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Preparation method and application of self-assembled thrombin esterified microporous hemostatic starch

A technology of microporous starch and esterified microporous, which is applied in the fields of application, pharmaceutical formula, medical science, etc., can solve the problem of no effect for a large amount of bleeding, achieve good biocompatibility, improve hydrophilicity, and improve surface load. The effect of potential

Pending Publication Date: 2018-08-03
苏州和其美生物材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, microporous starch is also used in the field of hemostasis, such as A widely used hemostatic starch that is safe and effective for hemostasis, but ineffective for massive bleeding

Method used

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  • Preparation method and application of self-assembled thrombin esterified microporous hemostatic starch
  • Preparation method and application of self-assembled thrombin esterified microporous hemostatic starch
  • Preparation method and application of self-assembled thrombin esterified microporous hemostatic starch

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A method for preparing self-assembled thrombin esterified microporous hemostatic starch, prepared by the following steps:

[0030] In the first step, 40g of cornstarch is added to 200ml of sodium acetate buffer solution with pH=4, the mixture ratio of α-amylase and glucoamylase contained therein is 1:4, and the ratio of enzyme and starch is 1:20 (w / w), stirred at 25°C at a speed of 150rpm / min for 24h, suction filtered to obtain starch filter residue, dried at 50°C, and ground into powder.

[0031] In the second step, add 10 grams of microporous starch to 100 ml of distilled water, then add 1 g of anhydrous sodium carbonate and 2 g of sodium trimetaphosphate, bathe in water for 48 hours at 25 ° C, filter the sample solution obtained by suction, and put it under 50 ° C Dried and ground into powder.

[0032] The third step is to add 0.2g of the esterified microporous starch obtained by the reaction to 4ml of thrombin aqueous solution, place it at 4°C and continue to vibra...

Embodiment 2

[0034] A method for preparing self-assembled thrombin esterified microporous hemostatic starch, prepared by the following steps:

[0035] In the first step, 40g of cornstarch is added to 200ml of sodium acetate buffer solution with pH=6, the mixture ratio of α-amylase and glucoamylase contained therein is 1:10, and the ratio of enzyme and starch is 1:100 (w / w), stirred at 55°C for 4h at a speed of 550rpm / min, suction filtered to obtain starch filter residue, dried at 85°C, and ground into powder.

[0036]In the second step, add 40 grams of microporous starch to 50 ml of distilled water, then add 0.4 g of anhydrous sodium carbonate and 2 g of sodium trimetaphosphate, bathe in water at 55 ° C for 10 h, filter the sample solution obtained by the reaction, and filter it at 85 °C, and ground into powder.

[0037] In the third step, add 2 g of the esterified microporous starch obtained by the reaction to 4 ml of an aqueous solution of thrombin with an activity of 20 U, place it at ...

Embodiment 3

[0039] A method for preparing self-assembled thrombin esterified microporous hemostatic starch, prepared by the following steps:

[0040] In the first step, 40g of cornstarch is added to 200ml of sodium acetate buffer solution with pH=4.6, the mixture ratio of α-amylase and glucoamylase contained therein is 1:4, and the ratio of enzyme and starch is 1:50 (w / w), stirred at 40°C at a speed of 250rpm / min for 10h, suction filtered to obtain starch filter residue, dried at 60°C, and ground into powder.

[0041] In the second step, add 20 grams of microporous starch to 50 ml of distilled water, then add 0.2 g of anhydrous sodium carbonate and 0.8 g of sodium trimetaphosphate, and put in a water bath at 50 ° C for 24 hours. Dry at 60°C and grind into powder.

[0042] In the third step, add 2 g of the esterified microporous starch obtained by the reaction to 4 ml of an aqueous solution of thrombin with an activity of 20 U, place it at 4°C for 1 hour, and filter it with suction to obt...

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Abstract

The invention relates to a preparation method and application of self-assembled thrombin esterified microporous hemostatic starch. The preparation method comprises the following steps: carrying out heating reaction on microporous starch and sodium trimetaphosphate under the alkaline condition to obtain esterified microporous starch with starch phospholipid on the surface; mixing the esterified microporous hemostatic starch with a thrombin aqueous solution and then carrying out low-temperature drying to obtain the self-assembled thrombin esterified microporous hemostatic starch. Firstly, the hemostatic starch in the invention has a larger surface area and a higher surface negative charge performance, and can be stably combined with a large amount of thrombin through hydrogen bonds. Secondly, the hemostatic starch in the invention can quickly and extensively suck blood, as well as aggregate and activate platelets so as to achieve a rapid hemostatic effect. Thirdly, the hemostatic starchin the invention has no biotoxicity and can be rapidly degraded and absorbed by tissue cells.

Description

technical field [0001] The invention relates to a preparation method for enzymolyzing cornstarch with α-amylase and glucoamylase to obtain microporous starch, esterifying the microporous starch with sodium trimetaphosphate and self-assembling thrombin. And its application in wound hemostasis. It specifically relates to the preparation of hemostatic starch that esterifies microporous starch with sodium trimetaphosphate and self-assembles thrombin, and the application of the method in hemostasis of wound bleeding on body surface and in vivo. . Background technique [0002] It is well known that reducing blood loss after trauma is vital for life and reduces the risk of death from coagulopathy, infection, and multisystem organ failure. Hemostasis has traditionally been done with gauze and suture materials in combination with hemostats; however, while these methods can be used for minor bleeding, they are critical for controlling massive bleeding, especially in organs such as t...

Claims

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Application Information

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IPC IPC(8): A61L24/08A61L24/00
CPCA61L24/001A61L24/0015A61L24/0036A61L24/0042A61L24/08A61L2300/254A61L2300/418A61L2400/04C08L3/16
Inventor 蓝广芊李庆
Owner 苏州和其美生物材料有限公司
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