Steamed polygonatum rhizome quality detection and analysis method

A technology of quality inspection and analysis method, which is applied in the field of quality inspection of steamed sealwort, can solve the problems of lack of unified and reliable detection and analysis methods, difficulty in distinguishing the quality of different sealworts, and difficulty in judging the quality grade of sealwort, so as to promote technological innovation and improve inspection Analysis on the quality of sealwort and the effect of guaranteeing benefits

Pending Publication Date: 2018-08-03
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although Chinese medicine researchers are aware of the above two processing techniques, there is a lack of unified and reliable detection and analysis methods for the specific quality and attributes of Polygonatum products obtained by different processing techniques
Therefore, it is difficult for Chinese medicinal raw material processing enterprises and researchers to judge the quality grade of the processed Polygonatum; at the same ti

Method used

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  • Steamed polygonatum rhizome quality detection and analysis method
  • Steamed polygonatum rhizome quality detection and analysis method
  • Steamed polygonatum rhizome quality detection and analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Quality detection and analysis of steamed Polygonatum

[0079] Get fresh steamed sealwort, observe the color and luster of the appearance with the naked eye, and it is unqualified if there is blackening, deterioration, and corruption.

[0080] For qualified steamed Polygonatum samples, the 5-HMF content, diosgenin content, polysaccharide content, water-soluble extract content, ash content, and moisture content in steamed Polygonatum were further detected.

[0081] (1) Determination of 5-HMF content in Polygonatum:

[0082] 101: Preparation of reference substance solution, accurately weigh 5-HMF reference substance, dissolve in methanol to make reference substance solution, and store in low temperature in the dark.

[0083] 102: Prepare the test solution, accurately weigh Polygonatum, add 10-20 times the weight of methanol for ultrasonic extraction for 10-30 minutes, filter, add 10-20 times the weight of methanol for ultrasonic extraction for 10-30 minutes, and filter...

Embodiment 2

[0094] Determination of 5-HMF content in Polygonatum:

[0095] 1 Prepare the reference substance solution, accurately weigh 5-HMF reference substance, dissolve it in methanol to make the reference substance solution, and store it in the dark and at low temperature.

[0096] 2 Preparation of the test solution, accurately weigh Polygonatum, add 10mL methanol for ultrasonic extraction for 10-30min, filter, add 10mL methanol for ultrasonic extraction for 10-30min to filter residue, and filter. Combine the filtrates and dilute to a 25mL volumetric flask.

[0097] 3 Use HPLC to test the reference substance solution and the test solution, and detect under the following chromatographic conditions: Elite ODS-B chromatographic column (250mm×4.6mm, 5μm); mobile phase: acetonitrile: 1% phosphoric acid water (5:95) ; Flow rate: 1.0ml / min; Detection wavelength: 284nm; Column temperature: 30°C; Injection volume: 10 μL. Calculate the 5-HMPF content in Polygonatum.

Embodiment 3

[0099] Determination of Diosgenin in Polygonatum:

[0100] 1: Prepare the reference substance solution, accurately weigh the diosgenin reference substance, dissolve the prepared reference substance in methanol, and store it in the dark and at low temperature. Preferably, keep away from light and store at a low temperature of 1-6°C.

[0101] 2: For the preparation of the test solution, weigh Polygonatum powder, add ethanol, shake well, weigh, heat and reflux for 2-5h, let cool, weigh, use ethanol to fill the weight, shake well, and filter. Concentrate the filtrate to dryness, add 1-4mol / L hydrochloric acid to the residue to dissolve, reflux with boiling water for 2-5h, cool, move to a separatory funnel, add chloroform, extract 2-5 times, combine the chloroform solution, concentrate to dryness under reduced pressure, Add the residue to dissolve in methanol, dilute to 25mL volumetric flask, and shake well to obtain the test solution.

[0102] 3: Using HPLC to test the reference...

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Abstract

The invention discloses a steamed polygonatum rhizome quality detection and analysis method. The method comprises the following steps: taking fresh steamed polygonatum rhizome, and investigating appearance color, blackening deterioration and rot of materials; and performing further detection on 5-HMF content, diosgenin content, polysaccharide content, water-soluble leaching object content, ash content and moisture content in a qualified steamed polygonatum rhizome sample. The steamed polygonatum rhizome quality detection and analysis method aims at shortage of an uniform standard for evaluating quality of the steamed polygonatum rhizome, by combining with morphology and the quality characteristic of the steamed polygonatum rhizome, a proper detection analysis mode and parameter condition can be screened, the detection analysis result can accurately reflect the quality of the steamed polygonatum rhizome, provides the guidance for traditional Chinese medicinal material processing enterprises, and provides reference for choosing the high-quality steamed polygonatum rhizome for consumers.

Description

technical field [0001] The invention relates to a method for detecting the quality of steamed Polygonatum, which belongs to the technical field of processing traditional Chinese medicinal materials. Background technique [0002] Polygonati rhizoma, Polygonati rhizoma, is a plant of Liliaceae. This product is the dried rhizome of Polygonati rhizoma, Polygonati rhizoma or Polygonatum multiflora. According to different shapes, it is commonly called "rhubarb essence", "chicken head polygonatum essence" and "ginger-shaped polygonatum essence". Polygonatum, sweet in taste and flat in nature, returns to the spleen, lung and kidney channels. Tonify qi and nourish yin, invigorate the spleen, moisten the lungs, and benefit the kidneys. For spleen and stomach qi deficiency, body fatigue and fatigue, insufficient stomach yin, dry mouth and lack of food, dry cough due to lung deficiency, labor cough and hemoptysis, insufficient essence and blood, sore waist and knees, premature graying...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 彭腾韩笑白玲张瑶匡于党凯茹赵永艳向本超冯雨琴刘继红
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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