Method for preparing ganoderma triterpenes
A technology of Ganoderma lucidum triterpenes and Ganoderma lucidum alcohol, which is applied in the field of deep processing of edible and medicinal fungi, can solve the problems of many organic solvents, low extraction efficiency, and high cost, and achieve the effects of improved extraction efficiency, high biological activity, and enhanced activity
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Embodiment 1
[0023] The fruit body of Hunong Ganoderma lucidum (G.lucidum) No. 1 (Shanghai Academy of Agricultural Sciences), whose origin is Longquan, Zhejiang, is crushed and passed through a 20-mesh sieve, weighed 1000g and placed in an extraction tank, and added 80% ethanol, the liquid-material mass ratio 40:1, extraction temperature 85°C, after 3 hours of extraction, the extract was filtered and concentrated to obtain 88.83 g of ethanol extract of Ganoderma lucidum, marked as I, which contained 10% ethanol.
[0024] Process the fruiting body of Ganoderma lucidum into a particle size of 60 mesh, add the prepared extract-like ethanol extract I of Ganoderma lucidum and the fruiting body of Ganoderma lucidum at a mass ratio of 1:1 and mix, then weigh 40g of the mixture and put it into a supercritical extraction tank Inside, supercritical CO 2 Extraction, the extraction process conditions are: temperature 40°C, pressure 30Mpa, 90% ethanol entrainment, entrainer dosage 6mL / g, time 1h.
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Embodiment 2
[0033] The fruiting body of Hunong Ganoderma lucidum (G.lucidum) No. 1 (Shanghai Academy of Agricultural Sciences), whose origin is Longquan, Zhejiang, is crushed and passed through a 10-mesh sieve, weighed 1000g and placed in an extraction tank, and added 90% ethanol, the liquid-material mass ratio The extraction temperature was 40:1, the extraction temperature was 90°C, and after extraction for 2 hours, it was filtered and concentrated to obtain 81.49 g of ethanol extract of Ganoderma lucidum, marked as II, which contained 5% ethanol.
[0034] Process the fruiting body of Ganoderma lucidum into a particle size of 80 meshes, add the prepared extract-like ethanol extract II of Ganoderma lucidum and the fruiting body of Ganoderma lucidum according to the mass ratio of 1:1 and mix, then weigh 40g of the mixture and put it into a supercritical extraction tank Inside, supercritical CO 2 Extraction, the extraction process conditions are: temperature 50°C, pressure 35Mpa, 90% ethano...
Embodiment 3
[0037] Inhibition rate detection of LNCaP cells
[0038] Cell concentration was 5 4 Each / mL of tumor cell LNCaP (purchased from the Cell Resource Center, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences) suspension 180 μL was added to a 96-well plate, and after 48 hours, 20 μL of the sample to be tested (Ganoderma lucidum extract in Example 1, heat reflux extraction, indexing Ganoderma lucidum extract prepared by the four methods of extraction, ultrasonic extraction and cold soaking extraction), DMSO was used as negative control, finasteride was used as positive control, each sample was set in 3 replicates, at 37 ° C, 5% concentration CO 2 After culturing for 48 hours under the same conditions, suck off the old medium and add 180 μL of colorless 1640 medium (Thermo Fisher Scientific, USA), 20 μL of 0.075 mg / mL Alamar Blue reagent (Thermo Fisher Scientific, USA), and use ELISA after the color changes. The detector measures the absorbance at 570 and 600nm...
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