0030] In this example, the cysteamine-modified copper nanocluster solution fluorescent probe synthesized in Example 1 was applied to the detection of picric acid in real water samples.

[0031] Filter the collected river water, lake water, and tap water twice with qualitative filter paper, and prepare 1mM picric acid solution with the filtered river water, lake water, and tap water respectively. Take 100 μL of the probe solution synthesized in Example 1 and add it into 1900 μL of ultrapure water, and perform fluorescence detection in a quartz cuvette using a Perkin-Elmer LS-55 fluorescence spectrometer. Select the excitation wavelength as 365nm and cut light at 430nm. Add picric acid solution successively so that the picric acid concentration in the solution is 8 μM, 16 μM, 20 μM, 28 μM respectively, record the emission spectrum after each addition of picric acid, and record the fluorescence intensity at 480 nm. Substitute the fluorescence intensity at each concentration int...

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Abstract

The invention belongs to the technical field of analysis and detection and discloses a cysteamine-modified copper nanocluster solution fluorescent probe as well as preparation and an application. Ascorbic acid is dissolved in ultrapure water, a copper ion solution and a cysteamine hydrochloride solution are sequentially added, the mixture is uniformly stirred and mixed, and the cysteamine-modifiedcopper nanocluster solution fluorescent probe is obtained. The cysteamine-modified copper nanocluster solution fluorescent probe is added to ultrapure water to be diluted, a to-be-detected picric acid solution is added, a fluorescence spectrometer is used for detection, and the concentration of picric acid in the to-be-detected solution is analyzed according to the relation between the fluorescence intensity and the concentration of picric acid. The cysteamine-modified copper nanocluster solution is used for detecting picric acid for the first time, and the fluorescence quenching probe is designed to perform fluorescence detection on picric acid, has the advantages of being simple to operate, high in detection speed, good in selectivity and high in sensitivity and has the linear range of0-80 mu mol/L and the detection limit of 0.139 mu mol/L.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a cysteamine-modified copper nano-cluster solution fluorescence probe and its preparation and application. Background technique [0002] Metal nanoclusters refer to relatively stable aggregates composed of several to hundreds of atoms under the protection of a certain molecular layer. Due to its unique quantum size effect, the optical properties of metal nanoclusters vary with particle size, which makes the fluorescence emission spectrum tunable from the near-infrared region to the visible region. Metal nanoclusters also have the advantages of strong stability, good biocompatibility, and large Stokes shift. Copper is widely used due to its high conductivity, similar properties to gold and silver, and lower cost. In recent years, copper clusters have been widely used in biological detection, biomarkers, environmental detection and other fields. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02G01N21/64
CPCC09K11/025G01N21/643G01N2021/6432
Inventor 周海波江正瑾包芷君胡紫微袁凯松简敬一王启钦
Owner JINAN UNIVERSITY
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