Adsorbent used for clearing middle and macro molecule toxin in body of uremia patients and preparation method thereof

Abstract

The invention relates to an adsorbent used for clearing middle and macro molecule toxin in body of uremia patients and a preparation method thereof. According to the molecule size of middle and macromolecules such as beta2-MG, PTH, AGEs, Hcy and IL-6 and performance characteristics thereof, a functional adsorbent which can efficiently clear main middle and macro molecule toxins such as beta2-MG,PTH, AGEs, Hcy and IL-6 in the blood of the uremia patients is prepared by taking a mesoporous polystyrene hydrophobic resin as a carrier, which undergoes chloromethylation activation, and then performing chemical crosslinking grafting of vinylamine; and the adsorbent has large adsorption capacity, good mechanical strength, good biocompatibility and high adsorption selectivity. The adsorbent is used for treating uremia by blood perfusion, can take the advantages of blood filtering mainly for clearing micromolecular toxin, and provides a new method for treating uremia by combination of hematodialysis and blood perfusion.

Description

Technical field:

[0001] The invention belongs to the technical field of biomedicine. It specifically relates to a novel medical adsorbent, especially an adsorbent suitable for hemoperfusion to remove medium and macromolecular toxins from the body of uremia patients and a preparation method thereof. technical background:

[0002] Uremia is a series of complex clinical syndromes caused by progressive and irreversible decline of renal function caused by various kidney diseases in the body, which eventually leads to loss of renal function, which prevents timely discharge of metabolic waste in the body and accumulates in large quantities. Uremia may lead to complications such as water-electrolyte and acid-base metabolism disorders, cardiovascular diseases, nervous system diseases, respiratory diseases, gastrointestinal diseases, skin itching, etc., seriously affecting the physical and mental health and life safety of patients. At present, it is known that there are more than 200...

Examples

Embodiment 1

[0026] (1) Preparation of carrier microspheres

[0027]15.4g styrene and 84.6g divinylbenzene were added to a 1000mL beaker, then 327.3g toluene, 109.1g ethyl acetate, 43.6g gasoline and 6.96g benzoyl peroxide were added, stirred to dissolve, and After the dissolution is complete, mix thoroughly to obtain an organic phase. At normal temperature, 11.02g of polyvinyl alcohol and 580mL of deionized water were added to a 2000mL three-neck flask to form an aqueous solution of 1.9% (mass fraction) of polyvinyl alcohol, and heated and stirred to 45°C to fully dissolve the polyvinyl alcohol to obtain Water phase, stop stirring, add the obtained uniformly mixed organic phase into the water phase, start the stirrer, adjust the stirring rate, so that the droplets are evenly distributed, after the temperature is raised to 50°C, the temperature is raised to 80°C at 5°C / 10min , to make the liquid titrate, keep it at this temperature for 3 hours after being finalized, then slowly raise the ...

Embodiment 2

[0033] (1) Preparation of carrier microspheres

[0034] Add 30g of styrene and 120g of divinylbenzene into a 1000mL beaker, then add 189.5g of toluene, 63.2g of ethyl acetate, 47.3g of gasoline and 4.5g of benzoyl peroxide, stir to dissolve, and wait until it is completely dissolved Afterwards, fully mix to obtain the organic phase. At room temperature, add 9g of polyvinyl alcohol and 900mL of deionized water into a 2000mL three-necked flask to form a 1% (mass fraction) aqueous solution of polyvinyl alcohol, and heat and stir to 45°C to fully dissolve the polyvinyl alcohol to obtain water Phase, stop stirring, add the obtained uniformly mixed organic phase into the water phase, start the stirrer, adjust the stirring rate, so that the droplets are evenly distributed, after the temperature is raised to 46°C, the temperature is raised to 75°C at 4°C / 10min, Make the solution titrate, keep it at this temperature for 4 hours after being finalized, then slowly raise the temperature ...

Embodiment 3

[0040] (1) Preparation of carrier microspheres

[0041] Add 28.6g of styrene and 71.4g of divinylbenzene into a 1000mL beaker, then add 120g of toluene, 30g of ethyl acetate, 30g of gasoline and 2g of benzoyl peroxide, and stir to dissolve it. After the dissolution is complete, Mix well to obtain an organic phase. At normal temperature, 7.5g of polyvinyl alcohol and 450mL of deionized water were added to a 2000mL three-necked flask to form an aqueous solution of 1.5% (mass fraction) of polyvinyl alcohol, and heated and stirred to 45°C to fully dissolve the polyvinyl alcohol to obtain Water phase, stop stirring, add the obtained uniformly mixed organic phase into the water phase, start the stirrer, adjust the stirring rate, so that the droplets are evenly distributed, after the temperature is raised to 42 ° C, the temperature is raised to 70 ° C at 3 ° C / 10 min , to make the liquid titrate, keep it at this temperature for 3.5 hours after being finalized, then slowly raise th...

Technical field:

[0001] The invention belongs to the technical field of biomedicine. It specifically relates to a novel medical adsorbent, especially an adsorbent suitable for hemoperfusion to remove medium and macromolecular toxins from the body of uremia patients and a preparation method thereof. technical background:

[0002] Uremia is a series of complex clinical syndromes caused by progressive and irreversible decline of renal function caused by various kidney diseases in the body, which eventually leads to loss of renal function, which prevents timely discharge of metabolic waste in the body and accumulates in large quantities. Uremia may lead to complications such as water-electrolyte and acid-base metabolism disorders, cardiovascular diseases, nervous system diseases, respiratory diseases, gastrointestinal diseases, skin itching, etc., seriously affecting the physical and mental health and life safety of patients. At present, it is known that there are more than 200...

Examples

Embodiment 1

[0026] (1) Preparation of carrier microspheres

[0027]15.4g styrene and 84.6g divinylbenzene were added to a 1000mL beaker, then 327.3g toluene, 109.1g ethyl acetate, 43.6g gasoline and 6.96g benzoyl peroxide were added, stirred to dissolve, and After the dissolution is complete, mix thoroughly to obtain an organic phase. At normal temperature, 11.02g of polyvinyl alcohol and 580mL of deionized water were added to a 2000mL three-neck flask to form an aqueous solution of 1.9% (mass fraction) of polyvinyl alcohol, and heated and stirred to 45°C to fully dissolve the polyvinyl alcohol to obtain Water phase, stop stirring, add the obtained uniformly mixed organic phase into the water phase, start the stirrer, adjust the stirring rate, so that the droplets are evenly distributed, after the temperature is raised to 50°C, the temperature is raised to 80°C at 5°C / 10min , to make the liquid titrate, keep it at this temperature for 3 hours after being finalized, then slowly raise the ...

Embodiment 2

[0033] (1) Preparation of carrier microspheres

[0034] Add 30g of styrene and 120g of divinylbenzene into a 1000mL beaker, then add 189.5g of toluene, 63.2g of ethyl acetate, 47.3g of gasoline and 4.5g of benzoyl peroxide, stir to dissolve, and wait until it is completely dissolved Afterwards, fully mix to obtain the organic phase. At room temperature, add 9g of polyvinyl alcohol and 900mL of deionized water into a 2000mL three-necked flask to form a 1% (mass fraction) aqueous solution of polyvinyl alcohol, and heat and stir to 45°C to fully dissolve the polyvinyl alcohol to obtain water Phase, stop stirring, add the obtained uniformly mixed organic phase into the water phase, start the stirrer, adjust the stirring rate, so that the droplets are evenly distributed, after the temperature is raised to 46°C, the temperature is raised to 75°C at 4°C / 10min, Make the solution titrate, keep it at this temperature for 4 hours after being finalized, then slowly raise the temperature ...

Embodiment 3

[0040] (1) Preparation of carrier microspheres

[0041] Add 28.6g of styrene and 71.4g of divinylbenzene into a 1000mL beaker, then add 120g of toluene, 30g of ethyl acetate, 30g of gasoline and 2g of benzoyl peroxide, and stir to dissolve it. After the dissolution is complete, Mix well to obtain an organic phase. At normal temperature, 7.5g of polyvinyl alcohol and 450mL of deionized water were added to a 2000mL three-necked flask to form an aqueous solution of 1.5% (mass fraction) of polyvinyl alcohol, and heated and stirred to 45°C to fully dissolve the polyvinyl alcohol to obtain Water phase, stop stirring, add the obtained uniformly mixed organic phase into the water phase, start the stirrer, adjust the stirring rate, so that the droplets are evenly distributed, after the temperature is raised to 42 ° C, the temperature is raised to 70 ° C at 3 ° C / 10 min , to make the liquid titrate, keep it at this temperature for 3.5 hours after being finalized, then slowly raise th...

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