CARS microscopic imaging apparatus and CARS microscopic imaging method based on photonic crystal optical fiber probe excitation

Abstract

The invention discloses a CARS microscopic imaging apparatus and a CARS microscopic imaging method based on photonic crystal optical fiber probe excitation. The CARS microscopic imaging apparatus comprises a femtosecond laser device input light source, a pump light pulse optical path, a Stokes optical pulse optical path, high nonlinear polarization-maintaining photonic crystal optical fiber, a to-be-detected sample, a light collection objective lens and a signal detection and data processing system. According to the present invention, the high nonlinear polarization-maintaining photonic crystal optical fiber is used as the Stokes optical pulse generation device in the CARS microscopic imaging system, and is further used as the light guiding device of the pump light pulse in the system so as to make the pump light and the Stokes light output by the high nonlinear polarization-maintaining photonic crystal optical fiber be directly incident into the surface of the to-be-detected sample, the intensity and the spatial distribution of the anti-Stokes Raman scattering signal are analyzed, and the spatial distribution of the specific chemical bond in the to-be-detected sample is reconstructed; and compared to the apparatus in the prior art, the apparatus of the present invention has advantages of system structure simplifying, convenient system operation and improved system integration,and can help to push the CARS microscopic imaging system from scientific research to practical application.

Description

technical field [0001] The invention belongs to the field of nonlinear optics and microscopic imaging, and relates to a CARS microscopic imaging method and device based on photonic crystal fiber light guiding and probe excitation. Background technique [0002] Due to the effect of light wave diffraction, ordinary optical microscopes often have a theoretical limit in spatial resolution and cannot perform chemical selective imaging. Therefore, it is proposed to improve the imaging quality, improve the image clarity, and increase the chemical selectivity by increasing the contrast between the object image and the background. [0003] Coherent Anti-Stokes Raman Scattering (CARS) imaging technology is a non-contact microscopic imaging technology that uses the resonance energy level of molecules in substances to detect, and its essence is four-wave mixing third-order nonlinear process. CARS microscopic imaging technology has the advantages of high spatial resolution, high detect...

AI Technical Summary

Problems solved by technology

The traditional CARS microscopic imaging system has shortcomings such as bulky, cumbersome adjus

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